Papers from 2016

Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3
S Kolarevic, M Kracun-Kolarevic, J Kostic, J Slobodnik… - Science of The Total Environment, 2016
... The slides were examined with a fluorescence microscope (Leica, DMLS, Austria, under magnification 400 ×, excitation filter 510–560 nm, barrier filter 590 nm). Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). ...

In vitro comet and micronucleus assays do not predict morphological transforming effects of silica particles in Syrian Hamster Embryo cells.
C Darne, C Coulais, F Terzetti, C Fontana… -Genetic Toxicology and Environmental Mutagensis 2016
... The slides were then washed with Tris–HCl 0.4 M for 15 min and stained with propidium iodide. Images of 100 randomly selected comets were analysed from each sample, using specific image analyser software (Comet assay IV, Perceptive Instruments, United Kingdom ...

Genotoxicity of Water Contaminants from the Basin of Lake Sevan, Armenia Evaluated by the Comet Assay in Gibel Carp (Carassius auratus gibelio) and Tradescantia Bioassays
A Simonyan, B Gabrielyan, S Minasyan… - Bulletin of Environmental Contamination and Toxicology, 2016
... The images were evaluated by fluorescence microscope (ZEISS, Germany). DNA damage was assessed using a Comet Assay IV imaging system (Perceptive Instruments, UK). A total of 150 cells were analyzed in three replicated slides from each fish. ...

Long-term exposure of A549 cells to titanium dioxide nanoparticles induces DNA damage and sensitizes cells towards genotoxic agents
L Armand, A Tarantini, D Beal, M Biola-Clier… - Nanotoxicology, 2016
... charge-coupled device camera with a 350-390 nm excitation and 456 nm emission filter, at x20 magnification. Comets were measured and analyzed using Comet IV software (Perceptive Instruments, Suffolk, UK). To evaluate DNA ...

Water browning mediates predatory decimation of the Arctic fairy shrimp Branchinecta paludosa
M Lindholm, R Wolf, A Finstad… - Freshwater Biology, 2016
... Comets (ie distributions of DNA fragments on the gel) were scored on a Nikon Eclipse LV 100ND microscope (Nikon Corporation, Tokyo, Japan) with a Nikon Plan Fluor 20x/0.50 objective (Nikon Corporation) using Comet Assay IV software (version 4.3; Perceptive Instruments, ...

Anticancer activity and radiosensitization effect of methyleneisoxazolidin-5-ones in hepatocellular carcinoma HepG2 cells
K Gach, I Gradzka, I Wasyk… - Chemico-Biological Interactions, 2016
... The pictures of a 100 randomly selected comets per slide were captured at 200 x magnification using a fluorescent Labophot-2 microscope (Nicon, Japan). Image analysis of the data was performed by the Comet Assay IV Image Analysis System (Perceptive Instruments ...

Leccinum vulpinum Watling induces DNA damage, decreases cell proliferation and induces apoptosis on the human MCF-7 breast cancer cell line
FS Reis, D Sousa, L Barros, A Martins… - Food and Chemical Toxicology, 2016
...Comets were visualized using the fluorescent microscope mentioned above and representative photographs were taken. The analysis was made using the 'Comet Assay IV v4.3' imaging system (Perceptive Instruments). A minimum of 100 cells per condition were ...

The Brassica-derived phytochemical indolo[3,2-b]carbazole protects against oxidative DNA damage by aryl hydrocarbon receptor activation
D Faust, T Nikolova, W Wätjen, B Kaina… -Genotoxicity And Carcinogenicity, Archives of Toxicology, 2016
... Comets were analysed by fluorescence microscopy using an Olym- pus BX50 equipped with a ColorView camera (Olympus, Münster, Germany). At least 50 cells/slide were scored using the Comet IV software (Perceptive Instruments Ltd., Bury St Edmunds, UK). ...

Chemical characterization and in vitro cyto- and genotoxicity of ‘legal high’ products containing Kratom (Mitragyna speciosa)
AS Oliveira, S Fraga, F Carvalho, AM Araújo… - Forensic Toxicology, 2016
...The slides were coded and one scorer performed the analysis of comets using a fluorescence microscope and the image analysis software Comet Assay IV (Perceptive Instruments, Suffolk, UK). The percentage ...

Vitamin D3 Deficiency Increases DNA Damage and the Oxidative Burst Of Neutrophils in a Hypertensive Rat Model
C da Silva Machado, VP Venancio, AF Aissa… - Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2016
... Tail intensity (equivalent to % tail DNA) was evaluated using the Comet Assay IV ® software (Perceptive Instruments, Suffolk, UK), and the results are presented as the mean value per group. 2.6. Micronucleus test in bone marrow and peripheral blood cells. ...

Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B
CD Major, RS Derbes, H Wang, AM Roy-Engel - Gene Reports, 2016
... The digital images, acquired with a SensiCam QE digital camera (Cooke Corporation, Romulus, MI), was used to score tail moments in at least 75 random cells using the Comet Assay IV software (Perceptive Instruments, United Kingdom). ...

Does 2,4-dichlorophenoxyacetic acid (2,4-D) induce genotoxic effects in tissue cultured Allium roots?
M Özkul, ÇA Özel, D Yüzbasioglu, F Ünal - Cytotechnology, 2016
...The comet analysis was performed on randomly selected 25 nuclei by using specialized image analysis system (Comet Assay IV, Perceptive Instruments Ltd., UK). Statistical analysis. The data obtained were ...

Arabidopsis thaliana siRNA biogenesis mutants have the lower frequency of homologous recombination
Y Yao, A Bilichak, A Golubov… - Plant signaling and Behavior, 2016
...The length of comet tails was evaluated with the Comet Assay IV software system (Perceptive Instruments Ltd.). Two independent biological replicas with two technical repeats were performed. Over 100 ... ..

Genotoxicity of monosodium glutamate
N Ataseven, D Yüzbasioglu, AÇ Keskin… - Food and Chemical Toxicology, 2016
... The tail lenght (µm), tail intensity (%) and tail moment of 100 comets on each slide (a total of 300 comets for each concentration) were determined, using specialized Image Analysis System (“Comet Assay IV”, Perceptive Instruments Ltd., UK). 2.8. Statistical analysis. ...

DNA damage in hemodialysis patients with chronic kidney disease; a test of the role of diabetes mellitus; a comet assay investigation
S Mamur, F Unal, K Altok, SM Deger… - Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2016
... Two replicate slides were prepared for each individual. The tail length and tail intensity of 100 comets per individual were determined using a specialized Image Analysis System (“Comet Assay IV”, Perceptive Instruments, Haverhill, UK). 2.6. Statistical analysis. ...

Genotoxicity of a thiosulfonate compound derived from Allium sp. intended to be used in active food packaging: In vivo comet assay and micronucleus test
P Mellado-García, M Puerto, AI Prieto… - Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2016
... system (DP controller-DP manager). Images of at least 100 randomly selected nuclei per animal were analyzed with the image analysis software Comet Assay IV (Perceptive Instruments, UK). Percentages of tail DNA (% DNA ...

Cytotoxic and genotoxic activities of waters and sediments from highway and parking lot runoffs
SJ Jung, YJ Choi, NN Kim, JY Choi, BS Kim… - Fish & shellfish …, 2016
... of DNA migration from head), percentage of DNA in tail (tail intensity/total intensity in tail), and tail moment (amount of DNA damage, product of tail length, and percentage of DNA in tail) using comet assay IV image analysis software (version 4.3.2, Perceptive Instruments ...

Cellular Effects in an In Vitro Human 3D Cellular Airway Model and A549/BEAS-2B In Vitro Cell Cultures Following Air Exposure to Cerium Oxide Particles at an Air–Liquid Interface
IM Kooter, M Gröllers-Mulderij… - Applied In Vitro Toxicology, 2016
... dried. For scoring, each slide was stained with ethidium bromide. Fifty randomly chosen cells were analyzed per slide (Comet Assay IV; Perceptive Instruments Ltd.). In total 300 cells per treatment concentration were evaluated. ... ...

Assessment of status of three water bodies in Serbia based on tissue metal and metalloid concentration (ICP-OES) and genotoxicity (comet assay)
K Sunjog, S Kolarevic, M Kracun-Kolarevic… - Environmental Pollution, 2016
... genotoxic effects in blood, liver and gills of the European chub. Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). The objective of our study was to investigate two reservoirs ...

Cytotoxic and genotoxic potential of Cr(VI), Cr(III)-nitrate and Cr(III)-EDTA complex in human hepatoma (HepG2) cell
SJ Jung, YJ Choi, NN Kim, JY Choi, BS Kim… - Fish & shellfish …, 2016
...The slides were stained with ethidium bromide (5 µg mL -1 ) and images of 50 randomly selected nuclei per experimental point were analyzed with image analysis software Comet Assay IV (Perceptive Instruments ...

Human mesenchymal stem cells are resistant to cytotoxic and genotoxic effects of cisplatin in vitro
K BC Bellagamba, BRR Abreu, I Grivicich…- Genetics and Molecular Biology, 2016
...After coding and blinding of the slides, they were stained with ethidium bromide solution and the comets were determined by an image analysis system (Comet Assay IV, Perceptive Instruments, UK). Four slides ...

Genotoxicity assessment of intravenously injected titanium dioxide nanoparticles in gpt delta transgenic mice
T Suzuki, N Miura, R Hojo, Y Yanagiba, M Suda… - Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2016
...The slides were stained with ethidium bromide (5 µg mL -1 ) and images of 50 randomly selected nuclei per experimental point were analyzed with image analysis software Comet Assay IV (Perceptive Instruments ...

Evaluation of genotoxicity of general anesthesia maintained with desflurane in patients under minor surgery
FR Nogueira, LG Braz, LR de Andrade… - Environmental and Molecular Mutagenesis, 2016
... fixed with absolute ethanol and stored at 4°C. Prior to analysis, the slides were stained with SYBR Gold and immediately analyzed using a fluorescent microscope, at a magnification of 400×, connected to an image analysis system (Comet Assay IV, Perceptive ...

Protective and Antigenotoxic Effects of Silymarin and Curcumin in Experimental Cyclophosphamide Intoxication in Rats
H AVCI, S SEKKIN, M BOYACIOGLU, H AKSIT - Kafkas Univ Vet Fak Derg, 2016
... Electrophoresis (CS - 300V, Cleaver Scientific, UK) was carried out at approximately 1 V/cm for 20 min. Measurements of the tail intensity and tail moment of comets were made using a computer-based image analysis system (Comet Assay IV, Perceptive Instruments, UK). ...

Genetic and rat toxicity studies of cyclodextrin glucanotransferase
RR Maronpot, CA Hobbs, J Davis, C Swartz, M Boyle… - Toxicology Reports, 2016
... Following a preliminary incubation at 37 °C for 20 min prior to plating, three test plates per concentration were incubated at 37 °C for 48 h and then counted using the Sorcerer/Ames Study Manager System (Perceptive Instruments, Ltd., Suffolk, UK) ... … 00 cells were scored per sample at 200x magnification without knowledge of sample identity using Comet Assay IV Imaging Software¸ Version 4.3.1 (Perceptive Instruments, Ltd., Suffolk, UK)….

Lagos lagoon sediment organic extracts and polycyclic aromatic hydrocarbons induce embryotoxic, teratogenic and genotoxic effects in Danio rerio (zebrafish) embryos
TO Sogbanmu, E Nagy, DH Phillips, VM Arlt… - Environmental Science and Pollution Research, 2016
... All slides were examined at ×40 magnification using a fluorescent microscope (Leica Microscope, Microsystems Wetzler GmbH, Germany) and equipped with a CCD camera (Marlin imaging device) and analysed using the Comet IV software (2006) (Perceptive Instruments,UK). ...

Evaluation of cytogenetic and DNA damage induced by the antidepressant drug-active ingredients, trazodone and milnacipran, in vitro
E Avuloglu Yilmaz, F Unal, D Yuzbasioglu - Drug and Chemical Toxicology, 2016
... The tail length (µm), tail intensity (%) and tail moment of 100 comets on each slide (a total of 200 comets for each concentration) were determined, using specialized Image Analysis System (Comet Assay IV, Perceptive Instruments Ltd., Haverhill, UK). Statistical analysis. ...

Different sensitivities of cultured mammalian cells towards aphidicolin-enhanced DNA effects in the comet assay
G Speit, P Schütz, J Bausinger - Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2016
... slide. Measurements were made by image analysis (Comet Assay IV, Perceptive Instruments, Haverhill, UK) and DNA migration was determined by measuring the “tail intensity” (% tail DNA). 2.4. Statistical analysis. Pre-experiments ...

Genotoxicity of ferric oxide nanoparticles in Raphanus sativus: Deciphering the role of signaling factors, oxidative stress and cell death
Q Saquib, M Faisal, AA Alatar, AA Al-Khedhairy… - Journal of Environmental Sciences, 2016
... Images from 50 cells (25 from each replicate slide) were randomly scored and subjected to image analysis using software Comet Assay IV (Perceptive Instruments, Suffolk, UK). 1.8. Flow cytometric analysis of apoptosis in radish. ...

DNA damage and oxidative DNA damage in Inflammatory Bowel Disease
C Pereira, R Coelho, D Grácio... Journal of Crohn's and Colitis, 2016
...The Comet Assay IV [Perceptive Instruments, UK] software was used for DNA measurements, and the parameter used to measure DNA damage was % Tail DNA [Figure 1], here referred as 'arbitrary unit' [AU], and recommended for being the most reliable comet assay ...

Ecotoxicological risk assessment of the “Acid Black 210” dye
OP Rocha, CA Cesila, EM Christovam… - Toxicology, 2016
... 0,4 M Tris-HCl, pH 7.5) for 20 min, stained with ethidium bromide (10 µg/mL), examined under a fluorescence microscope (Eclipse, Model E200-LED, Nikon), and counted by the video imaging system Comet Assay IV™ version 4.3 (Perceptive Instruments, United ...

The impact of lymphocyte isolation on induced DNA damage in human blood samples measured by the comet assay
J Bausinger, G Speit - Mutagenesis, 2016
... analysed from each slide. Measurements were made by image analysis (Comet Assay IV, Perceptive Instruments, Haverhill, UK) and DNA migration was determined by measuring the 'tail intensity' (% tail DNA). Statistical analysis. ...

Chronic acrylamide exposure in male mice induces DNA damage to spermatozoa; potential for amelioration by resveratrol
AL Katen, SJ Stanger, AL Anderson, B Nixon… - Reproductive Toxicology, 2016
... Slides were stained with SYBR ® green, covered with a cover slip and visualised under fluorescence. The DNA integrity of 50 cells per slide was analysed using Comet Assay IV software (Perceptive Instruments, Suffolk, UK). ...

Assessment of DNA damage on a group of firefighters
AP Abreu, A Duarte, S Silva, S Morais, MC Pereira… - Environment & Health International, Special Issue, 2016
... to dry overnight. Dried slides were stained with SYBR® Gold and 150 cells (75 cells per gel) were scored using the semi-automated image analysis system Comet Assay IV (Perceptive Instruments, UK). Microscopic analyses ...

Protective effects of the exopolysaccharide Lasiodiplodan against DNA damage and inflammation induced by doxorubicin in rats: cytogenetic and gene expression assays
MB de Melo, C da Silva Machado, DL Ribeiro, AF Aissa… - Toxicology, 2016
... From each sample, two slides were made and from each, 50 cells were evaluated per animal. Comets were scored using the Comet Assay IV software (Perceptive Instruments, Haverhill, England); the percentage of DNA in tail was determined as a parameter of DNA damage. ...

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles
IM Pongrac, I Pavicic, M Milic, LB Ahmed… - International Journal of Nanomedicine, 2016
... were examined at 250× magnification with a fluorescence micro- scope (excitation filter 515–560 nm, barrier filter 590 nm; Zeiss), and DNA-damage parameters were scored using a computer-based image-analysis system (Comet Assay IV; Perceptive Instruments, Bury ...

PKCa and HMGB1 antagonistically control hydrogen peroxide-induced poly-ADP-ribose formation
A Andersson, A Bluwstein, N Kumar, F Teloni… - Nucleic Acids Research, 2016
... DNA damage (double-strand breaks, single-strand breaks, abasic sites) in cells was determined using the Alkaline Comet Assay (Trevigen) according to the manufacturer's instructions. %DNA in tail was calculated using Comet Assay IV (Perceptive Instruments). ...

Early Genotoxic and Cytotoxic Effects of the Toxic Dinoflagellate Prorocentrum lima in the Mussel Mytilus galloprovincialis
AMV Prego-Faraldo, V Valdiglesias, B Laffon, J Mendez… - Toxins, 2016
.... HPLC/MS analyses were performed on a Thermo LTQ Orbitrap instrument (Thermo Fisher ... The alkaline comet assay was performed as described elsewhere [13,44] with slight ... capture and analysis was performed using the Comet IV Software (Perceptive Instruments, Bury St Edmunds . ...

An in vitro study on the genotoxic effect of substituted furans in cells transfected with human metabolizing enzymes: 2, 5-dimethylfuran and furfuryl alcohol
MP Huffman, AH Høie, C Svendsen, G Brunborg… - Mutagenesis, 2016
... of the four replicate gels using an Olympus BX51 fluorescent microscope (Olympus, Tokyo, Japan) connected to a CCD camera (BASLER A312f-VIS, Basler, Ahrensburg, Germany), and employing the Comet Assay IV image analysis system (Perceptive Instruments Ltd., Bury St. Edmunds ...

Report on the rat Pig-a assay using an anti-rat erythroid marker HIS49 antibody in a single dose study of 1, 2-dimethylhydrazine
AS Chikura, Y Okada, T Kimoto, H Kaneko, D Miura… - Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2016
... The visualization and analysis of comets were made using a digital camera linked to an image analyzer system Comet Assay IV (Perceptive Instrument, Suffolk, UK) with a fluorescence microscope BX51 (Olympus, Tokyo, Japan) at a magnification of 200X....

Assessment of oxidative stress responses and the cytotoxic and genotoxic potential of the herbicide tembotrione in HepG2 cells
MS Žunec, V Kašuba, I Pavicic, AM Marjanovic, B Tariba… - Food and Chemical Toxicology, 2016
... Scoring and analysis of slides was performed using the Comet Assay IV analysis system (Perceptive Instruments Ltd., UK), connected with an epifluorescence microscope (Olympus BX51, Tokyo, Japan). A total of 100 comets ...

Silicon Dioxide Impedes Antiviral Response and Causes Genotoxic Insult During Calicivirus Replication
SS Agnihothram, SA Vermudez, L Mullis, TA Townsend… - Journal of Nanoscience and Nanotechnology, 2016
... Error bars represent SEM. **P 0 05 as analyzed by a Students T test. selected randomly from each slide and scored with a Nikon 501 fluorescence microscope and Comet IV digital imag- ing software (Perceptive Instruments, Wiltshire, UK). ...

Alleviation of Aflatoxin B1-Induced Genomic Damage by Proanthocyanidins via Modulation of DNA Repair
SA Bakheet, AM Alhuraishi, NO Al-Harbi… - Journal of Biochemical and Molecular Toxicology, 2016
... On each slide, 100 comets were quantified at × 10 magnification using the Comet Assay IV Image analysis system (Perceptive Instruments, Bury Saint Edmunds, UK) in live-video mode on a Zeiss Axiovert 35 fluorescent microscope (Zeiss). ...

Overexpression of the scaffold WD40 protein WRAP53ß enhances the repair of and cell survival from DNA double-strand breaks
H Rassoolzadeh, S Böhm, E Hedström, H Gad… - Cell Death & Disease, 2016
... On each slide, 100 comets were quantified at × 10 magnification using the Comet Assay IV Image analysis system (Perceptive Instruments, Bury Saint Edmunds, UK) in live-video mode on a Zeiss Axiovert 35 fluorescent microscope (Zeiss). ...

The application of the comet assay to assess the genotoxicity of environmental pollutants in the nematode Caenorhabditis elegans
S Imanikia, F Galea, E Nagy, DH Phillips… - Environmental Toxicology and Pharmacology, 2016
... DNA damage was scored using the Comet IV capture system (version 4.11; Perceptive Instruments, UK). Fifty cell nucleoids were assessed per slide, and each sample was analysed in duplicate. All samples were measured blind. ...

Genotoxicity biomarkers for airborne particulate matter (PM2. 5) in an area under petrochemical influence
AT Lemos, CT de Lemos, AN Flores, EO Pantoja… - Chemosphere, 2016
... and evaluated under a fluorescence microscope. The analysis of 100 cells was performed to calculate tail intensity, using IV Comet Assay software (Perceptive Instruments, Suffolk, UK). The sites sampled were compared to ...

Overexpression of HPV16 E6* Alters ß-Integrin and Mitochondrial Dysfunction Pathways in Cervical Cancer Cells
W EVANS, M FILIPPOVA, V FILIPPOV, S BASHKIROVA… - Cancer Genomics- …, 2016
... One hundred DNA tails were photographed and analyzed on a BIOREVO fluorescence microscope (BZ-9000) (Keyence Corporation, Itasca, IL, USA) with Comet IV software (Perceptive Instruments Ltd., St Frances House, Bury St Edmunds, UK) using uniform magnification ...

Genotoxicity of TiO2 nanoparticles assessed by mini-gel comet assay and micronucleus scoring with flow cytometry
S Di Bucchianico, F Cappellini, F Le Bihanic, Y Zhang… - Mutagenesis, 2016
... 120s before each measurement, and 10 repeated measurements were performed for each sample at 37°C. Cumulants analysis (auto-setting by instrument) was used ... A fluorescence microscope (Leica DMLB) with comet assay IV software (Perceptive Instruments) was used ...

Comet assay: an essential tool in toxicological research
W EVANS, M FILIPPOVA, V FILIPPOV, S BASHKIROVA… - Cancer Genomics- …, 2016
... Analysis was performed after staining with SYBR® Green using the Perceptive Instruments' Comet Assay IV™ software (magnifica- tion: ×40) ... In combination with an image analysis system (Comet Assay IV, Perceptive Instruments), a semiautomated evaluation was possible. ...

Vitamin D and oxidation-induced DNA damage: is there a connection?
EW Wang, AR Collins, MYC Pang, PPM Siu, CKY Lai… - Mutagenesis, 2016
... We used a Leica CTR6500 fluorescent microscope and LAS-AF scanning software (Leica Microsystems Ltd., Wetzlar, Germany) and the Comet Assay IV Lite scoring system (Perceptive Instruments Ltd., Bury St Edmunds, UK). ...

Combining different assays and chemical analysis to characterize the genotoxicity of waters impacted by textile discharges
FI Vacchi, JAS Vendemiatti, V Brosselin… Environmental and Molecular Mutagenesis, 2016 /em>
... 0.02% ethidium bromide solution. Comets were viewed by fluorescence microscopy (Axioskop 40, ZeissLtd). Image analyses were performed with the Comet 4.0 software (Perceptive Instruments). For each concentration, the ...

In Vitro Toxicological Assessment of Cobalt Ferrite Nanoparticles in Several Mammalian Cell Types
M Abudayyak, TA Gurkaynak, G Özhan - Biological Trace Element Research, 2016
... The number of DNA breaks were scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400 magnifica- tion using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Impact of phase I metabolism on uptake, oxidative stress and genotoxicity of the emerging mycotoxin alternariol and its monomethyl ether in esophageal cells
C Tiessen, D Ellmer, H Mikula, G Pahlke, B Warth… - Archives of Toxicology, 2016
... staining with ethidium bromide. Slides were subjected to computer-aided image analysis (Comet Assay IV System, Perceptive Instruments, Suffolk, UK), scoring 2 × 50 randomly picked cells per slide. The results were parameterized ...

Impact of common cytostatics on DNA damage in freshwater mussels Unio pictorum and U. tumidus
S Kolarevic, Z Gacic, J Kostic, K Sunjog… - CLEAN–Soil, Air, Water, 2016
... Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). For in vivo experiments, five slides were prepared for each concentration (one slide per specimen). For in vitro experiments, three ...

Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin
M Kracun-Kolarevic, S Kolarevic, J Jovanovic… - Science of The Total Environment, 2016
... Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). Tail intensity (TI % - percentage of DNA in the tail of the comet) was chosen as a measure of DNA damage. For each sample 100 nucleoids were scored. ...

Investigations of the genotoxic properties of two synthetic cathinones (3-MMC, 4-MEC) which are used as psychoactive drugs
H Al-Serori, F Ferk, V Angerer, M Mišík, A Nersesyan… - Toxicology Research, 2016
... Air-dried slides were stained with propidium iodide (10 µg mL -1 ); subsequently the percentage of DNA in the tails was measured by use of a computer aided image analysis system (Comet IV, Perceptive Instruments Ltd, Burry St Edmunds, UK). ...

Copper (II) Oxide Nanoparticles Induced Nephrotoxicity In Vitro Conditions
M Abudayyak, EE Guzel, G Özhan - Applied In Vitro Toxicology
.... respectively. The number of DNA breaks was scored under a fluorescent microscope (Olympus BX53; Olympus) at 400 × magnification using an automated image analysis system (Comet Assay IV; Perceptive Instruments). DNA ...

Cytotoxic, genotoxic and biochemical markers of insecticide toxicity evaluated in human peripheral blood lymphocytes and an HepG2 cell line
D Želježic, M Mladinic, S Žunec, AL Vrdoljak, V Kašuba… - Food and Chemical Toxicology
... Scoring and analysis of slides was performed using the Comet Assay IV analysis system (Perceptive Instruments Ltd., UK), connected with an epifluorescence microscope (Olympus BX51, Tokyo, Japan). A total of 100 comets ...

Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama)
J Kostic, S Kolarevic, M Kracun-Kolarevic, M Aborgiba… - Environmental Science and Pollution Research
... A total of 25 cells from both gel replicas of the slide (50 cells per specimen/ 250 cells for each tissue) were randomly scored and analyzed using Comet IV computer software (Perceptive Instruments, UK). Considering this is the first application of comet assay on the freshwater ...

Characterization of urban aerosol: seasonal variation of mutagenicity and genotoxicity of PM2.5, PM1 and semi-volatile organic compounds
C Bocchi, C Bazzini, F Fontana, G Pinto, A Martino… - Mutation Research/Genetic Toxicology
... Duplicate slides were prepared for each dose and, in each slide, 100 randomly selected nuclei (200 cells per dose) were acquired and analysed with specific image analyser software (Comet Assay IV; Perspective Instruments, Suffolk, UK). ...

Chrysobalanus icaco L. fruits inhibit NADPH oxidase complex and protect DNA against doxorubicin-induced damage in Wistar male rats
VP Venancio, MC Marques, MR Almeida, LRB Mariutti… - Journal of Toxicology and Environmental Health
... Immediately before analysis, slides were stained with GelRed (1:10,000 v/v). Tail intensity (percentage of DNA in tail) and tail moment were analyzed in 100 nucleoids per animal using Comet Assay IV software (Perceptive Instruments, Suffolk, UK) and a fluorescence ...

Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay
B Deutschmann, S Kolarevic, W Brack, S Kaisarevic… - Science of The Total Environment
... The alkaline comet assay procedure used was basically as described by Singh et al ... Images were analyzed using Comet IV Computer Software (Perceptive Instruments, UK) and among the parameters available for analyses TI% was chosen as relevant measure of DNA damage ...

PPARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2
GJ Grundy, LM Polo, Z Zeng, SL Rulten, NC Hoch… - Nature Communications
Finally, DNA was stained with Sybr Green I (1:10,000 in PBS) for 30?min. Average tail moments from 50 cells/sample were measured using Comet Assay IV software (Perceptive Instruments, UK).

Dietary zinc deficiency predisposes mice to the development of preneoplastic lesions in chemically-induced hepatocarcinogenesis
GR Romualdo, RL Goto, AAH Fernandes, B Cogliati… - Food and Chemical Toxicology
... Genotoxicity analysis was performed in Comet Assay IV (Perceptive Instruments, UK). Tail intensity (percentage of DNA in the comet tail) was used as a reliable parameter to evaluate DNA damage in 50 nucleoids per slide/two slides per animal

Differential genotoxicity and cytotoxicity of phomoxanthone A isolated from the fungus Phomopsis longicolla in HL60 cells and peripheral blood lymphocytes
GB Pavão, VP Venâncio, ALL de Oliveira… - Toxicology in Vitro, 2016
... High-resolution mass spectra (HRESIMS) were obtained on an UltrO-TOF Bruker-Daltonics instrument (Billarica, USA) equipped with an ... of mass relative to the center of the head of the comet) were evaluated by Comet Assay IV software (Perceptive Instruments, Suffolk, UK ...

The influence of dissolved organic carbon and ultraviolet radiation on the genomic integrity of Daphnia magna
R Wolf, T Andersen, DO Hessen, K Hylland - Functional Ecology
... Eclipse LV 100ND microscope with a Nikon Plan Fluor 20×/0.50 objective (Nikon Corporation, Tokyo, Japan) using Comet Assay IV software (version 4.3; Perceptive Instruments,Bury St Edmund, England, UK). DNA damage was defined as percentage tail ...

DNA Damage Assessment in Zebrafish Embryos Exposed to Monceren® 250 SC Fungicide Using the Alkaline Comet Assay
M Ku-Centurión, B González-Marín… - Zebrafish
... fluorescence microscope equipped with an excitation filter (515–560 nm) and a barrier filter (590 nm). To visualize DNA damage, slides were observed at 40 × magnification using Comet IV Software (Perceptive Instruments). ...

Enhanced susceptibility of obese mice to glycidamide-induced sperm chromatin damage without increased oxidative stress
KB Gutzkow, N Duale, T Danielsen, H Stedingk… - Andrology
... Prominence LC 20 system (Shimadzu Corp., Kyoto, Japan) interfaced to an API 3200 Q-trap instrument with a ... Co., Ltd.; camera: BASLER, A312f-VIS; Vision Technologies, Germany) and a computerised image analysis system (Comet assay IV; Perceptive Instruments, Bury St ...

Chronic Acrylamide Exposure in Male Mice Results in Elevated DNA Damage in the Germline and Heritable Induction of CYP2E1 in the Testes
AL Katen, CG Chambers, B Nixon, SD Roman - Biology of Reproduction
... The DNA integrity of 50 cells per slide was analyzed using Comet Assay IV software (Perceptive Instruments, Suffolk, UK). The relative fluorescence intensity in the comet “tail” versus total intensity of the comet (Tail DNA %) was used as a measure of DNA damage. ...

Genotoxicity assessment of the flavouring agent, perillaldehyde
CA Hobbs, S Taylor, C Beevers, M Lloyd, R Bowen… - Food and Chemical Toxicology
... Comet scoring was performed using “Comet Assay IV” image analysis system (Perceptive Instruments, Suffolk, UK). Measurements of tail intensity (% DNA in tail) and Olive tail moment were generally obtained from at least 150 cells/animal scored on 2–3 slides. ...

Gamma radiation at a human relevant low dose rate is genotoxic in mice
CA Graupner, DM Eide, C Instanes, JM Andersen… - Scientific Reports, 2016
...The raw data of the SCGE was processed using the Comet Assay Spreadsheet Generator Version 1.3.1 (Perceptive Instruments Ltd). The % TI of 50 comets were summarised as median (per gel) ...

Genotoxic effects of cadmium and influence on fitness components of Lymantria dispar caterpillars
D Matic, M Vlahovic, S Kolarevic, VP Mataruga, L Ilijin… - Environmental Pollution, 2016
... in Martinovic et al. (2015). For each group eight slides were prepared and 50 nuclei were analyzed per slide (400 nuclei per group) using Comet IV Computer Software (Perceptive Instruments, UK). The parameter TI% (tail intensity ...

DNA damage response curtails detrimental replication stress and chromosomal instability induced by the dietary carcinogen PhIP
M Mimmler, S Peter, A Kraus, S Stroh, T Nikolova… - Nucleic Acids Research, 2016
... Both alkaline and neutral Comet assays were conducted as reported (36). Comets were analyzed by fluorescence microscopy using a Nikon Microphot-FXA microscope (Perceptive Instruments Ltd., Bury St Edmunds, UK). Fifty cells were scored in each experiment using Comet IV software (Perceptive Instruments Ltd., Bury St Edmunds, UK)...

Oxidative Stress, DNA Damage and DNA Repair in Female Patients with Diabetes Mellitus Type 2
A Grindel, B Guggenberger, L Eichberger… - PloS one, 2016
... DNA damage was expressed in % Tail DNA which was quantified using a fluorescence microscope (Nikon) and the imaging software Comet Assay IV (Perceptive Instruments Ltd). For each sample 100 cells were scored (50 per duplicate) and means were calculated. ...

Genotoxicity, mutagenicity and cytotoxicity of carotenoids extracted from ionic liquid in multiples organs of Wistar rats
PM Larangeira, VV de Rosso, VHP da Silva - Experimental and Toxicologic Pathology, 2016
... The microscope was connected through a black and white camera to an image analysis system (Comet Assay II, Perceptive Instruments, Suffolk, Haverhill, UK) calibrated previously according to the manufacturer's instructions...

Raw and biologically treated paper mill wastewater effluents and the recipient surface waters: Cytotoxic and genotoxic activity and the presence of endocrine disrupting compounds
D Balabanic, M Filipic, AK Klemencic, B Žegura - Science of The Total Environment, 2017
Images of 50 randomly selected nuclei per experimental point were analysed with the image analysis software (Comet Assay IV, Perceptive Instruments, UK).

Occupational exposure to anesthetics leads to genomic instability, cytotoxicity and proliferative changes
KM Souza, LG Braz, FR Nogueira, MB Souza… - … Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2016
... stained with 80 µL SYBR Gold and a total of 100 randomly captured nucleoids per individual (50 from each slide) were blindly analyzed at 400 x magnification using a fluorescent microscope connected to an image analysis system (Comet Assay IV, Perceptive Instruments, UK). ...

Evaluation of Genotoxic Pressure along the Sava River
S Kolarevic, M Aborgiba, M Kracun-Kolarevic, J Kostic… - PloS one, 2016
... The standards for the instrument calibration were prepared on the basis of the multi ... each sample three slides were prepared: one for alkaline and two for Fpg–modified comet assay. ... images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). ...

Magnolia officinalis L. Fortified Gum Improves Resistance of Oral Epithelial Cells Against Inflammation
KJ Walker, JM Imboeck, JM Walker, A Maitra, H Haririan… - The American Journal of Chinese Medicine, 2016
... DNA was stained using 80 µl ethidium bromide solution (50 µg/ml) per slide. DNA damage was detected using COMET assay IV software (Perceptive Instruments, Bury St Edmunds. ...

Genistein and delphinidin antagonize the genotoxic effects of the mycotoxin alternariol in human colon carcinoma cells
G Aichinger, J Beisl, D Marko - Molecular Nutrition & Food Research, 2016
... For image analysis, the “Comet Assay IV” system (Perceptive Instruments, Suffolk, UK) was used to score 50 images per gel pad. In vitro complex of enzyme (ICE) assay The assay was performed as described previously with slight modifications [30]. ...

Acidic horseradish peroxidase activity abolishes genotoxicity of common dyes
BS Janovic, AR Collins, ZM Vujcic, MT Vujcic - Journal of Hazardous Materials, 2016
... Prior to scoring, a drop of water was put on each gel and the slide was covered with a 24 × 60 mm coverslip. Scoring of the comets was carried out using semi-automated image analysis system (Comet Assay IV; Perceptive Instruments). ...

Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay.
S Sipos, J Slobodnik, I Teodorovic, B Vukovic-Gacic… -Science of the Total Environment, 2016
... Images were analyzed using Comet IV Computer Software (Perceptive Instruments, UK) and among the parameters available for analyses TI% was chosen as relevant measure of DNA dam ...

Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride
R Martinovic, S Kolarevic, M Kracun-Kolarevic, J Kostic… - Environmental Toxicology …, 2016
... barrier filter 590 nm). For each specimen 100 nuclei (600 nuclei per group) were scored using Comet IV Computer Software (Perceptive Instruments, UK) and TI% was used as a measure of DNA damage. As the possible indication ...

Combined cyto/genotoxic activity of a selected antineoplastic drug mixture in human circulating blood cells
G Gajski, M Geric, AM Domijan, V Garaj-Vrhovac - Chemosphere, 2016
... The % of tail DNA was used to measure the level of DNA damage and a total of 100 randomly captured nuclei were examined from each slide using software (Comet Assay II; Perceptive Instruments Ltd., Haverhill, Suffolk, UK). ...

Cancer Survivor Study (CASUS) on colorectal patients: longitudinal study on physical activity, fitness, nutrition, and its influences on quality of life, disease recurrence, and survival. Rationale and design
L Soares-Miranda, S Abreu, M Silva, A Peixoto… - International Journal of Colorectal Disease, 2016
... Images will be captured by an online CCD camera and analyzed with the Comet Assay IV analysis software (Perceptive Instruments). For each cell, DNA damage will be expressed as the percentage of DNA in the comet tail. ...

Comet-FISH
G Hovhannisyan, R Aroutiounian - Fluorescence In Situ Hybridization (FISH) Application, 2016
...   Comet Assay IV analysis system (Perceptive Instruments, Suffolk, UK)

Comparison of the DNA damage response in BEAS-2B and A549 cells exposed to titanium dioxide nanoparticles
M Biola-Clier, D Beal, S Caillat, S Libert, L Armand… - Mutagenesis, 2016
... Lysis was performed overnight at 4°C, and electrophoresis was carried out at 25 V (300 mA) for 30 min. Comets were stained using ethidium bromide and measured using comet assay IV (Perceptive Instrument, Bury St Edmunds, UK). ...

Copper (II) oxide nanoparticles induce high toxicity in human neuronal cell
GEE Mahmoud, A Ozhan Gül - Global Journal of Medical Research, 2016
... The number of DNA breaks was scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400X magnification using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

In Vivo Induction of Primary DNA Lesions upon Subchronic Oral Exposure to Multi-walled Carbon Nanotubes
G Gerencsér, T Varjas, K Szendi, C Varga - In Vivo, 2016
...The analyses were performed under fluorescence microscope at ×400 magnification. A total of 50 images per slide were captured and analysed by specific image analysis software (Comet assay IV; Perceptive Instruments Ltd., Bury St Edmunds, UK). ...

In vitro genotoxicity testing of four reference metal nanomaterials, titanium dioxide, zinc oxide, cerium oxide and silver: towards reliable hazard assessment
N El Yamani, AR Collins, E Rundén-Pran, LM Fjellsbø… - Mutagenesis, 2016
... 7.5–8), covered with a cover slip and examined under a fluorescence microscope (Leica DMI 6000 B). Images of comets were scored using Comet Assay IV software (Perceptive Instruments), calculating median % DNA in tail from 50 comets per gel as a measure of DNA SBs. ...

Coffee and oxidative stress: a human intervention study
S Shaposhnikov, T Hatzold, N El Yamani, PM Stavro… - European Journal of Nutrition, 2016
... a cold room. After neutralisation with PBS, and staining with SYBRGold (Invitrogen), slides were scored (50 nucleoids per gel) using the Comet Assay IV image analysis programme (Perceptive Instruments). To increase the ...

Investigation of the toxicity of bismuth oxide nanoparticles in various cell lines
M Abudayyak, E Öztas, M Arici, G Özhan - Chemosphere, 2017
... The number of DNA breaks were scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400× magnification by using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Weak silica nanomaterial-induced genotoxicity can be explained by indirect DNA damage as shown by the OGG1-modified comet assay and genomic analysis
S Pfuhler, TR Downs, AJ Allemang, Y Shan, ME Crosby - Mutagenesis, 2016
... The scoring and evaluation of comet images was carried out using an automated image analysis system (Comet Assay IV™, version 4.11, Perceptive Instruments, Suffolk, UK) with a high-definition CCD video camera (Marlin F-046B, Allied Technologies, Stadtroda, Germany) to ...

Nickel Oxide Nanoparticles Induce Oxidative DNA Damage and Apoptosis in Kidney Cell Line (NRK-52E)
M Abudayyak, E Guzel, G Özhan - Biological Trace Element Research, 2016
... [18] from a pathology perspective. ... medium was de- termined by dynamic light scattering (DLS)(ZetaSizer Nano- ZS, Malvern Instruments, Malvern, UK), For ... Olympus, Tokyo, Japan) at ×400 mag- nification using an automated image analysis system (Perceptive software, Kansas ...

Cellular Differentiation Exacerbates Radiation Sensitivity In Vitro In A Human Dopaminergic Neuronal Model
M TEMELIE, C MUSTACIOSU, ML FLONTA, D SAVU, 2016
... [24] with slight modifications. Slides were analyzed using an image analysis system (Comet Assay IV; Perceptive Instruments, UK) attached to a fluorescence microscope (BX51, Olympus, Japan) and a Marlin F-046 CC camera. ...

Genetic and rat toxicity studies of cyclodextrin glucanotransferase
RR Maronpot, CA Hobbs, J Davis, C Swartz, M Boyle… Toxicology Reports, 2016
... After staining slides with SYBR GoldTM (Molecular Probes, Invitrogen, Carlsbad, CA), 100 cells were scored per sam- ple at 200x magnification without knowledge of sample identity using Comet Assay IV Imaging Software¸ Version 4.3.1 (Perceptive Instruments, Ltd., Suffolk, UK ...

In vitro toxicological assessment of an organosulfur compound from Allium extract: Cytotoxicity, mutagenicity and genotoxicity studies
P Mellado-García, S Maisanaba, M Puerto, AI Prieto… - Food and Chemical Toxicology 2016
... A fluorescence microscope (Olympus BX61) with comet assay IV software (Perceptive Instruments, UK) was used to score at least 100 cells per sample, and the results were expressed as mean % DNA in tail. The percentage ...

Impact of Oligonucleotide Structure, Chemistry, and Delivery Method on In Vitro Cytotoxicity
MM Janas, Y Jiang, MK Schlegel, S Waldron… - Nucleic Acid Therapeutics, 2016
)... Approximately 1,000 cells were plated per comet slide. DNA was stained using SYBR Gold (Thermo Fisher Scientific), imaged using a fluorescence microscope (Zeiss AxioImager.Z1), and scored using the Comet Assay IV Lite software (Perceptive Instruments). ...

Overlapping roles for PARP1 and PARP2 in the recruitment of endogenous XRCC1 and PNKP into oxidized chromatin
H Hanzlikova, W Gittens, K Krejcikova, Z Zeng… - Nucleic Acids Research, 2016
... Tris-HCl (1 M) containing SYBR-G (1:10000) and Antifade (40 µg/ml), and imaged (Nikon Eclipse 50i). Average tail moments from 100 cells per sample were obtained using Comet Assay IV software (Perceptive Instruments). ...

Determination of DNA damage and telomerase activity in stanozolol-treated rats
M Kara, E Ozcagli, P Fragkiadaki, T Kotil… - Experimental and Therapeutic Medicine, 2016
 ... The results were evaluated using the Comet Assay IV image analysis system (Comet Assay IV; Perceptive Instruments Ltd., Suffolk, UK) in order to score DNA damage. Tail intensity data was selected for the evaluation of DNA damage. Telomerase activity assay. ...

An alternative approach to studying the effects of ZnO nanoparticles in cultured human lymphocytes: combining electrochemistry and genotoxicity tests
G Branica, M Mladinic, D Omanovic, D Želježic - Arhiv za higijenu rada i toksikologiju, 2016
... Scoring included 100 comets per each duplicate slide using the Comet Assay IV image analysis system (Perceptive Instruments, Suffolk, UK) coupled with epifluorescence microscope (Olympus BX 51) under 200× magnification. ...

Titanium Dioxide Nanoparticles Evoke Proinflammatory Response during Murine Norovirus Infection Despite Having Minimal Effects on Virus Replication
MP Azevedo - Int J Nano Med & Eng, 2016
... Three slides were scored from each treatment; at least 100 representative cells were selected randomly from each slide and scored via a system comprised of a Nikon 501 fluorescence microscope and Comet IV digital imaging software (Perceptive Instruments, Wiltshire, UK). ...

Antiproliferative and Genotoxic Activities in L929 and HeLa Cell Lines, Mutagenic Effects in Salmonella Strains of Novel Benzoxazole Derivatives
E OKSUZOGLU, E FOTO, F ZILIFDAR… - Latin American Journal of …, 2016
... In this research, these compounds were investigated with the perspective of developing new anti- cancer drugs ... magnification using a fluorescence microscope connected to a CCD camera and an image analysis system (Comet assay IV version 4.11, Perceptive instruments). ...

Moving into advanced nanomaterials. Toxicity of rutile TiO 2 nanoparticles immobilized in nanokaolin nanocomposites on HepG2 cell line
MJ Bessa, C Costa, J Reinosa, C Pereira, S Fraga… - Toxicology and Applied …, 2016
... Slides were analysed using the Comet Assay IV Software (Perceptive Instruments). At least 100 cells were scored (50 for each replicate gel). The percentage of DNA in the comet tail (% tDNA) was used as DNA damage parameter. 2.9. Statistical analysis. ...

Assessment of the genotoxicity of the tyrosine kinase inhibitor imatinib mesylate in cultured fish and human cells
M Novak, B Žegura, J Nunic, G Gajski, M Geric… - Mutation Research/Genetic …, 2016
... the treatment conditions. In each experiment, images of 50 randomly selected nuclei per experimental point were analysed with the image analysis software (Comet Assay IV; Perceptive Instruments Ltd., Haverhill, Suffolk, UK). ...

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