Papers from 2019

Spatial and temporal variation of three biomarkers in Mytilus edulis
S Strubbia, BP Lyons, RJ Lee - Marine Pollution Bulletin, 2019
...The tails were evaluated through Comet Assay IV analysis software (Perceptive Instruments Ltd) and Camera AVT smart view for, Fire Package version 1.6.2) to measure the total image fluorescence intensity, head intensity, tail intensity, head length and tail length. The results were recorded as the % tail intensity over 50 cells...

Poly (e-caprolactone) microspheres for prolonged release of selenium nanoparticles
N Filipovic, L Veselinovic, S Razic, S Jeremic, M Filipic… - Materials Science and Engineering, 2019
... Images of 50 randomly selected nuclei per experimental point were analyzed with image analysis software Comet Assay IV (Perceptive Instruments, UK). The percentages of tail DNA were used to measure the levels of DNA damage …

Multivitamin and Mineral Supplementation Containing Phytonutrients Scavenges Reactive Oxygen Species in Healthy Subjects: A Randomized, Double-Blinded, Placebo-Controlled Trial
S Kang, Y Lim, Y Kim, E Jung, D Suh, C Lee, E Park… - Nutrients, 2019
… Comet assay was performed using a single-cell gel electrophoresis assay as previously described [23] … Images from 50 comets on each slide were analyzed with COMET VI image analysis software (Perceptive Instruments, Suffolk, UK) …

Application of Crayfish Astacus Leptodactylus for the Analysis of Water Genotoxicity in the Lake Sevan Basin
AE Simonyan, AA Sargsyan, GG Hovhannisyan… - Journal of Water Chemistry and TEchnology, 2018
… microscope (ZEISS, Germany). The estimation of DNA damages was performed by using commercial software Comet Assay VI (Perspective Instruments, UK). 150 cells were analyzed for each crayfish. The statistical processing …

Cytotoxic, genotoxic, and oxidative stress-inducing effect of an l-amino acid oxidase isolated from Bothrops jararacussu venom in a co-culture model of HepG2 and HUVEC cells
ART Machado, AF Aissa, DL Ribeiro, TR Costa… - International Journal of Biological Macromolecules, 2019
… One hundred nucleoids per sample were analyzed with the aid of the Comet Assay IV™ software (Perceptive Instruments, Haverhill, UK). Percentage (%) of DNA in the tail (Tail Intensity) was used as a parameter of DNA damage …

Efficient Single-Strand Break Repair Requires Binding to Both Poly (ADP-Ribose) and DNA by the Central BRCT Domain of XRCC1
LM Polo, Y Xu, P Hornyak, F Garces, Z Zeng… - Cell Reports, 2019
Finally, DNA was stained with SybrGreen I (1:10,000 in PBS) for 30 min. Average tail moments from 100 cells/sample were measured using Comet Assay IV software (Perceptive Instruments, UK). Data are the average ± 1?SEM of three independent experiments and were scored blind.

The CHD6 chromatin remodeler is an oxidative DNA damage response factor
S Moore, ND Berger, MS Luijsterburg, CG Piett… - Nature Communications, 2019
Comet scoring was performed on a Zeiss Axiovision microscope with a camera associated with Comet Assay IV software (Perspective Instruments). A total of 100 comets were scored per condition, per repeat the data expressed as the average comet tail moment on a scatter plot.

TA new endodontic mixture based on calcium aluminate cement obtained by hydrothermal synthesis
SB Colovic, O Jankovic, S Živkovic, Ž Žižak, IB Žižak… - Ceramics International, 2019
For statistical analysis of the results of comet assay, Comet assay IV software (Perceptive Instruments) was used

Oxygen Availability during Growth Modulates the Phytochemical Profile and the Chemo-Protective Properties of Spinach Juice
F Milano, F Mussi, S Fornaciari, M Altunoz, L Forti… - Biomolecules, 2019
Slides were examined at 400× magnification under a Leica DMLS fluorescence microscope (excitation filter BP 515–560 nm, barrier filter LP 580 nm), using an automatic image analysis system (Comet Assay III

The WD40 domain of FBXW7 is a poly (ADP-ribose)-binding domain that mediates the early DNA damage response
Q Zhang, ASA Mady, Y Ma, C Ryan, TS Lawrence… - Nucleic Acids Research, 2019
Then, the slides were subjected to electrophoresis at 20 V (0.6 V/cm) for 30 min and stained in 2.5 µg/ml propidium iodide for 20 min. All images were taken with a fluorescence microscope and analyzed by Comet Assay IV software (Perceptive Instruments).

miR-205 enhances radiation sensitivity of prostate cancer cells by impairing DNA damage repair through PKCe and ZEB1 inhibition
R El Bezawy, S Tinelli, M Tortoreto, V Doldi, V Zuco… - Journal of Experimental & Clinical Cancer Research…, 2019
… Comets were imaged using a fluorescence microscope equipped with a video camera (Jai Pulnix, Sunnyvale, CA), and quantitative assessment of DNA damage was obtained using the Comet Assay IV software (Perceptive Instruments, Suffolk, UK) …

The comet assay for human biomonitoring: Effect of cryopreservation on DNA damage in different blood cell preparations
C Ladeira, G Koppen, F Scavone, L Giovannelli - Mutation Research/Genetic …, 2019
… distribution graphs were performed and generated with Comet Assay IV® software (Perceptive Instruments) … different blood preparation for use in biomonitoring with the comet assay, with particular … The latter have been …