Papers from 2019

Spatial and temporal variation of three biomarkers in Mytilus edulis
S Strubbia, BP Lyons, RJ Lee - Marine Pollution Bulletin, 2019
...The tails were evaluated through Comet Assay IV analysis software (Perceptive Instruments Ltd) and Camera AVT smart view for, Fire Package version 1.6.2) to measure the total image fluorescence intensity, head intensity, tail intensity, head length and tail length. The results were recorded as the % tail intensity over 50 cells...

Poly (e-caprolactone) microspheres for prolonged release of selenium nanoparticles
N Filipovic, L Veselinovic, S Razic, S Jeremic, M Filipic… - Materials Science and Engineering, 2019
... Images of 50 randomly selected nuclei per experimental point were analyzed with image analysis software Comet Assay IV (Perceptive Instruments, UK). The percentages of tail DNA were used to measure the levels of DNA damage …

Multivitamin and Mineral Supplementation Containing Phytonutrients Scavenges Reactive Oxygen Species in Healthy Subjects: A Randomized, Double-Blinded, Placebo-Controlled Trial
S Kang, Y Lim, Y Kim, E Jung, D Suh, C Lee, E Park… - Nutrients, 2019
… Comet assay was performed using a single-cell gel electrophoresis assay as previously described [23] … Images from 50 comets on each slide were analyzed with COMET VI image analysis software (Perceptive Instruments, Suffolk, UK) …

Application of Crayfish Astacus Leptodactylus for the Analysis of Water Genotoxicity in the Lake Sevan Basin
AE Simonyan, AA Sargsyan, GG Hovhannisyan… - Journal of Water Chemistry and TEchnology, 2018
… microscope (ZEISS, Germany). The estimation of DNA damages was performed by using commercial software Comet Assay VI (Perspective Instruments, UK). 150 cells were analyzed for each crayfish. The statistical processing …

Cytotoxic, genotoxic, and oxidative stress-inducing effect of an l-amino acid oxidase isolated from Bothrops jararacussu venom in a co-culture model of HepG2 and HUVEC cells
ART Machado, AF Aissa, DL Ribeiro, TR Costa… - International Journal of Biological Macromolecules, 2019
… One hundred nucleoids per sample were analyzed with the aid of the Comet Assay IV™ software (Perceptive Instruments, Haverhill, UK). Percentage (%) of DNA in the tail (Tail Intensity) was used as a parameter of DNA damage …

Efficient Single-Strand Break Repair Requires Binding to Both Poly (ADP-Ribose) and DNA by the Central BRCT Domain of XRCC1
LM Polo, Y Xu, P Hornyak, F Garces, Z Zeng… - Cell Reports, 2019
Finally, DNA was stained with SybrGreen I (1:10,000 in PBS) for 30 min. Average tail moments from 100 cells/sample were measured using Comet Assay IV software (Perceptive Instruments, UK). Data are the average ± 1?SEM of three independent experiments and were scored blind.

The CHD6 chromatin remodeler is an oxidative DNA damage response factor
S Moore, ND Berger, MS Luijsterburg, CG Piett… - Nature Communications, 2019
Comet scoring was performed on a Zeiss Axiovision microscope with a camera associated with Comet Assay IV software (Perspective Instruments). A total of 100 comets were scored per condition, per repeat the data expressed as the average comet tail moment on a scatter plot.