Papers from 2015

Recommendations for increasing alkaline comet assay reliability in plants
B Pourrut, E Pinelli, VC Mendiola, J Silvestre, F Douay - Mutagenesis, 2015
... For each slide, 50 randomly selected nuclei were analysed using an Olympus fluorescence microscope with an excitation filter of 510–560nm and a barrier of 590nm. A computerized image analysis system (Comet Assay IV; Perceptive Instruments) was employed. ...

DNA repair after X-irradiation: lessons from plants
J Einset, AR Collins - Mutagenesis, 2015
... Analysis of comets was performed after staining with 1 µg/ml 4'-6-diamidino-2-phenylindole using a Nikon Eclipse TS100 fluorescence microscope equipped with an Allied Vision Technologies camera and the Comet Assay IV image analysis program (Perceptive Instruments). ...

Effect of silver nanoparticles on mitogen-activated protein kinases activation: role of reactive oxygen species and implication in DNA damage
A Rinna, Z Magdolenova, A Hudecova, M Kruszewski… - Mutagenesis, 2015
... O (10min) at 4°C. Samples were stained with 20 µl SYBR gold (0.1 µl/ml in Tris-EDTA buffer: 10mM Tris–HCl, 1mM EDTA, pH 7.5–8) and 100 comets (50 per gel) for each sample were scored by computerised image analysis (Comet Assay IV 4.2, Perceptive Instruments Ltd) for ...

Environmental concentrations of benz-a-anthracene induce developmental defects and DNA damage and impair photomotor response in Japanese medaka …
F Le Bihanic, V Sommard, A Pichon, J Grasset… - … and Environmental Safety, 2015
.... Larvae were rinsed, anesthetized and then photographed using an epifluorescence microscope at X100 magnification (U-MWIG2 mirror unit, Olympus, Rungis, France) equipped with a monochrome CCD camera (Perceptive instruments, Bury St Edmunds, UK). ...

Cytotoxic and genotoxic responses of the RTgill-W1 fish cells in combination with the yeast oestrogen screen to determine the sediment quality of Lagos lagoon, …
NH Amaeze, S Schnell, O Sozeri, AA Otitoloju… - Mutagenesis, 2015
... 5min. Comets where analysed using a Leica fluorescence microscope (Leica DMLB 020-519-010 LB30T). DNA damage was scored using the Comet IV capture system (version 4.11; Perceptive Instruments, UK). Fifty nucleoids ...

Assessment of DNA damage using comet assay in middle-aged overweight/obese subjects after following a hypocaloric diet supplemented with cocoa extract
I Ibero-Baraibar, A Azqueta, AL de Cerain, JA Martinez… - Mutagenesis, 2015
... on one slide. Comets were visualised under a fluorescence microscope (NIKON Eclipse 50 i) and the semi-automated image analysis system Comet Assay IV (Perceptive Instruments) was used to evaluate them. Fifty comets ...

Does the duration of lysis affect the sensitivity of the in vitro alkaline comet assay?
JM Enciso, O Sánchez, AL de Cerain, A Azqueta - Mutagenesis, 2015
... were visualised under a fluorescence microscope (NIKON Eclipse 50 i). DNA damage was quantified in 100 randomly selected comets per slide (50 comets in each gel) by measuring the % tail DNA using the image analysis software Comet Assay IV (Perceptive Instruments Ltd ...

Rejoining kinetics of bleomycin-induced DNA single-strand breaks in agarose-bound human blood cells
Y Lu, R Matsuse, T Sobue - Mutation Research/Genetic Toxicology and …, 2015
... After staining with 10 μg/mL ethidium bromide for 1 min, we quantified the DNA damage of 40∼60 leukocytes from each well under a microscope at 100×magnification using a Comet Assay IV imaging system (Perceptive Instruments, Haverhill, UK) attached to a fluorescent ...

How can age and lifestyle variables affect DNA damage, repair capacity and endogenous biomarkers of oxidative stress?
JP Soares, AM Silva, S Fonseca, MM Oliveira… - Experimental Gerontology, 2015
... Slides were stained with ethidium bromide (20 µg/mL) immediately before visualization of comet DNA using a Nikon Eclipse E400. DNA damage estimation was evaluated by the image analysis software Comet IV (Perceptive Instruments, Ltd.) (Fig. ...

Genotoxic and biochemical changes in Baccharis trimera induced by coal contamination
APS Menezes, J Da Silva, RR Rossato, MS Santos… - … and Environmental Safety, 2015
... and visualization of slides was performed through a fluorescence microscope (Olympus BX 41, excitation filter, 515–560 nm; barrier filter, 590 nm) with a 200× magnification, (10) the analysis was performed using the Comet Assay IV software (Perceptive instruments, UK) and...

Evaluation of the repeated-dose liver, bone marrow and peripheral blood micronucleus and comet assays using kojic acid
Y Ogiwara, M Sugiura, K Watanabe, J Tawara, E Endo… - Mutation Research/Genetic …, 2015
... Comets were measured via a digital camera linked to an image analyzer (Comet IV, Perceptive Instruments, Suffolk, UK) using a fluorescent microscope (BX53-34FL-1, Olympus Co., Ltd., Tokyo, Japan) with a blue excitation filter at 200× magnification. ...

Oxidative DNA damage in diabetic and mild gestational hyperglycemic pregnant women
RB Gelaleti, DC Damasceno, PH Lima, DM Salvadori… - Diabetology and Metabolic Syndrome …, 2015
... The dried slides were stained with ethidium bromide (20 µg/ml in distilled H2O; 50 µl/slide), covered with a coverslip and analysed in a fluorescence microscope connected to a computer- based analysis system (Comet Assay IV, Perceptive Instruments, UK) to determine the ...

Cytotoxicity and genotoxicity assessment of silver nanoparticles in mouse
MY Jones, WA Monroe, PC Howard, T Zhou, T Chen... Nanotoxicology, 2015
... Two slides were scored from each sample; at least 150 cells were selected randomly for each sample and scored using a system consisting of a Nikon 501 fluorescent microscope and Comet IV digital imaging software (Perceptive Instruments, Wiltshire, UK). ...

Toxicity of the micropollutants Bisphenol A, Ciprofloxacin, Metoprolol and Sulfamethoxazole in water samples before and after the oxidative treatment
J Richard, A Boergers, C vom Eyser, K Bester, J Tuerk - International Journal of Hygene and Environmental Health, 2014
... to dry. The analysis was performed using a CCD camera attached to a fluorescence microscope (Leica DMBL, Leitz GmbH and Co KG, Wetzlar, D) and the Comet Assay 4 Software (Perceptive Instruments, Suffolk, UK). The ...

Rescue of cells from apoptosis increases DNA repair in UVB exposed cells: implications for the DNA damage response
M Karbaschi, S Macip, MD Evans, V Mistry, HHK Abbas… - Toxicology Research, 2015
... artefactual DNA damage. Comets were visualised by using a combination of an on-line CCD camera, fluorescence microscopy at ×20 magnification, and Comet Assay IV software version 4.2 (Perceptive Instruments, Suffolk, UK). 50 cells per gel (100 cells in duplicate ...

Protective effect of C. sativa leaf extract against UV mediated-DNA damage in a human keratinocyte cell line
IF Almeida, AS Pinto, C Monteiro, H Monteiro, L Belo… - Journal of Photochemistry …, 2015
... dry. Comets were visualized by fluorescence microscopy at ×200 magnification. Images were captured by an on-line CCD camera and analyzed with the Comet IV software version 4.3 (Perceptive Instruments, Suffolk, UK). A ...

Eco-genotoxicity of six anticancer drugs using comet assay in daphnids.
A Parrella, M Lavorgna, E Criscuolo, C Russo… - Journal of Hazardous Materials …, 2015 - Elsevier
... each of four/six concentrations) were scored using Comet assay IV image analysis software (Perceptive Instrument, UK ... Just recently, IM eco-genotoxicity was assessed in higher plants ... cause a direct damage on DNA even if a genetic instability was detected by the Comet assay. ...

Reference cells and ploidy in the comet assay
G Brunborg, AR Collins, A Graupner, KB Gutzkow… Frontiers in Genetics, 2015
... scoring of FE and PBMN comets, dried GelBond films® were rehydrated and stained with 115 SYBRGold (Life Technologies Ltd, Paisley, UK; 2 µL in 25 mL TE buffer pH7.4, 20 minutes at RT), 116 rinsed in water, and analyzed with Comet IV (Perceptive Instruments Ltd, Bury St. ...

Adaptation of HepG2 cells to silver nanoparticles-induced stress is based on the pro-proliferative and anti-apoptotic changes in gene expression
K Brzóska, S Meczynska-Wielgosz, TM Stepkowski… - Mutagenesis, 2015
... X-irradiation was carried out in an ice water bath, with the use of a Smart200 (Yxlon) defectoscope operating at 200kV and 4.5 mA, with 3mm Al filtration. Image analysis of the data was performed by the Comet Assay IV Image Analysis System (Perceptive Instruments, UK). ...

Genotoxicity of tungsten carbide–cobalt (WC–Co) nanoparticles in vitro: Mechanisms-of-action studies
H Moche, D Chevalier, H Vezin, N Claude, E Lorge… - Mutation Research/Genetic Toxicology …, 2015
... and Scientific Instruments Group, Heerbrugg, Switzerland) equipped with an excitation filter of 515–560 nm and a 590 nm barrier filter, connected through a gated CCD camera to Comet Image Analysis System software, version 4.0 (Perceptive Instruments Ltd., Haverhill, United ...

Increased levels of chromosomal aberrations and DNA damage in a group of workers exposed to formaldehyde
S Costa, S Carvalho, C Costa, P Coelho, S Silva… - Mutagenesis, 2015
... Microscopic analyses were performed on a Nikon Eclipse E400 Epi-fluorescence microscope (G2A filter, Nikon C-SH61). Image capture and analysis were performed with Comet Assay IV software (Perceptive Instruments). The ...

Use of a standardized JaCVAM in vivo rat comet assay protocol to assess the genotoxicity of three coded test compounds; ampicillin trihydrate, 1,2-dimethylhydrazine dihydrochloride, and N-nitrosodimethylamine
JP McNamee, PV Bellier - Mutation Research/Genetic Toxicology and …, 2015
... Technologies Inc.). The slides were then analyzed by fluorescence microscopy (Olympus BX60 microscope, NB filter cube) at 200× magnification using the Comet Assay IV image analysis system (Perceptive Instruments, UK). For ...

Comet assay evaluation of six chemicals of known genotoxic potential in rats
CA Hobbs, L Recio, M Streicker, MH Boyle, J Tanaka… - Mutation Research/Genetic …, 2015
... After staining slides with SYBR ® Gold (Molecular Probes, Invitrogen, Carlsbad, CA, USA), 100 cells were scored per sample using comet IV image analysis software (Perceptive Instruments, Ltd., Suffolk, UK). Slides were coded and scored without knowledge of their identity. ...

Genotoxic properties of representatives of alkylindazoles and aminoalkyl-indoles which are consumed as synthetic cannabinoids
VJ Koller, F Ferk, H Al-Serori, M Mišík, A Nersesyan… - Food and Chemical …, 2015
... Finally, air-dried slides were stained with propidium iodide (10 µg/mL) and the percentage of DNA in the tails measured by use of a computer aided image analysis system (Comet IV, Perceptive Instruments Ltd., Haverhill, UK). ...

Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1 fish liver cell line
A Kienzler, BJ Mahler, PC Van Metre, N Schweigert… - Science of The Total Environment…, 2015
... Ltd., Oberkochen, Germany). For each slide, %TI was measured in 50 randomly selected cells using image analysis software (Comet IV, Perceptive Instruments, Ltd., Haverhill, UK), and the median computed. A greater degree ...

Aryl hydrocarbon receptor ligand 5F 203 induces oxidative stress triggering DNA damage in human breast cancer cells
L McLean, C Watkins, P Campbell, D Zylstra… - Chemical Research in Toxicology…, 2015
... Key words: aryl hydrocarbon receptor (AhR), DNA damage, reactive oxygen species (ROS), ... with low estrogen receptor alpha relative to estrogen receptor beta protein expression levels ... a fluorescence microscope and the Comet Assay IV software scoring system (Perceptive ...

Combination comet/micronucleus assay validation performed by BioReliance under the JaCVAM initiative
K Pant, L Krsmanovic, SW Bruce, T Kelley, M Arevalo… - Mutation Research/Genetic Toxicology …, 2015
... Fifty randomly selected, non-overlapping cells (comets) per slide were scored at 200× magnification for DNA damage using the fully validated automated scoring system Comet Assay IV, v. 4.11 from Perceptive Instruments Ltd. (UK). ...

Integrated Use Of Different Fish Related Parameters To Assess The Status Of Water Bodies
M Lenhardt, V Poleksic, B Vukovic-Gacic, B Raškovic… - Slovenian Veterinary Research …, 2015
... degrees and types of pollution. Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). Gills gave the best response as compared to other tissues. Gills may be more prone ...

Impact of anatase and rutile titanium dioxide nanoparticles on uptake carriers and efflux pumps in Caco-2 gut epithelial cells
M Dorier, B Emilie, G Veronesi, F Barreau… - Nanoscale, 2015
... min. Slides were neutralized with 0.4 M Tris pH 7.5 and stained with ethidium bromide before observation. Comet analysis was achieved with Comet IV software (Perceptive instruments, Suffolk, UK). C Oxidative stress assays. ... (UK). ...

Effects of maternal vitamin B6 deficiency and over-supplementation on DNA damage and oxidative stress in rat dams and their offspring
MR Almeida, VP Venâncio, AF Aissa, JDC Darin… - Food and Chemical Toxicology…, 2015
... One hundred nucleoids per animal were randomly analyzed by the software Comet Assay IV TM version 4.3 (Perceptive Instruments, Bury St. Edmunds, Suffolk, UK), and the “Tail intensity (%DNA in the tail)” parameter was used to measure the DNA damage. ...

An improved method for the isolation of rat alveolar type II lung cells: Use in the Comet assay to determine DNA damage induced by cigarette smoke
A Dalrymple, P Ordoñez, D Thorne, D Dillon… - Regulatory Toxicology and Pharmacology…, 2015
... Nuclei were visualised using a fluorescence microscope (20× magnification). Where possible, 100 nuclei per slide were scored and DNA damage determined using Comet Assay IV image analysis software (Perceptive Instruments, Harverhill, UK). ...

IRS-1 gene polymorphism and DNA damage in pregnant women with diabetes or mild gestational hyperglycemia
RB Gelaleti, DC Damasceno, DMF Salvadori… - Diabetology and Metabolic Syndrome …, 2015
... were stained with ethidium bromide (20 µg/ml in distilled H2O; 50 µl/slide), and analyzed in afluorescence microscope connected to a charge-coupled device (CCD) camera and a personal computer-based analysis system (Comet Assay IV, Perceptive Instruments, UK) in order ...

Results of the International Validation of the in vivo rodent alkaline comet assay for the detection of genotoxic carcinogens
H Takasawa, R Takashima, K Narumi, K Kawasako… - Mutation Research/Genetic Toxicology …, 2015
... Comet images were observed under a fluorescence microscope at 200× magnification and analyzed using a comet image analyzer (Comet Assay IV, Perceptive Instruments Ltd., Suffolk, UK) connected to a microscope via a charge-coupled device (CCD) camera. ...

Alkaline comet assay in liver and stomach, and micronucleus assay in bone marrow from rats... 
AR Kraynak, JE Barnum, CL Cunningham, A Ng… - Mutation Research/Genetic Toxicology …, 2015
... Comet Assay IV Image Analysis Software (Perceptive Instruments, Haverhill, UK) coupled to a monochrome video camera (Marlin MF-046B, Allied Vision Technologies, Stadtroda, Germany) and a fluorescence microscope (Olympus model BH-2) was used to score slides. ...

Evaluation of p-phenylenediamine, o-phenylphenol sodium salt, and 2,4-diaminotoluene in the rat comet assay as part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiated international validation study of in vivo rat alkaline comet assay 
M de Boeck, K de Vlieger, H Geys, A Vynckier… - Mutation Research/Genetic Toxicology …, 2015
... Fifty randomly selected, non-overlapping nuclei per replicate (ie, 100 nuclei per animal) were scored for DNA migration using the semi-automated image analysis system comet assay IV, v.4.11, from Perceptive Instruments Ltd. ...

In Vitro Evaluation of Genotoxic Effects under Magnetic Resonant Coupling Wireless Power Transfer 
K Mizuno, N Shinohara, J Miyakoshi - … Journal of Environmental Research and Public Health…, 2015
.... Page 5. Int. J. Environ. Res. Public Health 2015, 12 3857 DNA strand breaks were analyzed using a Comet Assay IV (Perceptive Instruments, Bury St. Edmunds, UK), which includes software for calculation of DNA damage from images obtained in the comet assay. ...

Investigation of sodium arsenite, thioacetamide and diethanolamine in the alkaline comet assay: part of the JaCVAM comet validation exerciser 
K C Beevers, D Henderson, L Lillford - Mutation Research/Genetic Toxicology and …, 2015
... Slides were stained with 100 µL of 2 µg/mL ethidium bromide and coverslipped, prior to scoring by fluorescence microscopy, x200 magnification, 510-560 nm excitation filter, 590 nm barrier filter using Perceptive Instruments' (Suffolk, UK) 'Comet Assay IV' image analysis system ...

Inhibitors of the apurinic/apyrimidinic endonuclease 1 (APE1)/nucleophosmin (NPM1) interaction that display anti-tumor properties 
M Poletto, MC Malfatti, D Dorjsuren, PL Scognamiglio… - Molecular Carcinogenesis, 2015
... The analysis of the comet tail was carried out using the Comet Assay IV software (Perceptive Instruments, Suffolk, UK) to determine the Olive tail moment. At least 100 cells per experimental condition were scored. rRNA Maturation Kinetics. ...

Teratogenicity, genotoxicity and oxidative stress in zebrafish embryos (Danio rerio) co-exposed to arsenic and atrazine 
JA Adeyemi, A da Cunha Martins-Junior, F Barbosa - Comparative Biochemistry and Physiology…, 2015
... Comets were evaluated by means of image analysis software Comet Assay IV, version 4.3 (Perceptive Instruments Ltd.), which analyzed the tail intensity parameters, related to the percentage of DNA in the tail (% DNA). Thiobarbituric acid reactive substances (TBARS) Assay. ...

The fruit extract of Berberis crataegina DC: exerts potent antioxidant activity and protects DNA integrity 
M Charehsaz, H Sipahi, E Celep, A Üstündag… - DARU Journal of Pharmaceutical Sciences…, 2015
.... Then the slides were viewed using a fluorescence microscope (Leica DM1000) equipped with an excitation filter of 515-560 nm. A single scorer randomly selected and captured 100 cells using the Perceptive Instruments COMET Assay IV analysis system. ...

In vitro neurotoxicity evaluation of piperazine designer drugs in differentiated human neuroblastoma SH-SY5Y cells 
MD Arbo, R Silva, DJ Barbosa, DD Silva, SP Silva… -Journal of Applied Toxicology…, 2015
... overnight. The dried slides were stained with SYBR ® Gold and 100 cells (50 cells per gel) were scored using the semi-automated image analysis system Comet Assay IV (Perceptive Instruments, Bury St Edmunds, UK). Microscopic ...

Modified Procedure To Assess Dna Breakage In Spermatozoa By Means Of The Comet Assay 
G Martínez-Luna, J Castillo-Cadena...- Rev. Int. Contam. Ambie... 2015
... green light) exciting filter. Two slides were prepared for each treatment. One hundred randomly selected cells were scored by means of the Comet Assay IV Analyzer (Perceptive Instruments Inc.). DNA breakage was evaluated ...

Comparative Proteomic Analysis of the Molecular Responses of Mouse Macrophages to Titanium Dioxide and Copper Oxide Nanoparticles Unravels Some Toxic Mechanisms for Copper Oxide Nanoparticles in Macrophages 
S Triboulet, C Aude-Garcia, L Armand, V Collin-Faure..- PLOS One 2015
... a 456 nm emission filter at ×20 magnification. Percentage of tail DNA was measured by using Comet IV software (Perceptive Instruments, Suffolk, UK). Proteomics. Sample preparation, 2D gel analysis and mass spectrometry ...

Oxidative stress, genotoxicity and histopathology biomarker responses in Mugil cephalus and Dicentrarchus labrax gill exposed to persistent pollutants. A field study in the Bizerte Lagoon: Tunisia
WB Ameur, Y El Megdiche, J de Lapuente, B Barhoumi… - Chemosphere, 2015
... Percent tail DNA was measured using the Comet Assay IV software (Perceptive Instruments, Suffolk, UK). 2.7. Gill histopathology. The gill samples fixed in formaldehyde solution were dehydrated in a graded series of ethanol bathes and embedded in paraffin. ...

Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil
R Martinovic, S Kolarevic, M Kracun-Kolarevic, J Kostic… - Marine Environmental …, 2015
... The slides were examined with a fluorescence microscope (Leica, DMLS, Austria, under magnification 400 X, excitation filter 510-560 nm, barrier filter 590 nm). Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). ...

Optimal dose selection of N-methyl-N-nitrosourea for the rat comet assay to evaluate DNA damage in organs with different susceptibility to cytotoxicity
S Kitamoto, R Matsuyama, Y Uematsu, K Ogata, M Ota… - Mutation Research/Genetic …, 2015
... 20× objective. The images were imported to a computer through a CCD camera attached to the microscope and analyzed using a comet assay analyzer (Comet Assay IV system, Perceptive Instruments, Suffolk, UK). The percentage ...

Genotoxicity evaluation of benzene, di (2-ethylhexyl) phthalate, and trisodium ethylenediamine tetraacetic acid monohydrate using a combined rat comet/ …
S Kitamoto, R Matsuyama, Y Uematsu, K Ogata, M Ota… - Mutation Research/Genetic …, 2015
... magnification 200×). The images were imported into a computer through a CCD camera attached to the microscope and analyzed by a comet assay analyzer (Comet Assay IV System, Perceptive Instruments, Suffolk, UK). The ...

p-Chloroaniline, t-butylhydroquinone, and methyl carbamate: Rat in vivo comet test, JaCVAM trial phase 4.2
W Barfield, B Burlinson - Mutation Research/Genetic Toxicology and …, 2015
... microscope. The comet images from the microscope were projected onto a computer display screen via a CCD camera and measured using Perceptive Instruments COMET IV™ image analysis (Bury St. Edmunds, UK). The ...

The clastogenicity of 4NQO is cell-type dependent and linked to cytotoxicity, length of exposure and p53 proficiency
K Brüsehafer, BB Manshian, AT Doherty, ZM Zaïr… - Mutagenesis, 2015
... each time at room temperature. Finally, the slides were stained with 60-µl propidium iodide (20 µg/ml) for 30min, before scoring using a Comet IV capture system (Perceptive Instruments Ltd., Haverhill, UK). Fifty nuclei per gel ...

A case-control study of genotoxicity endpoints in patients with papillary thyroid cancer
G Marko, J Renato, Š Božena, GV Vera - Mutation Research/Genetic Toxicology and …, 2015
... The slides were then washed with tris buffer, stained with ethidium bromide (10 µg mL -1 ) and stored at 4 °C prior to analysis. Hundred randomly captured nuclei per slide were analysed using comet assay IV (Perceptive Instruments Ltd., UK) image analysis software. ...

Proposal to optimize ecotoxicological evaluation of wastewater treated by conventional biological and ozonation processes
A Wigh, A Devaux, V Brosselin, A Gonzalez-Ospina… - Environmental Science and Pollution Research, 2015
... Slides were coated with 25 µL of a 0.02 % ethidium bromide solution. Each slide was examined through a fluorescence microscope (Axioskop 40, Zeiss Ltd.). Image analysis was performed with the Comet 4.0 software (Perceptive Instruments Ltd.).

Evaluation of Safety of Arrabidaea chica Verlot (Bignoniaceae), a Plant with Healing Properties
TF Gemelli, LS Prado, FS Santos, AP de Souza… - Journal of Toxicology and Pharmacology, 2015
… before scoring using a Comet IV capture system (Perceptive Instruments Ltd., Haverhill, UK). Fifty nuclei per gel were scored and the tail intensity that is defined as the percentage of DNA migrated from the head of the comet into the tail was measured for each nucleus scored …

Geosmin induces genomic instability in the mammalian cell microplate-based comet assay
AF Silva, M Lehmann, RR Dihl - Environmental Science and Pollution Research, 2015
... The stained nucleoids were immediately evaluated at ×400 mag- nification under an Olympus (BX41) fluorescence microscope (excitation filter, 515–560 nm; barrier filter, 590 nm), equipped with an image analysis system (Comet Assay IV, Perceptive Instruments, UK) . ...

Comparison of integrated genotoxicity endpoints in rats after acute and subchronic oral doses of 4-nitroquinoline-1-oxide
DJ Roberts, M McKeon, Y Xu, LF Stankowski - Environmental and Molecular Mutagenesis, 2015
... Neutralized slides were dried and stored at room temperature prior to staining DNA with 20µg/mL ethidium bromide and acquiring data using fluorescence microscopy and an image analysis system (Comet Assay IV; Perceptive Instruments ; Haverhill, UK). ...

DNA protective and antioxidative effects of melatonin in streptozotocin-induced diabetic rats
Sekkin S, Ipek ED, Boyacioglu M, Kum C, Karademir U, Yalinkilinc HS, Ak MO and Basaloglu H, Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop
... moment of comets were made for 100 randomly selected cells, that is, 50 cells from each of two gels from each sample, using a computer-based image analysis system (Comet Assay IV, Perceptive Instruments, Bury St Edmunds, UK). The mean value of the % Tail DNA and ...

Comparative study of genotoxic, antigenotoxic and cytotoxic activities of monoterpenes camphor, eucalyptol and thujone in bacteria and mammalian cells
B Nikolic, B Vasilijevic, D Mitic-culafic, B Vukovic-gacic… - Chemico-Biological Interactions, 2015
... The comets were visualized using fluorescence microscope (Leica, DMLS, Austria) with an excitation filter 510-560 nm, barrier filter 590 nm, at 400x magnification. Image analysis software (Comet Assay IV, Perceptive Instruments, UK) was used for comet analysis. ...

External gamma irradiation-induced effects in early-life stages of zebrafish, Danio rerio
B Gagnaire, I Cavalié, S Pereira, M Floriani, N Dubourg… - Aquatic Toxicology, 2015
... One hundred nucleoids per slide were analysed at x400 magnification under a fluorescence microscope (Nikon Eclipse E600) equipped with a 515 to 560 nm excitation filter. Comet pictures were analysed using Comet IV software (Perceptive Instruments) ...

In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells
G Kiliç, C Costa, N Fernández-Bertólez, E Pásaro… - Toxicology Research, 2015
... 35 with minor modifications 36. Comet IV Software (Perceptive Instruments) was used for image capture and analysis. In all cases 100 cells were scored (50 from each replicate slide), and percentage of DNA in the comet tail (%tDNA) was used as DNA damage parameter. ...

Field evidence of reproduction impairment through sperm DNA damage in the fish nase (Chondrostoma nasus) in anthropized hydrosystems
A Devaux, S Bony, S Plenet, P Sagnes, S Segura… - Aquatic Toxicology, 2015
... et al. (1988). DNA damage was scored using an Axioskop epifluorescence microscope (Zeiss, Germany) equipped with the Comet assay IV image analysis system (Perceptive Instruments Ltd., UK). Randomly selected cells ...

Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay
M Kracun-Kolarevic, S Kolarevic, A Atanackovic… - Water, Air, and Soil Pollution 2015
... The slides were analyzed with a fluorescence microscope (Leica, DMLS, Austria, under magnification ×400, excitation filter 510–560 nm, barrier filter 590 nm) linked to an image analysis Comet IV Computer Software (Perceptive Instruments, UK). ...

Characterising cytotoxicity and genotoxicity of chemotherapeutic agents (Etoposide and Vincristine) on TK6, a human lymphoblastoid cell line
L Edermaniger - The Plymouth Student Scientist, 2015
... bias. The slides were viewed under a Texas Red Filter with images collected containing 50-100 cells per treatment. The Perceptive Instruments Comet Assay IV software was used to measure DNA damage. Micronucleus assay ...

Impact of a synthetic cannabinoid (CP-47,497-C8) on protein expression in human cells: evidence for induction of inflammation and DNA damage
A Bileck, F Ferk, H Al-Serori, VJ Koller, B Muqaku… - Archives of Toxicology, 2015
... Air-dried slides were stained with propidium iodide (10.0 µg/mL, Sigma-Aldrich, Steinheim, Germany). The percentage of DNA in tail was measured by use of a computer-aided image analysis system (Comet IV, Perceptive Instruments Ltd., Haverhill, UK). ...

Consumption of a dark roast coffee decreases the level of spontaneous DNA strand breaks: a randomized controlled trial
T. Bakuradze, R. Lang, T. Hofmann, G. Eisenbrand, D. Schipp, J. Galan and E Richling - European Journal of Nutrition, 2015
... Slides were analyzed by computerized image analysis (Comet IV, Perceptive Instruments), scoring 2 9 50 images per slide (2 gels per slide). DNA migration was directly expressed as mean tail intensity (TI %) from 2 gels. Data are presented as spontaneous DNA strand breaks. ...

Primary and oxidative DNA damage in salivary leukocytes as a tool for the evaluation of air pollution early biological effects in children: current status of the MAPEC (Monitoring Air Pollution Effects on Children for supporting public health policy) study.
S Vannini, S Levorato, E Ceretti, M Villarini, S Bonetta… Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop.
... DNA damage was measured using the tail intensity (% DNA) parameter (Comet Assay IV, Perceptive Instruments, UK. ... DNA damage was measured using the tail intensity (%DNA) parameter (Comet Assay IV, Perceptive Instruments, UK. ...

Micronutrients intake associated with DNA damage assessed by in a human biomonitoring study
C Ladeira, S Viegas, E Carolino, MC Gomes, M Brito..Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop.
... Percentage of DNA in the tail and oxidative damage parameters were measured using Zeiss AxioScope.A1 fluorescence microscope and Comet Assay IV capture system (Perceptive Instruments software) and 50 nucleoids were scored per gel. ...

Comet assay as a human biomonitoring tool: application in occupational exposure to antineoplastic drugs
C Ladeira, S Viegas, M Pádua, E Carolino, MC Gomes… Front. Genet. Conference Abstract: ICAW 2015 - 11th International Comet Assay Workshop.
... Percentage of DNA in the tail and oxidative damage parameters were measured using Zeiss AxioScope.A1 fluorescence microscope and Comet Assay IV capture system (Perceptive Instruments software) and 50 nucleoids were scored per gel. ...

Ultra high pressure homogenisation of almond milk: Physico-chemical and physiological effects
K Briviba, V Gräf, E Walz, B Guamis, P Butz - Food Chemistry, 2015.
... The percentage of fluorescence in the tail (tail intensity,%) was assessed fluorimetrically (DM 400 B, Leica Microsystems) and then quantified using the imaging software of Perceptive Instruments (Halstead, UK). Results are given as mean ± SD. 2.10. Statistical analysis. ...

Evaluation of 4, 4'-diaminodiphenyl ether in the rat comet assay: Part of the Japanese Center for the Validation of Alternative Methods (JaCVAM)-initiative …
CC Priestley, JS Walker, MR O'Donovan, A Doherty - Mutation Research/Genetic …, 2015
... Twenty minutes later slides were scored, 50 nuclei per gel, using a Comet IV capture system (Perceptive Instruments). Tail intensity (TI) was used as the measure of damage and was defined as the percentage of DNA that had migrated from the head of the comet into the tail. ...

Toxicity of diuron in human cancer cells
M Huovinen, J Loikkanen, J Naarala, K Vähäkangas - Toxicology in Vitro, 2015
... were analyzed. The analysis was performed by fluorescence microscopy (Axio Imager.A1, Carl Zeiss, Göttingen, Germany) using the Comet Assay IV (Perceptive Instruments, Haverhill, UK) image analysis system. The olive ...

Cytotoxic and genotoxic potencies of single and combined spore extracts of airborne OTA-producing and OTA-non-producing Aspergilli in Human lung A549 cells
MŠ Klaric, DJ Despot, N Kopjar, D Rašic, S Kocsubé… - Ecotoxicology and Environmental Safety, 2015
... For image analysis, DNA was stained with 100–250 µL ethidium bromide solution (20 µg/mL) per slide for 10 min. Slides were scored using an image analysis system (Comet assay IV, Perceptive instruments Ltd., UK) connected to a fluorescence microscope (Zeiss, Germany). ...

Detection of Cyto-and Genotoxicity of Rod-Shaped Gold Nanoparticles in Human Blood Lymphocytes Using Comet-FISH
JP Soares, AM Silva, MM Oliveira, F Peixoto, I Gaivão… - AGE, 2015
... Slides were stained with ethidium bromide (20 µg/mL) immediately before visualization of comet DNA using a Nikon Eclipse E400. DNA damage estimation was evaluated by the image analysis software Comet IV (Perceptive Instruments, Ltd.) (Fig. ...

Effects of combined physical exercise training on DNA damage and repair capacity: role of oxidative stress changes
JP Soares, AM Silva, MM Oliveira, F Peixoto, I Gaivão… - AGE, 2015
... Slides were stained with ethidium bromide (20 µg/mL) immediately before visualization of comet DNA using a Nikon Eclipse E400. DNA damage estimation was evaluated by the image analysis software Comet IV (Perceptive Instruments, Ltd.) (Fig. ...

Study of gaseous benzene effects upon A549 lung epithelial cells using a novel exposure system
M Mascelloni, JM Delgado-Saborit, NJ Hodges… - Toxicology Letters, 2015
.. 590 nm. A USB digital camera (Merlin, Allied Vision Technologies, UK) received the images, which were analysed using a personal computer-based image analysis system Comet Assay IV (Perceptive instruments, UK). A random ...

Genotoxicity of three food processing contaminants in transgenic mice expressing human sulfotransferases 1A1 and 1A2 as assessed by the in vivo alkaline single cell gel electrophoresis assay
AH Høie, C Svendsen, G Brunborg, H Glatt… - …Environmental and Molecular Mutagenesis, 2015
... Scoring was performed using the Comet Assay IV software (Perceptive Instruments, Bury St. Edmunds, UK). ...

Increased DNA Damage is Related to Maternal Blood Glucose Levels in the Offspring of Women With Diabetes and Mild Gestational Hyperglycemia
RB Gelaleti, DC Damasceno, DP Santos, IMP Calderon… - Reproductive Sciences, 2015
... stained with ethidium bromide (20 µg/mL in distilled water; 50 µL/slide) and analyzed under a fluorescence microscope connected to a charge-coupled device camera and a personal computer-based analysis system (Comet Assay IV, Perceptive Instruments, United Kingdom). ...

Dysregulation of markers of oxidative stress and DNA damage among nail technicians despite low exposure to volatile organic compounds.
P Grešner, M Stepnik, MB Król, R Swiercz… - Scandinavian journal of …, 2015
... Finally, the slides were stained with 5 µg/ml DAPI and 50 cells from each slide were analyzed using an Olympus fluorescence microscope (a BX40 instrument; Olympus, Tokyo, Japan) equipped with an image analy- sis system (Comet IV, Perceptive Instruments, UK). ...

Methyleugenol and oxidative metabolites induce DNA damage and interact with human topoisomerases
IAM Groh, O Rudakovski, M Gründken, A Schroeter… - Archives of Toxicology, 2015
... Comet analy- sis was performed using a Zeiss Axioskop (Carl Zeiss AG, Göttingen, Germany) and the image analysis software Comet Assay IV (Perceptive Instruments, Suffolk, UK). Tail intensity is specified as DNA signals in the comet head ...

Further characterization of benzo [a] pyrene diolepoxide (BPDE)-induced comet assay effects
J Bausinger, P Schütz, AL Piberger, G Speit - Mutagenesis, 2015
... Images of 100 randomly selected cells stained with ethidium bromide were analysed from each coded slide by image analysis (Comet Assay IV, Perceptive Instruments, Haverhill, UK) and the mean tail intensity (TI; % tail DNA) was evaluated as a measure for DNA migration. ...

Comparative study of curcumin and curcumin formulated in a solid dispersion: Evaluation of their antigenotoxic effects
LM Mendonça, CS Machado, CCC Teixeira… - Genetics and Molecular …, 2015
... Tail intensity (% tail DNA) was evaluated using the Comet Assay IV software (Perceptive Instruments, Suffolk, UK). Micronucleus test. The micronucleus test was performed according to the protocol described by Schmid (1975) . ...

Effect of Allium flavum L. and Allium melanantherum Panc. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase
D Mitic-Culafic, B Nikolic, N Simin, N Jasnic… - Plant Foods for Human …, 2015
... ethidium bromide (5 µg mL -1 ) and visualized using fluorescence microscope (Leica, DMLS, Austria) with an excitation filter 510–560 nm, barrier filter 590 nm, mag- nification 400 x. Image analysis software (Comet Assay IV, Version 4.2, 2007, Perceptive Instruments, UK) was ...

In vitro and in vivo genotoxicity investigations of differently sized amorphous SiO 2 nanomaterials
E Maser, M Schulz, UG Sauer, M Wiemann, L Ma-Hock… - Mutation Research/Genetic …, 2015
...Comet images were evaluated and scored with a charge-coupled device (CCD) camera by image analysis using the Comet Assay IV(Perceptive Instruments Ltd., Suffolk, UK)...

Survivin contributes to DNA repair by homologous recombination in breast cancer cells
E Véquaud, G Desplanques, P Jézéquel, P Juin… - Breast Cancer Research …, 2015
... The comet assay was performed in alkaline or neutral buffer, as previously described [22]. CometSlideTM was visualized using a Leica microscope, and images were analyzed into Comet Assay IV software (Perceptive instruments, Suffolk, UK). ...

Genotoxicity assessment of propyl thiosulfinate oxide, an organosulfur compound from Allium extract, intended to food active packaging
P Mellado-García, S Maisanaba, M Puerto… - Food and Chemical …, 2015
... (2014b). Finally, DNA was visualised (Olympus BX61 fluorescence microscope) and at least 100 randomly selected nuclei per concentration were analysed using image analysis software (Comet Assay IV, Perceptive Instruments, UK). ...

Colon Cancer Chemoprevention by Sage Tea Drinking: Decreased DNA Damage and Cell Proliferation
DFN Pedro, AA Ramos, CF Lima… - Phytotherapy 2015
... To each slide, 20 µL of ethidium bromide was added, and 100 cells were counted under a fluorescent microscope and quantified by visual scoring and using Comet 4 image analysis system (Perceptive Instruments). Statistical analysis. ...

In vitro comet and micronucleus assays do not predict morphological transforming effects of silica particles in Syrian Hamster Embryo cells.
C Darne, C Coulais, F Terzetti, C Fontana… -Genetic Toxicology and Environmental Mutagensis 2016
... The slides were then washed with Tris–HCl 0.4 M for 15 min and stained with propidium iodide. Images of 100 randomly selected comets were analysed from each sample, using specific image analyser software (Comet assay IV, Perceptive Instruments, United Kingdom ...

The Caenorhabditis elegans Werner syndrome protein participates in DNA damage checkpoint and DNA repair in response to CPT-induced double-strand breaks
M Hyun, S Choi, T Stevnsner, B Ahn - Cellular Signalling, 2015
... Observations were performed under an epifluorescence microscope (Carl Zeiss Axioskop2 plus). The tail lengths of 100–150 nuclei were measured using a Comet Assay IV (Perceptive Instruments, Ltd.). 3. Results. 3.1. wrn-1 (gk99) mutants are sensitive to CPT. ...

Sperm DNA quality evaluated by Comet assay and SCSA in stallions following unilateral orchiectomy
R Serafini, DD Varner, W Bissett, TL Blanchard… - Theriogenology, 2015
... incubated for 5 min in the dark and scored with a fluorescent microscope (Olympus BX51; Olympus America, Inc., Melville, NY, USA) at magnification X 200, connected to a computer with Image Analysis Software, Comet Assay IV Version 4.11

Cyto-and genotoxic effects of metallic nanoparticles in untransformed human fibroblast
LP Franchi, BB Manshian, TAJ de Souza, SJ Soenen… - Toxicology in Vitro, 2015
... For each slide 50 nucleoids (total 100 per sample) were scored by the Comet Assay IV® software (Perceptive Instruments Ltd., Suffolk, UK), the tail intensity (% DNA in tail) was used as the geno-toxicity parameter. Three independent experiments were performed. 2.11. ...

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