Papers'19

Papers from 2019

Spatial and temporal variation of three biomarkers in Mytilus edulis
S Strubbia, BP Lyons, RJ Lee - Marine Pollution Bulletin, 2019
...The tails were evaluated through Comet Assay IV analysis software (Perceptive Instruments Ltd) and Camera AVT smart view for, Fire Package version 1.6.2) to measure the total image fluorescence intensity, head intensity, tail intensity, head length and tail length. The results were recorded as the % tail intensity over 50 cells...

Poly (e-caprolactone) microspheres for prolonged release of selenium nanoparticles
N Filipovic, L Veselinovic, S Razic, S Jeremic, M Filipic… - Materials Science and Engineering, 2019
... Images of 50 randomly selected nuclei per experimental point were analyzed with image analysis software Comet Assay IV (Perceptive Instruments, UK). The percentages of tail DNA were used to measure the levels of DNA damage …

Multivitamin and Mineral Supplementation Containing Phytonutrients Scavenges Reactive Oxygen Species in Healthy Subjects: A Randomized, Double-Blinded, Placebo-Controlled Trial
S Kang, Y Lim, Y Kim, E Jung, D Suh, C Lee, E Park… - Nutrients, 2019
… Comet assay was performed using a single-cell gel electrophoresis assay as previously described [23] … Images from 50 comets on each slide were analyzed with COMET VI image analysis software (Perceptive Instruments, Suffolk, UK) …

Application of Crayfish Astacus Leptodactylus for the Analysis of Water Genotoxicity in the Lake Sevan Basin
AE Simonyan, AA Sargsyan, GG Hovhannisyan… - Journal of Water Chemistry and TEchnology, 2018
… microscope (ZEISS, Germany). The estimation of DNA damages was performed by using commercial software Comet Assay VI (Perceptive Instruments, UK). 150 cells were analyzed for each crayfish. The statistical processing …

Cytotoxic, genotoxic, and oxidative stress-inducing effect of an l-amino acid oxidase isolated from Bothrops jararacussu venom in a co-culture model of HepG2 and HUVEC cells
ART Machado, AF Aissa, DL Ribeiro, TR Costa… - International Journal of Biological Macromolecules, 2019
… One hundred nucleoids per sample were analyzed with the aid of the Comet Assay IV™ software (Perceptive Instruments, Haverhill, UK). Percentage (%) of DNA in the tail (Tail Intensity) was used as a parameter of DNA damage …

Efficient Single-Strand Break Repair Requires Binding to Both Poly (ADP-Ribose) and DNA by the Central BRCT Domain of XRCC1
LM Polo, Y Xu, P Hornyak, F Garces, Z Zeng… - Cell Reports, 2019
Finally, DNA was stained with SybrGreen I (1:10,000 in PBS) for 30 min. Average tail moments from 100 cells/sample were measured using Comet Assay IV software (Perceptive Instruments, UK). Data are the average ± 1?SEM of three independent experiments and were scored blind.

The CHD6 chromatin remodeler is an oxidative DNA damage response factor
S Moore, ND Berger, MS Luijsterburg, CG Piett… - Nature Communications, 2019
Comet scoring was performed on a Zeiss Axiovision microscope with a camera associated with Comet Assay IV software (Perceptive Instruments). A total of 100 comets were scored per condition, per repeat the data expressed as the average comet tail moment on a scatter plot.

TA new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis
SB Colovic, O Jankovic, S Živkovic, Ž Žižak, IB Žižak… - Ceramics International, 2019
For statistical analysis of the results of comet assay, Comet assay IV software (Perceptive Instruments) was used

Oxygen Availability during Growth Modulates the Phytochemical Profile and the Chemo-Protective Properties of Spinach Juice
F Milano, F Mussi, S Fornaciari, M Altunoz, L Forti… - Biomolecules, 2019
Slides were examined at 400× magnification under a Leica DMLS fluorescence microscope (excitation filter BP 515–560 nm, barrier filter LP 580 nm), using an automatic image analysis system (Comet Assay III

The WD40 domain of FBXW7 is a poly (ADP-ribose)-binding domain that mediates the early DNA damage response
Q Zhang, ASA Mady, Y Ma, C Ryan, TS Lawrence… - Nucleic Acids Research, 2019
Then, the slides were subjected to electrophoresis at 20 V (0.6 V/cm) for 30 min and stained in 2.5 µg/ml propidium iodide for 20 min. All images were taken with a fluorescence microscope and analyzed by Comet Assay IV software (Perceptive Instruments).

miR-205 enhances radiation sensitivity of prostate cancer cells by impairing DNA damage repair through PKCe and ZEB1 inhibition
R El Bezawy, S Tinelli, M Tortoreto, V Doldi, V Zuco… - Journal of Experimental & Clinical Cancer Research…, 2019
… Comets were imaged using a fluorescence microscope equipped with a video camera (Jai Pulnix, Sunnyvale, CA), and quantitative assessment of DNA damage was obtained using the Comet Assay IV software (Perceptive Instruments, Suffolk, UK) …

The comet assay for human biomonitoring: Effect of cryopreservation on DNA damage in different blood cell preparations
C Ladeira, G Koppen, F Scavone, L Giovannelli - Mutation Research/Genetic …, 2019
… distribution graphs were performed and generated with Comet Assay IV® software (Perceptive Instruments) … different blood preparation for use in biomonitoring with the comet assay, with particular … The latter have been …

Copper (II) complexes with N'-methylsarcosinamide selective for human bladder cancer cells
N Smrečki, T Rončević, O Jović, BM Kukovec… - Inorganica Chimica …, 2019
analysed at × 250 magnification using an epifluorescence microscope (Zeiss, Germany) connected to an image analysis system (Comet Assay II; Perceptive Instruments Ltd., UK). ….

Moderate temperature elevation increase susceptibility of early-life stage of the Mediterranean mussel, Mytilus galloprovincialis to metal-induced genotoxicity
K Boukadida, J Cachot, B Morin, C Clerandeau… - Science of The Total Environment 2019
Comet Assay IV software (Perceptive instruments, Bury St Edmunds, UK). One hundred nuclei per gel were analyzed, and DNA damage was expressed as a percentage of DNA in the comet's tail...

An Adaption of Human-Induced Hepatocytes to In Vitro Genetic Toxicity Tests
W Liu, J Xi, Y Cao, X You, R Chen, X Zhang… - Mutagenesis, 2019
At least 100 cells per concentration were analysed using Comet Assay IV software (Perceptive Instruments, Haverhill, UK).The extent of DNA damage in individual cells was evaluated as the % Tail DNA …

Cell–cell contacts protect against t-BuOOH-induced cellular damage and ferroptosis in vitro
C Wenz, D Faust, B Linz, C Turmann, T Nikolova… - Archives of Toxicology, 2019
...At least 50 cells/slide were scored using the Comet IV software (Perceptive Instruments Ltd., Bury St Edmunds, UK).

Dark coffee consumption protects human blood cells from spontaneous DNA damage
G Pahlke, E Attakpah, G Aichinger, K Ahlberg… - Journal of Functional Foods, 2019
...Slides were subjected to computer-aided image analysis (Comet Assay IV System, Perceptive Instruments, Suffolk, Great Britain), scoring 200 randomly picked cells per slide…

Anti-inflammatory and antioxidative properties of honey bee venom on Freund's Complete Adjuvant-induced arthritis model in rats
A Kocyigit, EM Guler, S Kaleli - Toxicon, 2019
… A computerized image analysis system (Comet Assay IV; Perceptive Instruments) was used to evaluate the degree of DNA damage. The percentage of DNA in the tail intensity (tail intensity %) was used as the …

Shape-engineered titanium dioxide nanoparticles (TiO2-NPs): cytotoxicity and genotoxicity in bronchial epithelial cells
M Gea, S Bonetta, L Iannarelli, AM Giovannozzi… - Food and Chemical Toxicology, 2019
… The percentage of tail intensity was used to estimate DNA damage. A total of a hundred randomly selected cells per treatment (two gels per slides) were analyzed using the Comet Assay IV software (Perceptive Instruments, Instem) …

Environmental boron exposure does not induce DNA damage in lymphocytes and buccal cells of females: DNA damage in lymphocytes and buccal cells of boron exposed females
N Başaran, Y Duydu, A Üstündağ, G Taner, SA Dilsiz… - Journal of Trace Elements in …, 2019
… A single scorer randomly selected and captured 100 cells using the Perceptive Instruments COMET Assay IV analysis system at 40x magnification. Tail % intensity was selected as the image analysis parameter. 2.6. Micronucleus assay in buccal cells …

Assessment of induced genomic instability in rat primary astrocytes exposed to intermediate frequency magnetic fields
M Herrala, J Naarala, J Juutilainen - Environmental Research, 2019
… The analysis of 50 (if the number of nuclei was low) or 100 nuclei per slide was performed with a fluorescence microscope (Axio Imager.A1, Carl Zeiss, Göttingen, Germany), using the Comet assay IV (Perceptive Instruments, Haverhill, UK) image analysis...

Absence of cyclin-dependent kinase inhibitor p27 or p18 increases efficiency of iPSC generation without induction of iPSC genomic instability
Z Zhan, L Song, W Zhang, H Gu, H Cheng, Y Zhang… - Cell Death & Disease, 2019
...Each slide was photographed under a Zeiss Axio Observer Z1 microscope and the percentage of tail intensity was computed by the Comet Assay IV software (Perceptive Instruments Ltd.)...

Methamphetamine (“crystal meth”) causes induction of DNA damage and chromosomal aberrations in human derived cells
N Ropek, H Al-Serori, M Mišík, A Nersesyan, HH Sitte… - Food and Chemical Toxicology, 2019
… Air-dried slides were stained with propidium iodide (10 μg/mL) and the %age of DNA in the comet tails was determined with an automated image analysis system (Comet IV, Perceptive Instruments Ltd., Burry St. Edmunds', UK) …

Genetic and epigenetic alterations induced by the small-molecule panobinostat: A mechanistic study at the chromosome and gene levels
MA Al-Hamamah, MR Alotaibi, SF Ahmad, MA Ansari… - DNA Repair, 2019
… Two slides were prepared for each mouse; at least 100 cells were randomly scored for each microscope slide using a Nikon fluorescence microscope and the Comet Assay IV digital imaging software (Perceptive Instruments, Suffolk, UK) …

In vivo systemic toxicity assessment of an oxidized dextrin‐based hydrogel and its effectiveness as a carrier and stabilizer of granular synthetic bone substitutes
I Pereira, S Fraga, L Maltez, J Requicha, L Guardão… - Journal of Biomedical …, 2019
… E400 microscope attached to an epifluorescence illuminator Nikon C-SHG1) with 400xmagnification and the image analysis software Comet Assay IV (Perceptive Instruments, Suffolk, UK). The % DNA in the comet tail (tail intensity) and the olive tail moment ...

The comet assay applied to HepG2 liver spheroids
E Elje, M Hesler, E Rundén-Pran, P Mann, E Mariussen… - Mutation Research/Genetic …, 2019
… visualized after staining with SYBR gold (1:2000, S11494, Sigma-Aldrich), and scored in Leica DMI 6000 B (Leica Microsystems), equipped with a SYBR®photographic filter (Thermo Fischer Scientific) using the software Comet Assay IV

Alkaline comet assay results on fresh and one-year frozen whole blood in small volume without cryo-protection in a group of people with different health status
M Milić, I Ožvald, IV Vrček, MV Lovrenčić, V Oreščanin… - Mutation Research/Genetic …, 2019
...stained with ethidium bromide at a concentration of 20 μg/ml and analysed under the Olympus BX51 (200x magnification) fluorescence microscope using the Comet Assay IV software …

Isolation of leukocytes from frozen buffy coat for comet assay analysis of DNA damage
SK Bøhn, V Vebraite, S Shaposhnikov, AR Collins - Mutation Research/Genetic …, 2019
...the % tail DNA of 50 comets per gel was measured using Comet IV software (Perceptive Instruments, Instem)...

Dosage time affects alkylating agents induced micronuclei in mouse peripheral blood reticulocytes through the function of erythropoietin
K Itoh, S Masumori, D Mukai, H Sakakibara, M Yasuda… - The Journal of toxicological …, 2019
...50 cells per animal were analyzed for their % tail DNA using a Comet Assay IV image …

Assessment of DNA repair efficiency in the inbred BTBR T+tf/J autism spectrum disorder mouse model exposed to gamma rays and treated with JNJ7777120
HA Al-Mazroua, HA Alomar, SF Ahmad, MSA Attia… - Progress in Neuro-Psychopharmacology and Biological Psychiatry, 2019
… Two slides were prepared for each mouse and 150 well-stained bone marrow cells were selected from each slide for evaluation using Comet Assay IV software (Perceptive Instruments, Suffolk, England)…

Lymphocytes exposed to vegetables grown in waters contaminated by anticancer drugs: metabolome alterations and genotoxic risks for human health
C Russo, V Graziani, M Lavorgna, B D'Abrosca… - Mutation Research/Genetic …, 2019
… In each assay, 50 cells per slide (2 slides per each concentrations) were scored using Comet assay IV image analysis software (Perceptive Instruments, UK). The test parameter considered was the Tail intensity (TI, % DNA in tail) …

Protection from DNA Damage by Use of an Aronia Food Supplement—Results from a Pilot Human Intervention Study
T Bakuradze, D Becker, J Reischmann, P Meiser… - Current Pharmacology …, 2019
Comet Assay IV, Perceptive Instruments, Suffolk, GB), scoring 2 × 50 cells per slide. ...

Phorate triggers oxidative stress and mitochondrial dysfunction to enhance micronuclei generation and DNA damage in human lymphocytes
Q Saquib, M Faisal, SM Ansari, R Wahab - Saudi Journal of Biological Sciences, 2019
… ethidium bromide. DNA damage was analyzed on a fluorescence microscope (Nikon Eclipse 80i, Japan) equipped with software (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Biomarkers of DNA damage in COPD patients undergoing pulmonary rehabilitation: Integrating clinical parameters with genomic profiling
P Russo, P Lamonaca, M Milic, E Rojas, G Prinzi… - Mutation Research/Genetic …, 2019
...Scoring and analysis of slides was also performed using the Comet Assay IV analysis system (Perceptive Instruments Ltd., UK), connected with an epifluorescence microscope (Olympus BX51, Tokyo, Japan). A …

Tribocorrosion studies on diamond-like carbon film deposited by PECVD on 304 stainless steel in simulated body fluid
SMM Oliveira, ILM Barzotto, L Vieira, AA Sene, PA Radi… - Biomedical Physics & …, 2019
… and Comet Assay IV image analysis software (Perceptive Instruments, Suffolk, UK). The percentage of DNA in the comet tail (tail intensity %) and the olive tail moment were used as a measure of the amount of DNA damage …

Toxicological assessment of magnesium oxide nanoparticles in HT29 intestinal cells
A Mittag, T Schneider, M Westermann, M Glei - Archives of Toxicology, 2019
… To detect DNA damage, 50 cells in two replicates per experiment were evaluated using the software Comet Assay IV (Perceptive Instruments, UK). DCFH DA assay DCFH-DA can be used to detect oxidatively active sub- stances …

Analysis of the cytotoxic, genotoxic, mutagenic, and pro-oxidant effect of synephrine, a component of thermogenic supplements, in human hepatic cells in vitro
DL Ribeiro, ART Machado, C da Silva Machado… - Toxicology, 2019
...The samples were analyzed using a fluorescence microscope (Carl Zeiss, Axiostar Plus; Jena, Germany) with 515–560 nm excitation filter and 590 nm barrier filter, with the aid of the Comet Assay IV

Upregulation of LGMNP1 confers radiotherapy resistance in glioblastoma
H Xu, B Chen, J Xing, Z Wei, C Liu, Y Qiu, Y Lin, L Ren - Oncology Reports, 2019
… The semi-automated image analysis system Comet Assay IV (Perceptive Instruments Ltd., Bury St. Edmunds, UK) was used to evaluate 100 comets/gel in the case of the H2O2 experiments…

Health indicators and contaminant levels of a critically endangered species in the Gironde estuary, the European sturgeon
ML Acolas, B Davail, P Gonzalez, S Jean… - Environmental Science and …, 2019
… DNA damage was expressed as percentage tail DNA (Hartmann et al. 2003), and 100 randomly selected nucleoids were analyzed on two replicated gels. Image analysis was performed with the Comet 4.0 software (Perceptive instruments Ltd.) …

A non-damaging purification method: decoupling the toxicity of multi-walled carbon nanotubes and their associated metal impurities
C Darne, A Desforges, N Berrada, C Fontana… - Environmental Science …, 2019
..Images of 100 randomly selected comets were acquired and analysed for each sample (Comet assay IV, Perceptive Instruments, UK) in order to evaluate the % tail DNA used as a measure of DNA damage. …

Characterization of urban aerosol: Seasonal variation of genotoxicity of the water-soluble portion of PM2.5 and PM1
C Bocchi - Mutation Research/Genetic Toxicology and …, 2019
… the level of background DNA strand breaks (BSB), was expressed as the percentage of DNA in the tail, compared to the total amount in the whole comet (%DNA in the tail - TI) by a specific image analyser software (Comet Assay IV

Ocean acidification buffers the physiological responses of the king ragworm Alitta virens to the common pollutant copper
C Nielson, C Hird, C Lewis - Aquatic Toxicology, 2019
… One hundred cells per replicate worm were quantified for DNA damage using COMET IV Software (Perceptive Instruments Ltd.), which measures the percentage of DNA present in the comet tail for each cell as the measure …

Physicochemical alterations and toxicity of InP alloyed quantum dots aged in environmental conditions: A safer by design evaluation
A Tarantini, KD Wegner, F Dussert, G Sarret, D Beal… - NanoImpact, 2019
...Comets were scored using image analysis Comet IV software (Perceptive Instruments, Suffolk, UK), and median % DNA in tail was calculated for at least 50 comets per slide.

NEK5 interacts with topoisomerase IIβ and is involved in the DNA damage response induced by etoposide
TD Melo‐Hanchuk, PF Slepicka, AL Pelegrini… - Journal of Cellular Biochemistry
… One hundred randomly selected comets were examined per sample with a Zeiss Axiovert 200 fluorescence microscope (Carl Zeiss, Stuttgart, Germany), under ×400 magnification, using Comet Assay IV software (Perceptive Instruments) …

Impact of Weight Loss Strategies on Obesity Induced DNA Damage
T Setayesh, M Mišík, SAS Langie, R Godschalk… - Molecular nutrition & food …, 2019
...DNA migration was determined with a computer‐aided image analysis system (Comet Assay IV, Perceptive Instruments, Bury St Edmunds, UK). The percentage of DNA in the tail (%DNA in tail) was assessed as a parameter of comet formation…

Effects of UV Radiation on Oxidative Stress in Yellowtail Clownfish Amphiprion clarkii
HS Ryu, CY Choi, JA Song, GS Kil - Ocean Science Journal, 2019
… analysed the percentage of DNA in the tail (tail intensity/total intensity) and tail moment (product of tail length and percentage of DNA in tail), which reflects the amount of DNA damage, using comet assay IV image analysis software …

Boric acid‐induced immunotoxicity and genotoxicity in model insect Galleria mellonella L.(Lepidoptera: Pyralidae)
R Gwokyalya, H Altuntaş - Archives of insect biochemistry and physiology, 2019
… Using the Comet Assay IV imaging software (Perceptive Instruments Ltd, UK‐Italy), comet parameters namely; tail length, tail intensity, tail moment, and tail migration were assessed to determine DNA damage in G. mellonella hemocytes due to BA …

Long-term exposure to a 40-GHz electromagnetic field does not affect genotoxicity or heat shock protein expression in HCE-T or SRA01/04 cells
S Koyama, E Narita, Y Suzuki, T Shiina, M Taki… - Journal of Radiation …, 2019
… After the DNA was stained with SYBR Green I, immunofluorescence images were captured on a fluorescence microscope. DNA strand breaks were analyzed using the Comet software (Perceptive Instruments, Suffolk, UK) …

The comet assay in Ceraeochrysa claveri(Neuroptera: Chrysopidae): A suitable approach for detecting somatic and germ cell genotoxicity induced by agrochemicals
B Gastelbondo-Pastrana, FH Fernandes… - Chemosphere, 2019
… Images from 100 nucleoids (two coded replicates) per insect were blindly scored under 400 x magnification, in a fluorescence microscope connected to an image analysis system (Comet Assay IV Image System, Perceptive Instruments, Haverhill, Suffolk, UK) …

Is the protein profile of pig Longissimus dorsi affected by gender and diet?
G Paredi, F Mori, MG de Marino, S Raboni, L Marchi… - Journal of Proteomics, 2019
… magnification under a Leica DMLS fluorescence microscope (excitation filter BP 515-560 nm, barrier filter LP 580 nm), using an automatic image analysis system (Comet Assay IV—Perceptive Instruments Ltd., Bury St Edmunds, UK) …

Photoautotrophically Grown Chlorella vulgaris Shows Genotoxic Potential but No Apoptotic Effect in Epithelial Cells
A Gille, A Trautmann, M Rodriguez Gomez, SC Bischoff… - Journal of Agricultural and …, 2019
..quantified by the imaging software of Perceptive Instruments (Halstead, UK). The extent of DNA damage is expressed as the percentage of DNA in 166 the comet tail (tail intensity) …

In vivo genotoxicity evaluation of cylindrospermopsin in rats using a combined micronucleus and comet assay
L Díez-Quijada, M Llana-Ruiz-Cabello, GM Cătunescu… - Food and Chemical …, 2019
… Images of at least 150 randomly selected nuclei per animal were analysed with the image analysis software Comet Assay IV (Perceptive Instruments, UK). The % DNA in tail represents DNA strand breaks and oxidized damage in DNA bases …

Integration of Micronucleus, Comet, and Pig-a Gene Mutation Endpoints into Rat 15-day Repeat-treatment Studies: Proof-of-principle with Auramine O
W Tong, C Zhou, P Huang, Y Chang - Mutation Research/Genetic Toxicology and …, 2019
...Comet Assay IV Image Analysis Software (Perceptive Instruments, Haverhill, UK) together with a monochrome video camera and a fluorescence microscope (Olympus model BH-2) was used to score slides…

In vivo genotoxicity evaluation of cylindrospermopsin in rats using a combined micronucleus and comet assay
L Díez-Quijada, M Llana-Ruiz-Cabello, GM Cătunescu… - Food and Chemical …, 2019
… DNA was neutralized in PBS, washed with water and fixed with 70% and absolute ethanol before staining. Images of at least 150 randomly selected nuclei per animal were analysed with the image analysis software Comet Assay IV (Perceptive Instruments, UK) …

Applying the comet assay to fresh vs frozen animal solid tissues: A technical approach
A Azqueta, JM Enciso, L Pastor, AL de Cerain… - Food and Chemical …, 2019
… visualised under a fluorescence microscope (NIKON Eclipse 50 i). DNA damage was quantified in 100 randomly selected comets per slide (50 comets in each gel) by measuring the % tail DNA using the image analysis software Comet Assay IV

In vitro exposure to 2, 2′, 4, 4′-tetrabromodiphenyl ether (PBDE-47) impairs innate inflammatory response
V Longo, A Longo, C Di Sano, D Cigna, F Cibella… - Chemosphere, 2019
… Microscope magnification was set to 5X, the images were acquired and analysed by Comet Assay IV program (Perceptive Instruments, Instem, UK).

Aflatoxin production and in vitro toxicity of Aspergilli section Flavi isolated from air samples collected from different environments
D Jakšić, S Kocsubé, O Bencsik, A Kecskeméti… - Mycotoxin …, 2019
… Before image analysis, DNA was stained for 10 min with ethidium bromide (20 μg/ml). Slides were scored using an image analysis system (Comet assay IV, Instem-Perceptive Instruments Ltd., UK) connected to a fluorescence microscope (Olympus, Japan) …

Shape-engineered titanium dioxide nanoparticles (TiO2-NPs): cytotoxicity and genotoxicity in bronchial epithelial cells
M Gea, S Bonetta, L Iannarelli, AM Giovannozzi… - Food and Chemical …, 2019
… A total of a hundred randomly selected cells per treatment (two gels per slides) were analyzed using the Comet Assay IV software (Perceptive Instruments, Instem)…

Absence of cyclin-dependent kinase inhibitor p27 or p18 increases efficiency of iPSC generation without induction of Ipsc genomic instability
Z Zhan, L Song, W Zhang, H Gu, H Cheng… - Cell death & Disease, 2019
...Each slide was photographed under a Zeiss Axio Observer Z1 microscope and the percentage of tail intensity was computed by the Comet Assay IV software (Perceptive Instruments Ltd.)…

The Polyphenol-Rich Extract from Psiloxylon mauritianum, an Endemic Medicinal Plant from Reunion Island, Inhibits the Early Stages of Dengue and Zika Virus Infection
E Clain, JG Haddad, AC Koishi, L Sinigaglia… - International journal of Moleculat Sciences, 2019
...The pictures were acquired and analyzed using the COMET assay IV software (Instem, Stone, UK)….

Endocytosis and Lack of Cytotoxicity of Alkyl-Capped Silicon Quantum Dots Prepared from Porous Silicon
W Phatvej, HK Datta, SC Wilkinson, E Mutch, AK Daly… - Materials, 2019
... and fluorescent cells images, ×200 magnification (Olympus U-RFLT50/Olympus CX40, Southend-on-Sea, UK) with excitation wavelength 490 nm were counted (50 images for each slide and 2 slides for each treatment). As the glowing cells were counted, the counting capture images were directly connected to the image analysis program (Comet Assay IV, Instem) and the following parameters recorded: head length, tail length, and head intensity of DNA...

Gold-Coated Superparamagnetic Iron Oxide Nanoparticles Attenuate Collagen-Induced Arthritis after Magnetic Targeting
MFH Carneiro, ART Machado, LMG Antunes, TE Souza… - Biological Trace Element …, 2019
...Comets were evaluated as tail intensity (referring to the percentage of DNA in the tail) through Comet Assay IV image analysis software, version 4.3 (Perceptive Instruments Ltd.) …

Dietary hemin promotes colonic preneoplastic lesions and DNA damage but not tumor development in a medium-term model of colon carcinogenesis in rats
NA de Moura, BFR Caetano, LT Bidinotto… - Mutation Research/Genetic …, 2019
… were scored using a Comet Assay IV Image Analysis System (Perceptive Instruments, UK …

Evaluation of DNA damage in haemolymph of freshwater mussels Unio pictorum from Lake Skadar
S Perovic, B Sljukic, M Šrut, A Perovic, GIV Klobučar - Biologia, 2019
… On each slide (per animal) at least 50 cells were examined, and the extent of DNA migration was determined as a percentage of tail DNA (% tDNA) using the image analysis software (Comet assay IV, Perceptive Instruments, UK) …

The Protective Effect of Rosewater against DNA Damages and Oxidant and Antioxidant Parameters of Rats Exposed to Chlorpyrifos Ethyl
Ö Serdal, H ASGARPOUR… - Puerto Rico Health …, 2019
... The measurements of the tail intensity and tail moment of comets were made using a computer-based image analysis system (Comet Assay IV, Perceptive Instruments, UK). The mean value of the percent of tail DNA and the mean tail moment parameters were calculated and used to assess the DNA damage...

In Vitro Mutagenic and Genotoxic Assessment of a Mixture of the Cyanotoxins Microcystin-LR and Cylindrospermopsin
L Díez-Quijada, AI Prieto, M Puerto, Á Jos, AM Cameán - Toxins, 2019
...Olympus BX61 (fluorescence microscope) with the Comet Assay IV software (Perceptive Instruments, UK) available at the Microscopy Service of the University of Seville (CITIUS) was used to score the cells...

Effect of hyperoxia on the immune status of oxygen divers and endurance athletes
F Tillmans, R Sharghi, T Noy, W Kähler… - Free radical …, 2019
…The analysis of the comet assay included four different parameters; tail moment,tail intensity, and tail migration were calculated from Comet Assay IV software from 200 cells nuclei per slide …

Combinatorial Use of Chitosan Nanoparticles, Reversine, and Ionising Radiation on Breast Cancer Cells Associated with Mitosis Deregulation
S Piña Olmos, R Díaz Torres, E Elbakrawy, L Hughes… - Biomolecules, 2019
… The slides were dried at room temperature, protected from light, and scored using the Comet Assay IV software (Perceptive Instruments, Staffordshire, UK) coupled to an epifluorescent microscope (Axio Scope A 1, Zeiss) with 40× objective magnification…

Thymoquinone Enhances the Effect of Gamma Knife in B16-F10 Melanoma Through Inhibition of Phosphorylated STAT3
MA Hatiboglu, A Kocyigit, EM Guler, K Akdur, I Khan… - World neurosurgery, 2019
...A computerized image analysis system (Comet Assay IV [Perceptive Instruments, Staffordshire, United Kingdom]) was used….

TsNTxP, a non-toxic protein from Tityus serrulatus scorpion venom, induces antinociceptive effects by suppressing glutamate release in mice
FK Rigo, GV Bochi, AL Pereira, G Adamante… - European journal of …, 2019
...Two slides were prepared for each sample, and a blind scorer examined 50 randomly selected cells from each slide at 200× magnification. Images were captured and analyzed using Comet Assay IV software (Perceptive Instruments)...

Myristoylated alanine-rich C-kinase substrate effector domain phosphorylation regulates the growth and radiation sensitization of glioblastoma
NJ Eustace, JC Anderson… - International …, 2019
… Tail moments from 200 cells per spot (3X replicates) were determined using a Zeiss AX10 observer A1 microscope and Comet Assay IV version 4.3 software…

Vitamin D supplementation alters the expression of genes associated with hypertension and did not induce DNA damage in rats
CDS Machado, A Ferro Aissa, DL Ribeiro… - Journal of Toxicology …, 2019
...For each sample, a total of 100 nucleoids per animal (two slides of 50 nucleoids each) were evaluated. The comets were scored using the Comet Assay IV software (Perceptive Instruments, Haverhill, England)...

MTH1 inhibitor TH588 induces mitosis-dependent accumulation of genomic 8-oxodG and disturbs mitotic progression
SG Rudd, H Gad, N Amaral, A Hagenkort, P Groth… - bioRxiv, 2019
...DNA was stained with SybrGOLD and comets were quantified with Comet Assay IV software in live video mode…

The sulfite molecule enhances homocysteine toxicity in SH-SY5Y cells
G Gundogdu, Y Dodurga, V Kucukatay - Molecular biology reports, 2019
… DNA damage and any genotoxic effects of hcy, sft and hcy + sft were determined using Comet Assay IV Version 4.3 …

Investigation of comet assays under conditions mimicking ocular instillation administration in a three-dimensional reconstructed human corneal epithelial model
H Tahara, K Sadamoto, Y Yamagiwa… - Cutaneous and …, 2019
...The results of the comet assay (n =3/concentration) were analyzed using the Comet Assay IV 4.3.2 software (Perceptive Instruments, Haverhill, UK). …

Inflammation and (secondary) genotoxicity of Ni and NiO nanoparticles
E Åkerlund, MS Islam, S McCarrick… - …, 2019
...Scoring was blindly performed using a fluorescence microscope (Leica DMLB, Wetzlar, Germany) with the Comet Assay IV software ...

Evaluation of DNA damage in haemolymph of freshwater mussels Unio pictorum from Lake Skadar
S Perovic, B Sljukic, M Šrut, A Perovic, GIV Klobučar - Biologia, 2019
… On each slide (per animal) at least 50 cells were examined, and the extent of DNA migration was determined as a percentage of tail DNA (% tDNA) using the image analysis software (Comet assay IV, Perceptive Instruments, UK) ….

Genotoxic Response and Mortality in Three Marine Copepods Exposed to Waterborne Copper
A Sahlmann, T Lode, J Heuschele, K Borgå, J Titelman… - Toxicology and Chemistry, 2019
…A Sahlmann, T Lode, J Heuschele, K Borgå, J Titelman… - … toxicology and chemistry, 2019 Comets were scored with a Nikon Eclipse LV100ND microscope with LED pE-300 light source (Nikon Corporation, Tokyo, Japan) and Nikon Plan Fluor 20 objective using Comet Assay IV software (version 4.3; Perceptive Instruments, UK) …

Three-dimensional HepaRG spheroids as a liver model to study human genotoxicity in vitro with the single cell gel electrophoresis assay
M Mandon, S Huet, E Dubreil, V Fessard… - Scientific Reports, 2019.
...At least two slides per concentration and 100 cells per slide were analyzed using Comet Assay IV software (Perceptive Instruments, Haverhill, UK). The percentage of DNA in the comet tail (% tail DNA) was used to evaluate the extent of DNA damage. At least three independent experiments were conducted....

Autophagy: A Player in response to Oxidative Stress and DNA Damage
S Galati, C Boni, MC Gerra, M Lazzaretti… - Oxidative Medicine and Cellular Longevity, 2019
… with 75 μl ethidium bromide (10 μg/ml) before the examination at 400x magnification under a Leica DMLS fluorescence microscope (excitation filter BP 515-560 nm and barrier filter LP 580 nm), using an automatic image analysis system (Comet Assay IV, Perceptive Instruments

Loss of genetic integrity in artificially aged seed lots of rice (Oryza sativa L.) and common bean (Phaseolus vulgaris L.)
AF Dantas, ML Fascineli, SCBR José, JG Pádua… - … /Genetic Toxicology and …, 2019
..The evaluation of the nucleoids was done using the Comet Assay IV software, version 4.3.1, and tail intensity was the parameter chosen to measure DNA damage...

Effects of medicinal waters on the UV-sensitivity of human keratinocytes – a comparative pilot study
G Gerencsér, I Szabó, K Szendi, A Hanzel… - International journal of Biometeorology…, 2019
...Fifty nuclei were analysed on each slide using Comet Assay IV software (Perceptive Instruments Ltd., 400X). The degree of DNA damage is termed as tail moment (TM) and is calculated by multiplying the tail length by the percentage of DNA in the tail…

Genomic Integrity Safeguards Self-Renewal in Embryonic Stem Cells
J Su, D Zhu, Z Huo, JA Gingold, YS Ang, J Tu, R Zhou… - Cell Reports, 2019
...Images were captured using MetaVue Imaging (Molecular Devices). The comet tail was measured by Comet Assay IV software (Perceptive Instruments)...

Organophosphorus flame retardant (tricresyl phosphate) trigger apoptosis in HepG2 cells: Transcriptomic evidence on activation of human cancer pathways
AM Al-Salem, Q Saquib, MA Siddiqui, J Ahmad… - Chemosphere, 2019
...DNA damage in cells was quantitated using fluorescence microscope (Nikon Eclipse 80i, Japan) equipped with software (Comet Assay IV, Perceptive Instruments, UK)…

From DNA to ecological performance: Effects of anthropogenic noise on a reef-building mussel
MA Wale, RA Briers, MGJ Hartl, D Bryson… - Science of The Total Environment, 2019
...Prepared slides were viewed under an epifluorescence microscope (Zeiss Axioplan, Carl Zeiss Microscopy, Oberkochen, Germany), using Comet Assay IV software (Perceptive Instruments, Bury Saint Edmunds, UK). DNA damage is expressed as % tail DNA …

Adverse Effects Induced by Chronic Gamma Irradiation in Progeny of Adult Fish Not Affecting Parental Reproductive Performance
N Guirandy, B Gagnaire, S Frelon, T Munch… - … Toxicology and Chemistry, 2019
… Two hundred nucleoids per slide were analysed at ×400 magnification under a fluorescence microscope (Nikon Eclipse E600) equipped with a 515–560 nm excitation filter. Comet pictures were analysed using Comet IV software (Perceptive Instruments)…

Hyssopus officinalis subsp. aristatus: An unexploited wild-growing crop for new disclosed bioactives
F Borrelli, E Pagano, C Formisano, S Piccolella… - Industrial Crops and Products, 2019
...Images were acquired using a Leica DCF 340 FX microscope equipped with Comet Assay™ software (Perceptive Instruments, UK)…

Gamma radiation induces life stage-dependent reprotoxicity in Caenorhabditis elegans via impairment of spermatogenesis
E Maremonti, DM Eide, DH Oughton, B Salbu… - Science of The Total …, 2019 - Elsevier
...Forty randomly chosen cells per replicate (160 cells per biological replicate, total of 480 cells per dose rate) were scored using the Comet IV analysis software (Perceptive Instruments Ltd., Bury St. Edmunds, UK). Tail intensity (% Tail DNA), defined as the percentage of DNA migrated from the head of the comet into the tail, was used as a measure of DNA damage induced by gamma radiation...

Increased levels of genotoxic damage in a Bolivian agricultural population exposed to mixtures of pesticides
JB Cuenca, N Tirado, J Barral, I Ali, M Levi… - Science of The Total Environment, 2019
...100 comets were scored per sample using a fluorescence microscope (Leica DMLB) and Comet assay IV software (Perceptive Instruments). ...

Evaluation of Cytotoxicity and Genotoxicity Induced by Oleic Acid‐Coated Iron Oxide Nanoparticles in Human Astrocytes
N Fernández‐Bertólez, C Costa… - Environmental and Molecular Mutagenesis, 2019
... In all cases, fifty randomly selected comets per replica (ie 100 cells in total) were scored, and the percentage of DNA in the comet tail (%tDNA), used as DNA damage parameter, was analyzed using the Comet IV Software (Perceptive Instruments)…

Regulation of response to radiotherapy by β-arrestin1 in Non-small cell lung cancer
L Wang, K Wang, W Dong, H Shen, J Du - Journal of Cancer, 2019
...Cells were stained with Vista Green DNA dye and visualized by the epifluorescence microscopy. Tail length, head length, and tail moment were analyzed by COMET-Assay IV software V4.3 (Perceptive Instruments, UK)...

High variability in toxicity of welding fume nanoparticles from stainless steel in lung cells and reporter cell lines: the role of particle reactivity and solubility
S McCarrick, Z Wei, N Moelijker, R Derr… - …, 2019
...Following electrophoresis, slides were neutralized, dried, and stained with 1:10 000 dilution of Sybr Green in TAE buffer for 15 min and further scored using a fluorescence microscope (Leica DMLB, Germany) with Comet assay IV software (Perceptive Instruments, Suffolk, UK). At least 50 cells were scored per sample in duplicates...