Papers'18

Papers from 2018

Chemopreventive potential of in vitro fermented raw and roasted hazelnuts in LT97 colon adenoma cells
M Glei, S Fischer, J Lamberty, D Ludwig… - Anticancer Research, 2018
… After staining DNA with SYBR® Green (Sigma Aldrich, Munich, Germany) DNA damage was detected using a fluorescence microscope (ZEISS Axiostar plus; Carl Zeiss Jena GmbH) and image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK) …

Comprehensive evaluation of the flavonol anti-oxidants, alpha-glycosyl isoquercitrin and isoquercitrin, for genotoxic potential
CA Hobbs, M Koyanagi, C Swartz, J Davis, S Kasamoto… - Food and Chemical Toxicology …, 2018
… slides with SYBR Gold™ (Molecular Probes, Invitrogen, Carlsbad, CA), 150 cells were scored per sample at 200× total magnification without scorers' knowledge of animal identity using Comet Assay IV Imaging Software, Version 4.3.1 (Perceptive Instruments, Ltd., Suffolk, UK) …

Persistent Organochlorine Pollutants in Placentas Sampled from Women in Croatia and an Evaluation of Their DNA Damaging Potential In Vitro
D Želježic, SH Romanic, D Klincic, MM Saric, JG Letinic - Archives of Environmental Contamination and Toxicology, 2018
… Neutralisation of slides was done by redistilled water, and they were stained with ethidium bro- mide (20 µg mL-1). The analysis of primary DNA damage was performed using Comet Assay IV system (Perceptive Instruments Ltd., Suffolk, Halstead, UK) connected to a …

Effect of Slide Positioning in Electrophoresis Chamber over Comet Assay Results,
M Roy, A Hamel, R Cardoso, 2018
… using a fluorescence microscope. The computerized image analysis Comet Assay IV from Perceptive Instruments was used for the analysis. 75 cells (lung) or 150 cells (duodenum) per slide were analyzed. The median % tail …

Investigation of IN vitro genotoxic effects of an anti-diabetic drug sitagliptin
D Yuzbasioglu, C Enguzel-Alperen, F Unal - Food and Chemical Toxicology, 2018
… were examined at 400 × magnification using the fluorescence microscope (Olympus, Center Valley, PA, USA) equipped with an excitation filter of 546 nm and a barrier filter of 590 nm and specialized Image Analysis System (“Comet Assay IV”, Perceptive Instruments Ltd., UK) …

Crucial role of chelatable iron in silver nanoparticles induced DNA damage and cytotoxicity
A Grzelak, M Wojewódzka, S Meczynska-Wielgosz… - Redox Biology, 2018
… above. Image analysis of the data was performed with the Comet Assay IV Image Analysis System (Perceptive Instruments, UK). Fifty randomly selected comets per slide were analysed, two slides per experimental point. The …

The PARP inhibitor AZD2461 is differentiated from olaparib for both PARP3 activity and drug resistance
LO O'Connor, SL Rulten, AN Cranston, R Odedra…, 2018
… assay as described (31). Comet tail moments were scored from 100 cells per experiment using Comet Assay IV (Perceptive Instruments). Two-way analysis of variance was carried out on the mean values for each time point from replicate experiments. Immunofluorescence …

DNA damage in blood cells in relation to chemotherapy and nutritional status in colorectal cancer patients—A pilot study
AS Kværner, J Minaguchi, N El Yamani, C Henriksen… - DNA Repair, 2018
… They were then washed twice with water and left to dry. Comets were scored with Comet Assay IV software (Perceptive Instruments) and results expressed as % tail DNA. Highly damaged cells were not excluded from the analysis. 2.5. Statistical analysis. 2.5.1. Main analyses …

Genotoxicity of fine and coarse fraction ambient particulate matter in immortalised normal (TT1) and cancer-derived (A549) alveolar epithelial cells
IWH Jarvis, Z Enlo-Scott, E Nagy, IS Mudway… - Environmental and Molecular Mutagenesis, 2018
… Nuclei were stained with ethidium bromide (2 µg/ml in water) and washed in deionized water. A total of 50 comets were scored using a Leica DMLB fluorescent microscope and Comet IV capture system (Perceptive Instruments, UK) …

Assessment of the Predictive Capacity of the Optimized in vitro Comet Assay Using HepG2Cells
Y Hong, HL Jeon, KY Ko, J Kim, JS Yi, I Ahn, TS Kim… - Mutation Research/Genetic …, 2018
… The cells were observed at 20 × magnification with a Leica DM 4000 B fluorescence microscope (Leica, Wetzler, Germany) and analyzed using Comet Assay IV digital imaging software (Perceptive Instruments, Suffolk, UK) …

Genotoxicity study of Ethiopian medicinal plant extracts on HepG2 cells
W Kahaliw, B Hellman, E Engidawork - BMC Complementary and Alternative …, 2018
… staining of the slides, detection was performed with an Olympus BX60 fluorescence microscope with an AVT FireWire camera (Stingray; Allied Technologies, Germany), a CoolLED pE-100 excitation light (536 nm) and the software Comet Assay IV (Perspective instruments, UK) …

Asymptomatic HIV-people present different profile of sCD14, sRAGE, DNA damage and vitamins, according to the use of cART and CD4+ T cells restoration.
KI Tasca, CR Correa, JT Caleffi, MB Mendes, M Gatto…, Journal of Immunology Research, 2018
… Using an immunofluorescence microscope connected to an image analysis system (comet assay IV, Perceptive Instruments, Sufolk, Haverhill, UK), a total of 50 randomly selected nucleoids were counted for each slide. Results were expressed as …

Light-based Methods for Whole Blood Bacterial Inactivation Enabled by a Recirculating Flow System
G Kim, M Karbaschi, M Cooke, A Gaitas - Photochemistry and Photobiology, 2018
… damage. All slides were then observed and fifty comets per gel selected and scored using a fluorescence microscope (Axioskop; Carl Zeiss, Jena Germany) and Comet Assay IV analysis software (version 4.2; Perceptive Instruments, Haverhill, Suffolk, UK). Analyses …