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Useful publications

A selection of peer-review scientific papers which review methodologies, the uses and statistical considerations for the comet assay. We hope this will be a useful starting point for researchers who are new to the comet assay.

A Standardized Protocol for the In Vitro Comet-Based DNA Repair Assay
(2014) J Slyskova et al. Methods in Pharmacology and Toxicology 2014, (II) 377-395

Comet assay to measure DNA repair: approach and applications
(2014) A. Azqueta et al,Front. Genet.,5:288.

On the search for an intelligible comet assay descriptor
(2014) P Moller et al. Front. Genet., 5:217.

Towards a more reliable comet assay: Optimising agarose concentration, unwinding time and electrophoresis conditions
(2011) A. Azqueta et al, Mut Res. 724 (1-2):41-45

Use of Comet-FISH in the study of DNA damage and repair: Review
(2009) M. Glei et al, Mut. Res. 681(1):33-43

Introducing a true internal standard for the Comet assay to minimize intra- and inter-experiment variability in measures of DNA damage and repair.
(2009) M. Zainol et al, Nucleic Acids Res. 2009 Dec;37(22):e150.

Statistical issues in the use of the comet assay
(2008) D.P. Lovell et al, Mutagenesis 23(3):171-182

The comet assay: topical issues
(2008) A.R. Collins et al, Mutagenesis 23(3):143-151

The use of the comet assay in the study of human nutrition and cancer
(2008) G.R. Wasson et al, Mutagenesis 23(3):153-162

Use of single cell gel electrophoresis assays for the detection of DNA-protective effects of dietary factors in humans: Recent results and trends
(2008) C. Hoetzl et al, Mut. Res. 681(1):68-79

Ecotoxicological applications and significance of the comet assay
(2008) A.N. Jha, Mutagenesis 23(3):207-21

Environmental and occupational biomonitoring using the Comet assay
(2008) M. Valverde and E. Rojas, Mut. Res. 681(1):93-109

The Comet assay for the evaluation of genotoxic impact in aquatic environments
(2008) G. Frenzilli et al, Mut. Res. 681(1):80-92

New applications of the Comet assay: Comet–FISH and transcription-coupled DNA repair
(2008) G. Spivak et al, Mut. Res. 681(1):44-50

Investigating oxidative DNA damage and its repair using the comet assay
(2008) A.R. Collins, Mut. Res. 681(1):24-32

Impact of the comet assay in radiobiology
(2008) P.L. Olive, Mut. Res. 681(1):13-23

Potential use of the comet assay in the clinical management of cancer
(2008) D.J. McKenna et al, Mutagenesis 23(3):183-90

Fourth International Workgroup on Genotoxicity testing: results of the in vivo Comet assay workgroup
(2007) B. Burlinson et al, Mutat. Res. 627(1):31-35

The in vivo comet assay: use and status in genotoxicity testing
(2005) S. Brendler-Schwaab et al, Mutagenesis 20(4):245-254

The comet assay: A biomonitoring tool for nutraceutical research
(2005) V.W.C. Wong, C. T. Nut. Res. 3(1):1-14

The comet assay for DNA damage and repair: principles, applications, and limitations
(2004) A. Collins, Mol. Bio. 26(3):249-261

Use of the alkaline in vivo Comet assay for mechanistic genotoxicity investigations
(2004) A. Hartmann et al, Mutagenesis 19(1):51-59

Aspects of design and statistical analysis in the Comet assay
(2003) S.J. Wiklund and E. Agurell, Mutagenesis 18(2):167-175

Recommendations for conducting the in vivo alkaline Comet assay
(2003) A. Hartmann et al, Mutagenesis 18(1):45-51

Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the "comet" assay
(1990) P.L. Olive et al, Radiat. Res. 122(1):86-94