Papers from 2014

Unbiased compound screening identifies unexpected drug sensitivities and novel treatment options for gastrointestinal stromal tumors
S Boichuk, DJ Lee, KR Mehalek, KR Makielski… - Cancer Research, 2014
... DNA was labeled with SYBR Green and slides were viewed under a Nikon ECLIPSE 50i epifluorescence microscope (Nikon). Comet tails were analyzed using Comet Assay IV v4.2 software (Perceptive Instruments). At least 100 cells were analyzed per sample. ...

Is the antioxidative effectiveness of a bilberry extract influenced by encapsulation?
M Baum, M Schantz, S Leick, S Berg, M Betz, K Frank… - … of the Science of Food and …, 2014
... Slides were analysed by computerized image analysis (Comet IV, Perceptive Instruments. DNA damage was calculated as the tail intensity (TI%: DNA in the comet tail as a percent of total DNA). ... 20. Schantz M, Baum M and Richling E, Antioxidative efficiency of an anthocyanin ...

Effects of Prochloraz on Dna Damage, Lipid Peroxidation and Antioxidant System in vitro
B Alpertunga, M Kara, M Abudayyak, E Oztas, S Ozden… - Toxicology Mechanisms and …, 2014
... 7 (40x10) magnification by using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). 100 cells were scored per concentration and DNA damage to individual cells was expressed as a percentage of DNA in the comet tail (%TDNA, tail ...

Toxicity Assessment of Silica Coated Iron Oxide Nanoparticles and Biocompatibility Improvement by Surface Engineering
MA Malvindi, V De Matteis, A Galeone, V Brunetti… - PLOS ONE, 2014
... hour. Comets derived from single cells were photographed under a Leica fluorescence microscope and head and tail DNA intensity of each comet were quantified using Comet IV program (Perceptive Instruments). Differences ...

Assessment of the reproductive toxicity of inhalation exposure to ethyl tertiary butyl ether in male mice with normal, low active and inactive ALDH2
Z Weng, K Ohtani, M Suda, Y Yanagiba, T Kawamoto… - Archives of Toxicology, 2014
... allowed to air-dry. After staining slides with 50 µl SyBR green 1(Trevigen, gaithersburg, MD), 100 cells were scored per sample using the Comet Assay IV capture system (Perceptive Instruments, Suffolk, uK). The tail intensity, defined ...

Lack of genotoxic potential of ZnO nanoparticles in in vitro and in vivo tests
JY Kwon, SY Lee, P Koedrith, JY Lee, KM Kim, JM Oh… - Mutation Research/Genetic …, 2014
... All slides were independently coded before the microscopic analysis. The comet was observed via fluorescence microscope (Nikon, Japan) at magnification of 200X and analysed by COMET ASSAY IV software (Perceptive instruments, UK). ...

Absence of in vitro genotoxicity potential of the mycotoxin deoxynivalenol in bacteria and in human TK6 and HepaRG cell lines
N Takakura, F Nesslany, V Fessard, LLE Hegarat - Food and Chemical Toxicology, 2014
... Fifty to one hundred cells per slide and 2 slides per concentration were analysed using the Comet Assay IV software (Perceptive Instruments, Haverhill, UK). The extent of DNA damage in individual cells was evaluated by the percentage of tail DNA. ...

Performance of Comet and Micronucleus Assays in Metabolic Competent Heparg Cells to Predict In Vivo Genotoxicity
L Le Hegarat, A Mourot, S Huet, L Vasseur… - Toxicological …, 2014 - Soc Toxicology
... Comet Assay IV software (Perceptive Instruments, Haverhill, UK). The extent of DNA ... compared the performance of the comet assay and the micronucleus test using the metabolic ... suitable model for detecting direct mutagens and pro-carcinogens with comet assay and MN ...

Kinetic response of a genotoxicity biomarker in the three-spined stickleback and implication for environmental monitoring
S Raphael, PL Mélissa, B Aurélien, V Emmanuelle… - … and Environmental Safety, 2014
... Second, the visual classification was calibrated through the comparison with the Comet IV software (Perceptive Instruments, Ltd), enabling to assign each class of the visual scoring to a tail intensity value (Fig.1). Finally categories (from 0 to 4) were assigned to comets by the ...

Responses of the European flounder (Platichthys flesus) to a mixture of PAHs and PCBs in experimental conditions
C Dupuy, C Galland, A Devaux, S Bony, V Loizeau… - Environmental Science and …, 2014
... (2010a). After cell lysis and electrophoresis, DNA was stained with 0.05 mM ethidium bromide and scored using an Axioskop epi-fluorescence microscope (Zeiss™) and the Comet assay IV image analysis system (Perceptive Instruments Ltd., Haverhill, UK). ...

In vitro and in vivo genotoxicity assessment of HI-6 dimethanesulfonate/oxime
L Nakab, I Bardot, S Bardot, S Simar, D Marzin… - Mutation Research/Genetic …, 2014
... of 515-560 nm and a barrier filter of 590 nm, connected via a gated monochrome CCD IEEE1394 FireWire video camera (Allied Vision Technologies), to the Comet Assay IV Image Analysis System, version 4.11 with Windows XP Pro Software (Perceptive Instruments Ltd, Suffolk ...

Genome stability in the uvh6 mutant of Arabidopsis thaliana
A Bilichak, Y Yao, V Titov, A Golubov, I Kovalchuk - Plant Cell Reports
... Vision Technologies). Comets were evaluated with the Comet Assay IV software system (Perceptive Instruments Ltd.) using single click automatic scoring. The Olive tale moment values were used to measure DNA damage. The ...

Different toxic mechanisms are activated by emission PM depending on combustion efficiency
O Uski, PI Jalava, MS Happo, J Leskinen, O Sippula… - Atmospheric Environment, 2014
... described by Jalava et al. (2010b). The nuclei were analyzed in a blinded manner (100 nuclei per concentration) using an image analysis system (Comet assay IV, Perceptive Instruments Ltd., Suffolk, UK). The Olive tail moment ([(tail ...

Is organic farming safer to farmers health? A comparison between organic and traditional farming
C Costa, J García-Lestón, S Costa, P Coelho, S Silva… - Toxicology Letters, 2014
... Image capture and analysis were performed with Comet Assay IV software (Perceptive Instruments); percentage of tail DNA (%T) was the DNA damage parameter evaluated according to what has been recommended by Kumaravel et al. (2009). ...

In vivo assessment of the cytotoxic, genotoxic and antigenotoxic potential of maná-cubiu (Solanum sessiliflorum Dunal) fruit
LC Hernandes, AF Aissa, MR de Almeida, J Darin… - Food Research International, 2014
... Axiostarplus). Single cells were analyzed by the CometAssay IV software (Perceptive Instruments Ltd., Suffolk, United Kingdom) using the parameter Tail Moment to measure DNA damage. 2.6. Micronucleus Test. The micronucleus ...

Expression of 8-oxoguanine Glycosylase in Human Fetal Membranes
R Menon, J Polettini, TA Syed, GR Saade, I Boldogh - American Journal of …, 2014
... at 37°C. Electrophoresis was conducted for 30 min at 21 V. Fifty cells per culture were counted under an Olympus microscope (40× objectives), and scoring of the comet tail DNA content was performed using the Comet Assay IV v4.2 system (Perceptive Instruments, Suffolk, UK). ...

Mechanistic Evaluation of Ginkgo Biloba Leaf Extract-Induced Genotoxicity in L5178Y Cells
H Lin, X Guo, S Zhang, SL Dial, L Guo, MG Manjanatha… - Toxicological Sciences, 2014
... The slides were scored using an imaging analysis system consisting of a Nikon 501 fluorescent microscope (Nikon Instruments Inc.; Melville, NY) and CometAssay IV digital analysis system (Perceptive Instruments Ltd.; Bury St. Edmunds, UK). The percent DNA in the ...

Developmental toxicity of PAH mixtures in fish early life stages. Part II: adverse effects in Japanese medaka
F Le Bihanic, C Clérandeau, K Le Menach… - … Science and Pollution …, 2014 - Springer
... DNA damage was measured at 100 nuclei per sample using Comet Assay IV (Perceptive instruments, Bury St Edmunds, UK) using ... Developmental effects ... inflation in larvae at hatching (Table 2). The two oil fractions did not affect the kinetic of embryo development, although 3 ...

Low Dose of the Anesthetic Propofol Does Not Induce Genotoxic or Mutagenic Effects in Nile Tilapia
G Valença-Silva, MG Braz, RE Barreto, DMF Salvadori… - Transactions of the …, 2014
... Images from 50 nucleoids (duplicate) per fish were scored using the Comet Assay IV Image System (Perceptive Instruments, Haverhill, Suffolk, UK). Tail intensity (percent DNA in tail) was used to estimate the extent of DNA damage. Data table, ...

Both genetic and dietary factors underlie individual differences in DNA damage levels and DNA repair capacity
J Slyskova, Y Lorenzo, A Karlsen, MH Carlsen… - DNA Repair, 2014
... sites) were measured using the comet assay [40]. Comet Assay IV software (Perceptive Instruments) was used to evaluate % tail DNA of 50 comets per duplicate gel. Each experiment included negative control (NC; PBMC + buffer ...

Synthesis of poly (E-caprolactone) nanospheres in the presence of the protective agent poly (glutamic acid) and their cytotoxicity, genotoxicity and ability to induce …
N Filipovic, M Stevanovic, J Nunic, S Cundric, M Filipic… - Colloids and Surfaces B: …, 2014
... with minor modifications [35]. Images of 50 randomly selected nuclei per experimental point were analyzed using the image analysis software Comet Assay IV (Perceptive Instruments, UK). Three independent experiments were performed for each of the treatment conditions. ...

CELL CYCLE KINETICS, APOPTOSIS RATES, DNA DAMAGE AND TP53 GENE EXPRESSION IN BLADDER CANCER CELLS TREATED WITH ALLYL …
ALV Sávio, GN Silva, EA Camargo, DMF Salvadori - Mutation Research/Fundamental …, 2014
... A total of 150 randomly selected nucleoids per treatment was analyzed under 400x magnification with a fluorescence microscope connected to an image analysis system (Comet Assay IV, Perceptive Instruments; Suffolk, Haverhill, UK). ...

Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells
V Selvaraj, S Bodapati, E Murray, KM Rice, N Winston… - International Journal of …, 2014
... A total of 100 cells were scored for each Y2O3 NP concentration in duplicate on three different experi- ments. Images were analyzed using the Perceptive Comet IV assay software version 4.3 Lite, analysis system (Perceptive Instruments Ltd, Bury St Edmunds, Suffolk, UK). ...

Isoflavones in aglycone solution enhance ultraviolet B-induced DNA damage repair efficiency
B Iovine, M Garofalo, M Orefice, V Giannini, F Gasparri… - Clinical and Experimental …, 2014
... The TM is defined as the product of the tail length and the fraction of total DNA in the tail (%tail DNA × tail length). The data analysis was carried out using Comet Score software (Comet Assay IV, Perceptive Instruments, Blois Meadow, Suffolk, UK). ... ...

Genotoxic effects of Ag2S and CdS nanoparticles in blue mussel (Mytilus edulis) haemocytes
M Munari, J Sturve, G Frenzilli, MB Sanders… - Chemistry and Ecology, 2014
... buffer (0.4 M Tris; pH 7.5). Air-dried slides were stained with ethidium bromide (2 µg/mL in ) and scored using Comet Assay IV (Perceptive Instruments Ltd). 50 cells for each half slide have been scored. 100 cells were scored per ...

Variability in DNA damage of chub (Squalius cephalus L.) blood, gill and liver cells during the annual cycle
K Sunjog, S Kolarevic, M Kracun-Kolarevic, Z Gacic… - Environmental Toxicology …, 2014
... magnifications, 510-560 nm excitation filter, and 590 nm barrier filter). Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). Images of 50 cells were captured from each slide per ...

Cytolethal distending toxin (CDT) is a radiomimetic agent and induces persistent levels of DNA double-strand breaks in human fibroblasts
J Fahrer, J Huelsenbeck, H Jaurich, B Dörsam, T Frisan… - DNA Repair, 2014
... Comets were analyzed by microscopy using an Olympus BX50 equipped with a ColorView camera (Olympus, Münster, Germany). In each experiment, 50 cells were scored using Comet IV software (Perceptive Instruments Ltd., Bury St Edmunds, UK). ...

Investigation of the in vitro toxicological properties of the synthetic cannabimimetic drug CP-47,497-C8
VJ Koller, V Auwärter, T Grummt, B Moosmann, M Mišík… - Toxicology and Applied …, 2014
... shown). Air-dried slides were stained with ethidium bromide (20 µg/ml) and the percentage of DNA in tail measured by use of a computer aided image analysis system (Comet IV, Perceptive Instruments Ltd., Haverhill, UK). In ...

Genotoxic and cytotoxic effects of doxorubicin and silymarin on human hepatocellular carcinoma cells
E Yurtcu, ÖD Iseri, FI Sahin - Human & experimental toxicology, 2014
... After neutralization (0.4 M Tris; pH 7.5), the slides were stained with 2 µg/mL EtBr and observed under fluorescence microscope (Nikon, Eclipse 600, Japan). A computerized image analysis system (comet assay IV; Perceptive Instruments, Bury St Edmunds, UK) was employed. ...

Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach
S Triboulet, C Aude-Garcia, L Armand, H Diemer… - Nanoscale, 2014
... fluorescence microscope (Zeiss) equipped with a 350-390 nm excitation and a 456 nm emission filter at ×20 magnification. Comet length and intensity were measured by using Comet IV software (Perceptive Instruments, Suffolk, UK). Proteomics Page 16 of 39 Nanoscale ...

Comparison of plasma effects on human keratinocytes using different plasma treatment regimes
S Strassenburg, U Greim, R Bussiahn, B Haertel… - Plasma Medicine
... (Olympus CK 40, Hamburg, Germany) connected to a camera (Infinity 3, Lumenera, Ottawa, Canada). Nucleoids were analyzed with Comet Assay IV software (Perceptive Instruments, Suffolk, United Kingdom). Tail Intensity (percentage of DNA in the tail) is the parameter ...

Redox and epigenetic regulation of theAPE1 gene in the hippocampus of piglets: The effect of early life exposures
SAS Langie, P Kowalczyk, B Tomaszewski, A Vasilaki… - DNA Repair, 2014
... added. A total of 50 comets/slide selected at random were analysed using the Comet assay IV software program (Perceptive Instruments, Haverhill, UK). %DNA in the tail (aka tail intensity (TI)) was used for further calculations. ...

Micronucleus, alkaline, and human 8-oxoguanine glycosylase 1 modified comet assays evaluation of glass-ionomer cements-in vitro
E Galic, A Tadin, N Galic, V Kašuba, M Mladinic… - Archives of Industrial …, 2014
... slide. The edges of the gel, occasional dead cells, and superimposed comets were avoided. Tail length and tail intensity were measured using Comet Assay IV analysis system (Perceptive Instruments Ltd., Suffolk, UK). Oxidative ...

The low molecular weight DNA diffusion assay as an indicator of cytotoxicity for the in vitro comet assay
G Speit, A Vesely, P Schütz, R Linsenmeyer… - Mutagenesis, 2014
... Images of 100 randomly selected cells were analysed from one slide per culture. Measurements were made by image analysis (Comet Assay IV, Perceptive Instruments, Haverhill, UK) and DNA migration was determined by measuring the 'tail intensity' (% tail DNA). ...

Effects of depleted uranium on the reproductive success and F1 generation survival of zebrafish ( Danio rerio)
S Bourrachot, F Brion, S Pereira, M Floriani, V Camilleri… - Aquatic Toxicology, 2014
... Comet figures were analyzed using Comet IV software (Perceptive Instruments, Suffolk, UK) and DNA damage was quantified as tail intensity values. 2.6. Statistical analysis. All analyses were performed using Statistica 7.1 software (Statsoft, 2005). ...

The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum …
Z Gacic, S Kolarevic, K Sunjog, M Kracun-Kolarevic… - Environmental Pollution, 2014
... The slides were examined with a fluorescence microscope (Leica, DMLS, Austria, under magnification 400×, excitation filter 510–560 nm, barrier filter 590 nm). Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). ...

The impact of gypsum mine water: A case study on morphology and DNA integrity in the freshwater invertebrate, Gammarus balcanicus
I Ternjej, Z Mihaljevic, M Ivkovic, A Previšic… - Environmental …, 2014 - Elsevier
... Results of the water and sediment analyses are presented in Table 1. Basic ... Photomicrographs have been acquired by image analysis system Comet Assay IV (Perceptive Instruments) under magnification ... that all of the differences between parameters of comet assay evaluated ...

Synthesis of poly (e-caprolactone) nanospheres in the presence of the protective agent poly (glutamic acid) and their cytotoxicity, genotoxicity and ability to induce oxidative stress …
N Filipovic, M Stevanovic, J Nunic, S Cundric… - Colloids and Surfaces B: …, 2014 - Elsevier
... nuclei per experimental point were analyzed using the image analysis software Comet Assay IV (Perceptive Instruments ... and the drying time), (iii) the method of solidification or (iv) the combination ... measured the kinetics of their formation in HepG2 cells using a DCFH-DA assay. ...

Anti-oxidative effects and harmlessness of asymmetric Au@ Fe3O4 Janus particles on human blood cells
L Landgraf, P Ernst, I Schick, O Köhler, H Oehring… - Biomaterials, 2014
... The fluorescence intensities of head and tail were measured by considering the nuclei of a triplicate of 20 cells in different fields of view via the comet assay software (Comet Assay IV, Perceptive Instruments Ltd, Bury St Edmunds, UK). ...

Protective effect of boric acid on lead-and cadmium-induced genotoxicity in V79 cells
A Üstündag, C Behm, W Föllmann, Y Duydu… - Archives of …, 2014 - Springer
... were examined on a fluorescence microscope (Leica DM 1000) with the Comet Assay IV Software. ...Figure 4 presents the results of the comet assay, comparing tail intensity values for the ... in humanwhole blood cultures, BA at 2.5 ppm (or 40 µM) had a stronger protective effect ...

Mouse Spermatocytes Express CYP2E1 and Respond to Acrylamide Exposure.
Nixon BJ, Katen AL, Stanger SJ, Schjenken JE, Nixon B, Roman SD.PLoS One. 2014 May 2;9(5):
... visualised under fluorescence. The DNA integrity of 50–100 cells per slide was analysed using Comet Assay IV software (Perceptive Instruments, Suffolk, UK). The fluorescence intensity in the comet “tail” was used as ...

Agents of earthy-musty taste and odor in water: Evaluation of cytotoxicity, genotoxicity and toxicogenomics
L Burgos, M Lehmann, D Simon, HHR de Andrade… - Science of The Total …, 2014
... The stained nucleoids were immediately evaluated at 400 × magnification under an Olympus (BX41) fluorescence microscope (excitation filter, 515–560 nm; barrier filter, 590 nm), equipped with an image analysis system (Comet Assay IV, Perceptive Instruments, UK). ...

Methanolic extract ofHypericum perforatum cells elicited with Agrobacterium tumefaciens provides protection against oxidative stress induced in …
AC Carvalho, G Franklin, ACP Dias, CF Lima - Industrial Crops and Products, 2014
... After electrophoresis, the slides were neutralized (0.4 M Tris, pH 7.5), stained with SYBR Gold nucleic acid gel stain, and analyzed by fluorescence microscopy, using the Comet Assay IV 4.3 software (Perceptive Instruments Ltd., Suffolk, UK). ...

Genotoxic potential and in-vitro tumour-promoting potential of 2-dodecylcyclobutanone and 2-tetradecylcyclobutanone, two radiolytic products of fatty acids
K Yamakage, H Sui, R Ohta, T Toyoizumi, K Kawakami… - Mutation Research/Genetic …, 2014
... dried. One hundred cells (50 cells/dish) were analyzed, after staining with SYBR Gold solution, by means of an image-analyzer system (Comet Assay IV version no. 4.11; Perceptive Instruments, Suffolk, UK). The percentage ...

Antigenotoxic and antioxidant effects of the mongolian medicinal plantLeptopyrum fumarioides(L): An in vitro study
D Boldbaatar, HR El-Seedi, M Findakly, S Jabri… - Journal of …, 2014 - Elsevier
and the software Comet Assay IV (Perspective Instruments ...Apparently dead cells (comets without distinct heads: “clouds”) and superimposed comets ...

Using a medium-throughput comet assay to evaluate the global DNA methylation status of single cells
A Lewies, E van Dyk, JF Wentzel, PJ Pretorius - Name: Frontiers in Genetics, 2014
... distilled water. The comet images were captured with 146 an Olympus IX70 fluorescence microscope (200x magnification) and scored using Comet IV computer software version 4.3.1 (Perceptive Instruments Ltd). At least ...

Concurrent evaluation of general, immune, and genetic toxicity endpoints as part of an integrated testing strategy
MR Schisler, R Sura, NR Visconti, LK Sosinski… - … and Molecular Mutagenesis, 2014
... One hundred randomly selected cells were analyzed per sample for the extent of DNA damage expressed as a percent of DNA in the tail using Comet Assay IV software (Perceptive Instruments, Haverhill, UK). Statistics. Body ...

ATM regulates NF-?B-dependent immediate-early genes via RelA Ser 276 phosphorylation coupled to CDK9 promoter recruitment
L Fang, S Choudhary, Y Zhao, CB Edeh, C Yang… - Nucleic Acids Research, 2014
... Comets were analyzed by using the Comet Assay IV v4.2 system (Perceptive Instruments, Suffolk, UK) image analysis software (4). The fold change in tail comet moments are expressed as the mean (± SD) from three parallel experiments. ...

Evaluation of single-cell gel electrophoresis data: combination of variance analysis with sum of ranking differences
K Héberger, S Kolarevic, M Kracun-Kolarevic, K Sunjog… - Mutation Research/Genetic …, 2014
... The slides were examined with a fluorescence microscope (Leica, DMLS, Austria, magnification 400×, excitation filter 510-560 nm, barrier filter 590 nm). Microscope images of comets were scored with Comet IV Computer Software (Perceptive Instruments, UK). ...

Novel amphiphilic cationic porphyrin and its Ag (II) complex as potential anticancer agents
A Tovmasyan, N Babayan, D Pogosyan, K Margaryan… - Journal of Inorganic …, 2014
... Image analysis software Comet 4 (Perceptive instruments, UK) was used to analyze content of DNA in tail (by the relative tail fluorescence intensity in percent to the untreated control), L/H (relation of a comet length to its height) and OTM (the product of a tail length and the ...

Evaluation of the in vitro cytogenotoxicity profile of antipsychotic drug haloperidol using human peripheral blood lymphocytes
G Gajski, M Geric, V Garaj-Vrhovac - Environmental Toxicology and Pharmacology, 2014
... with EtBr (20 µg/ml) and analyzed at 200× magnification using an epifluorescence microscope Olympus BX51 (Tokyo, Japan) connected through a black and white CCD camera to an automatic digital analysis system (Comet Assay IV; Perceptive Instruments Ltd., Haverhill ...

Evaluation of the mutagenicity and genotoxic potential of carvacrol and thymol using the Ames Salmonella test and alkaline, Endo III-and FPG-modified comet …
M LLana-Ruiz-Cabello, S Maisanaba, M Puerto… - Food and Chemical …, 2014
... DP manager). Images of randomly selected nuclei (>100) per experimental point were analysed with image analysis software (Comet Assay IV, Perceptive Instruments, UK). 2.5.2. Enzyme-modified Comet assay. To investigate ... .

Ocean acidification increases copper toxicity to the early life-history stages of the polychaete Arenicola marina in artificial seawater.
AL Campbell, S Mangan, RP Ellis, C Lewis - Environmental Science & Technology, 2014
... One hundred cells per slide were analysed using Comet Assay IV (Perceptive Instruments Ltd) to quantify the percentage of DNA in the comet tail (resulting from DNA strand breaks) to approximate the percentage of DNA damage induced in each treatment. ... .

The protective effects of vitamin C on the DNA damage, antioxidant defenses and aorta histopathology in chronic hyperhomocysteinemia induced rats
M Boyacioglu, S Sekkin, C Kum, D Korkmaz, F Kiral… - … and Toxicologic Pathology, 2014
... Measurements of tail intensity and tail moment of 'comets' were made by a computer-based image analysis system (Comet Assay IV, Perceptive Instruments, UK) for 100 randomly selected cells, ie 50 cells from each of two gels from each sample. ...

Genotoxic and mutagenic potential of nitramines
LM Fjellsbø, S Verstraelen, A Kazimirova… - Environmental Research, 2014
... solution to spread and cover all gels. The slides were analysed by a fluorescent microscope (Leica DMI 6000 B) using an image analysis system Comet assay IV (Perceptive Instruments Ltd.). The software is linked to a closed ...

Extracellular signal-regulated kinases 1/2 and Akt contribute to triclosan-stimulated proliferation of JB6 Cl 41-5a cells
Y Wu, FA Beland, S Chen, JL Fang - Archives of Toxicology, 2014
... using a Nikon eclipse 50i fluorescence microscope (Nikon Instru- ments Inc., lewisville, Tx), and the images were captured with image analysis software (comet Assay IV, version 4.2, Perceptive Instruments, Suffolk, uK). At least ...

Induction and repair of DNA damage measured by the comet assay in human T lymphocytes separated by immunomagnetic cell sorting
J Bausinger, G Speit - … Research/Fundamental and Molecular Mechanisms of …, 2014
... slide. Measurements were made by image analysis (Comet Assay IV, Perceptive Instruments, Haverhill, UK) and DNA migration was determined by measuring the “tail intensity” (% tail DNA). 2.5. Statistical analysis. Pre-experiments ...

Can the use of medical muds cause genotoxicity in eukaryotic cells? A trial using comet assay
G Gerencsér, K Szendi, K Berényi, C Varga - Environmental Geochemistry and Health
... 1988 ; Hellman et al. 1999 ; Jatte and Klara 2003 ). Fifty nuclei were analyzed on each slide by using Comet assay IV. Software (Perceptive Instruments Ltd., 400×). The ... (Perceptive Instruments Ltd., 400×). Statistical analysis. The ...

Positional Isomerization Of A Non-Cleavable Combi-Molecule Dramatically Altered Tumour Cell Response Profile
Y Huang, Z Rachid, L Peyrard, ZS Mouhri, C Williams… - Chemical Biology & Drug …, 2014
... DNA damage was quantified using Comet Assay IV software (Perceptive Instruments, UK) and the degree of DNA damage was expressed as a tail moment parameter (ie, the distance between the bary center of the head and ...

Citral and eugenol modulate DNA damage and pro-inflammatory mediator genes in murine peritoneal macrophages
M de Paula Porto, GN da Silva, BCO Luperini… - Molecular Biology Reports
... After staining with SYBR Gold (1:10,000—Invitrogen), 50 randomly selected “nucleoids” per slide were examined at 400X magnification with a fluorescence microscope using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Effects of defined mixtures of persistent organic pollutants on DNA damage in a human endocrine cell line (H295R) and in mouse lymphocytes exposed in vitro and in …
I Haukeli - 2014
in a Human Endocrine Cell Line (H295R) and in Mouse Lymphocytes Exposed in Vitro and in Vivo Norwegian University of Life Sciences ... ...image analysis program;comet assay IV 4,2 Perceptive Instruments Ltd. 50 comets per gel ...

Single Cell Gel Electrophoresis (SCGE) and Pig-a mutation assayin vivo-tools for genotoxicity testing from a regulatory perspective: a study of benzo [a] …
IA Graupner, C Instanes, SD Dertinger, JM Andersen… - Mutation Research/Genetic …, 2014
... cells per replicate were scored using 20 × magnification in an Olympus BX51 microscope (light source: Olympus BH2-RFL-T3, Olympus optical Co., Ltd.; camera: A312f-VIS, BASLER, Ahrensburg, Germany) with the Comet IV analysis software (Perceptive Instruments Ltd., Bury ...

Nicotine, cotinine, and ß-nicotyrine inhibit NNK-induced DNA-strand break in the hepatic cell line HepaRG
Ordonez P, Sierra AB, Camacho OM, Baxter A, Banerjee A, Waters D, Minet E Toxicol In Vitro. 2014 Jul 15.
… DNA damage was determined using COMET Assay IV image analysis software (Perceptive Instruments, Harverhill, UK) and the percentage of tail intensity was recorded. At least three independent assays were performed...

Aroclor 1254-induced genotoxicity in male gonads through oxidatively damaged DNA and inhibition of DNA repair gene expression
SM Attia, SF Ahmad, RM Okash, SA Bakheet - Mutagenesis, 2014
... Two slides were prepared for each animal; images from 100 cells (50 from each replicate slide) were randomly selected and subjected to image analysis using software Comet Assay IV (Perceptive Instruments, Suffolk, UK). ...

Identification of a Putative Tdp1 Inhibitor (CD00509) by in Vitro and Cell-Based Assays
RA Dean, HK Fam, J An, K Choi, Y Shimizu, SJM Jones… - Journal of Biomolecular …, 2014
... Images were acquired using a Zeiss Axiovert 200 microscope, a Zeiss AxiocamMR camera, and the Zeiss Axiovision imaging system. Comet tail analysis was done using the Comet Assay IV software by Perceptive Instruments (Haverhill, UK). MTT Assay. ...

Targeting BRCA1-BER deficient breast cancer by ATM or DNA-PKcs blockade either alone or in combination with cisplatin for personalized therapy
N Albarakati, T Abdel-Fatah, R Doherty, R Russell… - Molecular Oncology, 2014
... Slides were left to dry overnight at 4 °C and then each sample stained with 50 µl Sybr green and scored using COMET ASSAY IV software (Perceptive Instruments Ltd, Bury St Edmonds, UK). Treatments were performed in triplicate and 50 cells counted for each.

Mechanism-based genotoxicity screening of metal oxide nanoparticles using the ToxTracker panel of reporter cell lines.
Karlsson HL, Gliga AR, Calléja F, Gonçalves C, Wallinder I, Vrieling H, Fadeel B, Hendriks G. Part Fibre Toxicol. 2014
The comets were examined on a fluorescence microscope (Leica DMLB and Leica Axioplan2, Houston, TX) with Comet Assay IV (Perceptive Instruments) and ...

Sex-specific dose-response analysis of genotoxicity in cyproterone acetate-treated F344 rats
W Ding, ME Bishop, MG Pearce, KJ Davis, GA White… - Mutation Research/Genetic …, 2014
... Two slides were scored from each tissue; 75 cells were selected randomly from each slide and scored using a system consisting of a Nikon 50i fluorescent microscope and Comet IV digital imaging software (Perceptive Instruments, Wiltshire, UK). 2.4. ...

Differential effects of triclosan on the activation of mouse and human peroxisome proliferator-activated receptor alpha
Y Wu, Q Wu, FA Beland, P Ge, MG Manjanatha… - Toxicology Letters, 2014
... labeled DNA was visualized (magnification, 200×) using a Nikon Eclipse 50i fluorescence microscope (Nikon Instruments Inc., Lewisville, TX) and the images were captured with image analysis software (Comet Assay IV, version 4.2, Perceptive Instruments, Suffolk, UK). ...

Penicillium sp. as an organism that degrades endosulfan and reduces its genotoxic effects
M Romero-Aguilar, E Tovar-Sánchez… - SpringerPlus, 2014
... All of the steps were conducted in dim light to prevent additional nonspecific DNA breakage. The comet images were captured, and the Comet IV software (Perceptive Instruments) was employed to measure various comet parameters. ...

Genotoxic effects of CdS quantum dots and Ag 2 S nanoparticles in fish cell lines (RTG-2)
M Munari, J Sturve, G Frenzilli, MB Sanders, A Brunelli… - Mutation Research/Genetic …, 2014
... Air-dried slides were stained with ethidium bromide (2 µg/ml in dH 2 O) and scored using Comet Assay IV (Perceptive Instruments Ltd). % Tail DNA was chosen as a reliable comet assay parameter [27]. 50 cells for each half slide were scored. ...

Innate Inflammation Induced by the 8-Oxoguanine DNA Glycosylase-1–KRAS–NF-?B Pathway
L Aguilera-Aguirre, A Bacsi, Z Radak, TK Hazra, S Mitra… - The Journal of Immunology, 2014
... The agarose was air-dried, and the DNA was stained with SYBRGreen. Comets were analyzed using the Comet Assay IV version 4.2 system image analysis software (Perceptive Instruments, Suffolk, UK) (12). Depending on cell availability, 120–160 comets were scored. ...

Expression of a pathogenic mutation of SOD1 sensitises aprataxin deficient cells and mice to oxidative stress and triggers hallmarks of premature ageing
J Caroll, TKW Page, SC Chiang, B Kalmar, D Bode… - Human Molecular Genetics, 2014
... fluorescence microscopy (Nikon Eclipse E400) at 20 X magnification. Comet tail moment for 100 cells per sample was determined using Comet Assay IV software (Perceptive Instruments). Telomere stability assays Telomere ...

5 FU resistance abrogates the amplified cytotoxic effects induced by inhibiting checkpoint kinase 1 in p53 mutated colon cancer cells
T Akasaka, M Tsujii, J Kondo, Y Hayashi, J Ying, Y Lu… - International Journal of Oncology
... After staining with 20 µg/ml ethidium bromide for 1 min, we quantified the DNA damage of 100 randomly selected cells using the Comet Assay IV software (Perceptive Instruments Ltd., Suffolk, UK) on a computer attached to a fluorescence microscope (Olympus, Tokyo, Japan). ...

High throughput sample processing and automated scoring
G Brunborg, P Jackson, S Shaposhnikov, H Dahl… - Name: Frontiers in Genetics, 2014
... et al. 2010). Scoring is done either with a semi-automated system (Comet Assay IV, Perceptive Instruments, Bury St. Edmunds, Suffolk, UK), or with the  ...

The determination of possible genetic damage to women undergoing in vitro fertilization due to infertility caused by the male factor
S Özden, D Yüzbasioglu, F Ünal, M Özel - Food and Chemical Toxicology, 2014
... The tail intensity (%) and tail length (%) of 100 comets on each slide (a total of 200 comets per donor) were determined, using the specialized Image Analysis System (“Comet Assay IV,” Perceptive Instruments Ltd., UK). 2.5. Statistical analysis. ...

Hepatic toxicology following single and multiple exposure of engineered nanomaterials utilising a novel primary human 3D liver microtissue model
A Kermanizadeh, M Løhr, M Roursgaard, S Messner… - Particle and Fibre …, 2014
…The percentage of DNA in the tail was measured using an automatic image analyser (Comet Assay IV; Perceptive Instruments, UK) connected to a…

Protective Effects of a New Phloretin Derivative against UVB-Induced Damage in Skin Cell Model and Human Volunteers
S Shin, H Kum, D Ryu, M Kim, E Jung, D Park - International Journal of Molecular …, 2014
…The percentage of DNA in the tail was measured using an automatic image analyser (Comet Assay IV; Perceptive Instruments, UK) connected to a…

Recent advances in in vivo genotoxicity testing: prediction of carcinogenic potential using comet and micronucleus assay in animal models.
Kang SH, Kwon JY, Lee JK, Seo YR. J Cancer Prev. 2013 Dec;18(4):277-88.
...quantitated via image analyzer system such as Comet IV software (Perspectives, UK). For each sample, one hundred comet cells are subjected...

Protective and restorative potency of Vitamin D on persistent biochemical autistic features induced in propionic acid-intoxicated rat pups.
Alfawaz HA, Bhat RS, Al-Ayadhi L, El-Ansary AK. 2014 Oct 25;14(1):416.
...a camera to a computer-based image analysis system (Comet Assay IV software, Perspective Instruments). ..

Single Nucleotide Polymorphisms in Noncoding Regions of Rad51C Do Not Change the Risk of Unselected Breast Cancer but They Modulate the Level of Oxidative …
P Gresner, J Gromadzinska, E Jablonska, M Stepnik… - PLOS ONE, 2014
... Finally, the slides were stained with 5 µg/ml DAPI and 50 cells from each slide were analyzed using an Olympus fluorescence microscope (a BX40 instrument; Olympus, Tokyo, Japan) equipped with an image analysis system (Comet IV, Perceptive Instruments, UK). ...

In vivo genotoxicity of estragole in male F344 rats
W Ding, DD Levy, ME Bishop, MG Pearce, KJ Davis… - … and Molecular Mutagenesis, 2014
.... slides were scored from each tissue/treatment/sampling time; at least 100 cells were selected randomly from each slide and scored using a system consisting of a Nikon 501 fluorescent microscope and Comet IV digital imaging software (Perceptive Instruments, Wiltshire, UK). ...

Genotoxicological Studies of Lower Stretch of the Sava River
B Vukovic-Gacic, S Kolarevic, K Sunjog, J Tomovic… - The Sava River, 2014
... head to tail length ratio [25]. In recent studies, the comets are scored and analyzed using the Comet IV Computer 438 B. Vukovic-Gacic et al. Page 449. Software (Perceptive Instruments, UK)(Fig. 1). Tail intensity, TI (the percent ...

Toxicity assessment of aggregated/agglomerated cerium oxide nanoparticles in an in vitro 3D airway model: the influence of mucociliary clearance
CF Kuper, M Gröllers-Mulderij, T Maarschalkerweerd… - Toxicology in Vitro, 2014
... For scoring, each slide was stained with ethidium bromide. 50 randomly chosen cells were analysed per slide (Comet Assay IV, Perceptive Instruments Ltd, Suffolk, UK). In total 300 cells per treatment concentration were scored. ...

Perfluoroalkylated substances (PFAS) affect oxidative stress biomarkers in vitro
M Wielsøe, M Long, M Ghisari, EC Bonefeld-Jørgensen - Chemosphere, 2014
... The gels were stained with SYBR Gold from Invitrogen (Denmark), and 100 cells/gel were scored and analyzed using Comet Assay IV software from Perceptive Instruments. 2.4. Detection of reactive oxygen species (ROS). The ...

Cytotoxicity and genotoxicity of panel of single-and multi-walled carbon nanotubes: in vitro effects on normal Syrian Hamster Embryo (SHE) and immortalized V79 …
C Darne, F Terzetti, C Coulais, C Fontana, S Binet…
... Images of 100 randomly selected comets were acquired and analyzed for each sample (Comet assay IV, Perceptive Instruments, UK.) in order to evaluate the % tail DNA used as a measure of DNA damage. The presence of CNTs did ...

The cosmetic dye quinoline yellow causes DNA damage in vitro
FMD Chequer, V de Paula Venâncio… - Mutation Research/Genetic …, 2014
... and the displacement of the tail's center of mass relative to the center of the head) and the tail intensity (ie, the percentage of DNA in the tail) values for 100 nucleoids were evaluated at 400× magnification using the Comet Assay IV software (Perceptive Instruments, Suffolk, UK). ...

Molybdenum nanoparticles-induced cytotoxicity, oxidative stress, G2/M arrest, and DNA damage in mouse skin fibroblast cells (L929)
MA Siddiqui, Q Saquib, M Ahamed, NN Farshori… - Colloids and Surfaces B: …, 2014
... camera. Images from 100 cells (50 from each replicate slide) were randomly selected and subjected to image analysis using software Comet Assay IV (Perceptive Instruments, Suffolk, UK). 2.14. Statistical analysis. Results were ...

Novel method for the high-throughput processing of slides for the comet assay
M Karbaschi, MS Cooke - Scientific Reports, 2014
... All slides were then observed and scored by fluorescent microscopy (50 cells per gel; 100 cells per treatment), and percentage tail DNA of the comets was recorded, using comet assay IV analysis software, version 4.2 (Perceptive Instruments, Haverhill, Suffolk, UK). ...

Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure, part 2: Intratracheal instillation and intravenous injection
Y Guichard, MA Maire, S Sébillaud, C Fontana… - … and Molecular Mutagenesis, 2014
... For each tissue, 100 cells per animal were analyzed using the Comet Assay IV software (Perceptive Instruments, Suffolk, UK), and the percentage of DNA in the tail of each comet was measured. The median was determined from these 100 comet results. Micronucleus Assay. ...

Resveratrol Modulates the Topoisomerase Inhibitory Potential of Doxorubicin in Human Colon Carcinoma Cells
A Schroeter, D Marko - Molecules, 2014
... After staining of the cells with 10 µg/mL ethidium bromide solution the tail intensity (amount of nuclear DNA found in the tail) was determined by the software CometIV (Perceptive Instruments, Suffolk, UK). 3.5. Intracellular Concentration of DOX ...

Influence of ozone initiated processing on the toxicity of aerosol particles from small scale wood combustion
EZ Nordin, O Uski, R Nyström, P Jalava, AC Eriksson… - Atmospheric Environment, 2014
... The nuclei were analyzed in a blinded manner (100 nuclei per dose) using an image analysis system (Comet assay IV, Perceptive Instruments Ltd., UK). The Olive tail moment ([(tail mean-head mean) × tail % DNA/100]) was the parameter used in the statistical analysis. ...

Investigation on the toxic potential of Tribulus terrestris in vitro
M Abudayyak, AT Jannuzzi, G Özhan, B Alpertunga - Pharmaceutical Biology, 2014
... BX53, Olympus, Tokyo, Japan) at 400× magnification. Results were visualized using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). One hundred cells were scored per concentration ...

Can the comet assay be used reliably to detect nanoparticle-induced genotoxicity?
HL Karlsson, S Di Bucchianico, AR Collins, M Dusinska - … and Molecular Mutagenesis, 2014
... the slides are preferably fixed and DNA can then be stained with a DNA-specific dye, (eg, ethidium bromide or SYBR Green), after which the comets are analyzed by fluorescence microscopy using an image analysis system such as Comet assay IV (Perceptive Instruments). ...

Molybdenum nanoparticles-induced cytotoxicity, oxidative stress, G2/M arrest, and DNA damage in mouse skin fibroblast cells (L929)
MA Siddiqui, Q Saquib, M Ahamed, NN Farshori… - Colloids and Surfaces B: …, 2014 - Elsevier
... slide) were randomly selected and subjected to image analysis using software Comet Assay IV ... 29%, and 60% at 25, 50, and 100 µg/ml of Mo-NPs, respectively ... A Molybdenum nanoparticlesinduced reduction in the intensity of mitochondrial membrane potential (MMP) in L929 ...

Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route
A Tarantini, S Huet, G Jarry, R Lanceleur, M Poul… - … and Molecular Mutagenesis, 2014
... Before scoring, slides were stained with 20 µg/ml propidium iodide. Analyses were performed with a fluorescence microscope using the Comet IV software (Perceptive instruments) and 50 nucleoids were scored on each of the two slides per test condition. ...

Clinical and cellular roles for TDP1 and TOP1 in modulating colorectal cancer response to irinotecan
C Meisenberg, DC Gilbert, A Chalmers, V Haley… - Molecular Cancer …, 2014
... Green (1:10,000 in PBS) for 10 min. The tail moments for 100 cells per sample were scored using the Comet Assay IV software (Perceptive Instruments, UK). Immunostaining Cell monolayers grown to sub-confluent densities on coverslips were treated with media ...

Lipoic acid induces p53-independent cell death in colorectal cancer cells and potentiates the cytotoxicity of 5-fluorouracil
B Dörsam, A Göder, N Seiwert, B Kaina, J Fahrer - Archives of Toxicology, 2014
... Obtained comets were analyzed by microscopy using an Olympus BX50 equipped with a ColorView camera (Olympus, Münster, Germany). In each experiment, 50 cells were scored using Comet IV software (Perceptive Instruments Ltd., Bury St Edmunds, UK). ...

Quotes

Total change in our lab since you came (with Comet Assay IV of course...)! thanks... :-)

Postgraduate Researcher, University, France

I love the software. Keep up the good work over there.

Study Director, Contract Research Organisation, USA

I'm really a fan of your system :)

Genetic Toxicologist, Global Pharmaceutical Company

We are using the Comet Assay IV a lot (and our use is increasing). It's working very well.

Professor, Children's Research Hospital, USA

So it's working out fine and we're very happy!

Toxicologist, Health Agency, Canada

We have just recently utilized the system and are extremely pleased.

Assistant Professor, University, USA

Everything has gone very well with the system and we are very pleased with it.

Professor, University, UK

We have had no problems so far, and we are very pleased with the system.

Senior Researcher, Public Health Agency, Finland

Excellent, the training was very helpful and specific to in-house technicalities.

Lecturer, University, UK

The installation was quick and all the details were described and easily understandable for the technicians.

Head of Department, Global Chemical Company, Germany

The system was set up last month, and so far it works very well. Both my boss and I are very satisfied with it and like it very much.

Researcher, University Hospital, Germany

We started using Comet Assay IV and it works well...

Researcher, Nuclear Agency, France

It's an amazing program! Really useful!

Research Scientist, University, Brazil

We are impressed indeed! It has saved us quite a lot of time and pains.

Director, Toxicology Research Centre, Spain

We have installed the software and are using it without any problem. It's really simple! My PhD students are very happy with it.

Professor, University, Spain

Comet Assay IV software is in use. Currently our comet analysis is based on tail moment measurement and I must say that everything works properly.

Researcher, Cancer Research Institute, Slovakia

...Everything is fine with the Comet Assay IV system...and I'm pretty happy about this new software with just clicking on the cells instead of drawing a frame around them

Researcher, Cancer Research Organisation, UK

So far, I'm very happy with the system.

Research Lecturer, University, UK

The system is up and running. Everybody is impressed with the speed of scoring slides.

Researcher, University, South Africa

The Comet Assay IV system we already installed and is working perfectly.

Scientist, Environmental Institute, Norway

The current Comet Assay IV system works without any trouble…we are pleased with the system.

Scientist, Chemical Company, Japan

Yes, it is very useful and we are in the process of writing a paper also from the data derived from Comet Assay IV.

Assistant Professor, University, USA

It is actually working great.

Professor, University, USA

Excellent, perfectly straightforward.

Scientist, University, UK

We are really very happy with the comet analysis system.

Research Scientist, University, Austria

...and the system is running great.

Medical Researcher, Cancer Research Institute, USA

It is, indeed, much quicker than the old image analysis system that we had. We are very pleased with it.

Senior Researcher, Public Health Agency, Finland

Thanks for your attention and great service. I am so glad that we have Comet IV.

Study Director, Contract Research Organisation, USA

Perfect and very clear installation and training!

Researcher, Contract Testing Laboratory, Europe

We're now using the software and it works great. It has cut our time for comets by at least half.

Post Doctoral Fellow, National Institute of Health, USA

I have been using the Comet Assay IV Software. It is great! During my software validation, I realized how much easier and precise it is compared to our old software. It makes analyzing the comet slides more enjoyable

Scientist, Medical Research Centre, USA

We have found the Comet Assay IV software very useful and convenient.

Research Scientist, Food Safety Agency, France

We are very satisfied with your software.

Scientist, Cancer Research Institute, Slovakia

Everything has gone very well with the system and we are very pleased with it.

Scientist, University, UK

All is going well and the more I use the software the more I love the way it's so simple and intuitive...the comet software is running well and in increasing use.

Scientist, University, UK

I don't have any questions about the software as it has worked quite well for us...so far we are very happy with the software.

Research Scientist, University, Australia

...we are "happy users" of the software

Senior Researcher, Risk Assessment Institute, Germany

The system is working great.

Senior Research Scientist, Cancer Research Institute, UK

We are very happy with everything, it is easy to work with your software.

Senior Scientist, National Health Institute, Portugal

...have been using it now for a couple of weeks and it seems to be working great. Very user friendly - I love it as does my intern!

Staff Chemist, Marine Laboratory, USA

Just to let you know we have started the validation study for Comet Assay IV and it has been going very well! We ran a comet assay last week and scored the slides with no problems at all. I am very pleased with the system and it will certainly make a difference when we run some comet studies this year!

Study Director, Food & Household Products Company, UK

About Us

This site is maintained by Perceptive Instruments, industry-leading experts in image analysis for the comet assay.

We regularly attend comet assay workshops and contribute to the debate towards regulatory acceptance of the assay.

About Comet Assay IV

Comet Assay IV is the world's fastest, most consistent comet assay scoring system.

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