Papers from 2017

Genotoxicity and gene expression analyses of liver and lung tissues of mice treated with titanium dioxide nanoparticles
Y Li, J Yan, W Ding, Y Chen, LM Pack, T Chen - Mutagenesis, 2017
... random from each slide. Scoring was performed on a platform consisting of a Nikon 50i fluorescent microscope (Nikon, USA) and Comet IV digital imaging software (Perceptive Instruments, Wiltshire, UK). Percent (%) DNA in ...

EGCG Prevents High Fat Diet-Induced Changes in Gut Microbiota, Decreases of DNA Strand Breaks, and Changes in Expression and DNA Methylation of Dnmt1 and …
M Remely, F Ferk, S Sterneder, T Setayesh, S Roth… - Oxidative Medicine and …, 2017
... Slides were examined under a fluorescence microscope (Nikon EFD-3, Japan) using 25-fold magnification. DNA migration was determined with a computer aided comet assay image analysis system (Comet Assay IV, Perceptive Instruments, UK). 2.2. Gene Expression Analysis. ...

Cytotoxic, genotoxic and mutagenic evaluation of surface waters from a coal exploration region
CS Porta, DL dos Santos, HV Bernardes… - Chemosphere, 2017
... The stained nucleoids were immediately evaluated at 400× magnification under an Olympus (BX41) fluorescence microscope (excitation filter 515–560 nm; barrier filter 590 nm), equipped with an image analysis system (Comet Assay IV, Perceptive Instruments, UK). ...

Study of TiO2 P25 nanoparticles genotoxicity on lung, blood and liver cells in lung overload and non-overload conditions after repeated respiratory exposure in rats
C Relier, M Dubreuil, OL Garcia, E Cordelli, J Mejia… - Toxicological Sciences, 2017
... as described previously (Trouiller et al., 2009), except lysis occurred overnight. The percentage of DNA in tail was analyzed with Comet Assay IVTM software (Perceptive Instruments) using fluorescent microscope. The median of 150 tail DNA percentages was ...

Loss of CUL4A expression is underlying cisplatin hypersensitivity in colorectal carcinoma cells with acquired trabectedin resistance
B Englinger, M Mair, W Miklos, C Pirker, T Mohr… - British Journal of Cancer, 2017
... DNA migration from nuclei of cells treated with increasing concentrations of cisplatin was studied using the modified alkaline comet assay (Tice et ... of DNA in the tails was measured by use of a computer aided image analysis system (Comet IV, Perceptive Instruments Ltd., Burry ...

Exposure to high levels of fumarate and succinate leads to apoptotic cytotoxicity and altered global DNA methylation profiles in vitro
JF Wentzel, A Lewies, AJ Bronkhorst, E van Dyk… - Biochimie, 2017
... The comet assay (single cell gel electrophoresis) was performed under alkaline conditions as described previously [17 ... sample a minimum of 100 comets were scored and the percentage tail DNA was determined with the Comet IV computer software (Perceptive Instruments Ltd ...

Reference control data obtained from an in vivo comet-micronucleus combination assay using Sprague Dawley rats
S Kasamoto, S Masumori, J Tanaka, M Ueda… - … and Toxicologic Pathology, 2017
... The images were captured by a CCD camera (Allied Vision Technologies GmbH, Stadtroda, Germany) attached to the microscope and analyzed using an image-analysis system (Comet Assay IV system, Perceptive Instruments Ltd, Suffolk, UK). ...

Death of Monocytes through Oxidative Burst of Macrophages and Neutrophils: Killing in Trans
V Ponath, B Kaina - PLOS ONE, 2017
... Germany). At least 100 cells were scored in each experiment by means of Comet IV software (Perceptive Instruments Ltd., Bury St Edmunds, UK). Immunofluorescence of 8-oxo-guanine, XRCC1, pATM, 53BP1 and poly(ADP-ribose). ...

Investigation of the toxicity of bismuth oxide nanoparticles in various cell lines
M Abudayyak, E Öztas, M Arici, G Özhan - Chemosphere, 2017
... The number of DNA breaks were scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400× magnification by using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Nickel oxide nanoparticles are highly toxic to SH-SY5Y neuronal cells
VA Mahmoud, G Elif, Ö Gül - Neurochemistry International, 2017
... The number of DNA breaks was scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400 magnification using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

The influence of dissolved organic carbon and ultraviolet radiation on the genomic integrity of Daphnia magna
R Wolf, T Andersen, DO Hessen, K Hylland - 2016
... Comets were scored on a Nikon Eclipse LV100ND microscope with a Nikon Plan Fluor 20 9 /0Á50 objective (Nikon Corporation, Tokyo, Japan) using COMET ASSAY IV software (version 4.3; Perceptive Instruments, Bury St Edmunds, UK). ...

In Vitro Genotoxicity of Carcinogenic Asarone Isomers
S Haupenthal, K Berg, M Gründken, S Vallicotti… - Food and Function, 2017
... specified as DNA signals in the comet head in comparison to the signals in the comet tail and was analyzed by Comet Assay IV System Software (Perceptive Instruments, Suffolk, UK). To ensure an unbiased analysis, the slides were encoded. ...

Carboxylated nanodiamonds can be used as negative reference in in vitro nanogenotoxicity studies
H Moche, V Paget, D Chevalier, E Lorge, N Claude… - Journal of Applied Toxicology, 2017
... with a previously described protocol (Singh et al., 1988; Tice et al., 2000) and the hOGG1-modified comet assay was carried ... nm barrier filter, connected through a gated CCD camera to Comet Image Analysis System software, version 4.0 (Perceptive Instruments Ltd., Haverhill ...

Comparative study of human neuronal and glial cell sensitivity for in vitro neurogenotoxicity testing
B Laffon, N Fernández-Bertólez, E Pásaro… - Food and Chemical Toxicology, 2017
... 2.5. Comet assay. After treatments with the different compounds, the alkaline comet assay was performed following the general protocol proposed by Singh et al. ... Image capture and analysis were performed using the Comet IV Software (Perceptive Instruments). ...

Tumor-associated APE1 variant exhibits reduced complementation efficiency but does not promote cancer cell phenotypes
JL Illuzzi, DR McNeill, P Bastian, B Brenerman… - Environmental and Molecular Mutagenesis, 2017
... fresh 1× TBE at 25 V for 22 min. Cells were imaged and scored after staining in 1× SYBR Gold using Comet Assay IV software (Perceptive Instruments). Mitochondrial Parameters. Protocols were adapted from Fang et al. [2014]. ...

Role of Macronutrients and Micronutrients in DNA Damage: Results From a Food Frequency Questionnaire
C Ladeira, E Carolino, MC Gomes, M Brito - Nutrition and Metabolic Insights, 2017
... of molecular biol- ogy technology in epidemiologic investigations that, starting from an individual health perspective, upgrades to ... the criteria of scoring comets from each gel described by Collins,40 and the Comet Assay IV software from Perceptive Instruments (Bury St Edmunds)...

Cytotoxicities and genotoxicities of cements based on calcium silicate and of dental formocresol
Y Jeong, H Ko, M Kim - Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2017
...The percentage tail damage, moment, and intensity were measured for more than 50 cells in each sample using an image-analyzing program (Comet Assay IV 4.3 Lite; Perceptive Instruments, Bury St Edmunds, UK). At ...

The GH/IGF-1 axis in a critical period early in life determines cellular DNA repair capacity by altering transcriptional regulation of DNA repair-related genes: implications for the developmental origins of cancer
A Podlutsky, MN Valcarcel-Ares, K Yancey… - GeroScience, 2017
... Invitrogen) at 20× magnification. DNA damage was quantified by measuring the tail DNA content (as a per- centage of total DNA) using the Comet Assay-IV software (Perceptive Instruments, Haverhill, Suffolk, UK). ...

Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures
D Manojlovic, MD Dramicanin, V Miletic, D Mitic-Culafic… - Dental Materials, 2017
... nm, barrier filter of 590 nm, at 400 × magnification. We used image analysis software (Comet Assay IV, Version 4.2, 2007, Perceptive Instruments, UK) for analysis of comets. Although the results of Comet assay could be monitored ... /span>

Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B
D Major, RS Derbes, H Wang, AM Roy-Engel - Gene Reports, 2016
... The digital images, acquired with a SensiCam QE digital camera (Cooke Corporation, Romulus, MI), was used to score tail moments in at least 75 random cells using the Comet Assay IV software (Perceptive Instruments, United Kingdom)...

Ankaferd hemostat induces DNA damage, apoptosis and cytotoxic activity by generating reactive oxygen species in melanoma and normal cell lines
A Kocyigit, EM Guler, IC Haznedaroglu, UY Malkan - Int J Clin Exp Med, 2017
... A computerized image analysis system (Comet Assay IV; Perceptive Instruments, UK) was employed. The DNA percent in tail was used as the primary measure of DNA damage according to Hartmann et al. [21]. All experiments were repeated in triplicate. ...

In vitro evaluation of the genotoxicity of poly(anhydride) nanoparticles designed for oral drug delivery
T Iglesias, M Dusinska, N El Yamani, JM Irache… - International Journal of Pharmaceutics, 2017
... Comets were examined by fluorescent microscope (Nikon Eclipse 50 i, Japan) using the image analysis system Comet Assay IV (Perceptive instruments, UK) and the percentage of DNA in tail (% tail DNA) was used as DNA damage indicator. ...

Molecular mechanisms of discrotophos-induced toxicity in HepG2 cells: The role of CSA in oxidative stress
YC Hseu, TW Hsu, HD Lin, CH Chen, SC Chen - Food and Chemical Toxicology, 2017
... a fluorescence microscope (Nickon Eclipse E200). At least 100 randomly selected images were analyzed from each sample using Comet Assay IV software (Perceptive Instruments, UK). The tail moment comet parameter (mean ...

The effect of flash-freezing temperature on stallion sperm DNA structure 
R Serafini, DD Varner, W Bissett, TL Blanchard… - Theriogenology, 2017
... MD, USA). The neutral comet assay was performed as previously described [12]. Measures were acquired using proprietary software (Comet Assay IV Version 4.11; Perceptive Instruments Ltd., Suffolk, UK). One hundred sperm ...

Different DNA damage response of cis and trans isomers of commonly used UV filter after the exposure on adult human liver stem cells and human lymphoblastoid cells
A Sharma, K Bányiová, P Babica, N El Yamani… - Science of The Total Environment, 2017
... The fluorescence microscope (Nikon Eclipse TS-100) with semi-automated image analysis system (Comet Assay IV; Perceptive instruments) was used to evaluate the DNA damage expressed as DNA % in the tail on TK-6 cells. ...

Antiretroviral Therapy Initiation Alters the Redox System of Asymptomatic HIV-Infected Individuals: A Longitudinal Study
KI Tasca, JT Caleffi, CR Correa, M Gatto, FC Tavares… - Oxidative Medicine and Cellular Longevity, 2017
... The slides were stained by Sybr Gold (Invitrogen, USA). Using an immunofluorescence microscope connected to an image analysis system (comet assay IV, Perceptive Instruments, Sufolk, Haverhill, UK), a total of 50 randomly selected nucleoids were counted for each slide. ...

Cytotoxicity and genotoxicity of lipid nanocapsules
G Le Roux, H Moche, A Nieto, JP Benoit, F Nesslany… - Toxicology in Vitro, 2017
... microscope (Leica Microscopy and Scientific Instruments Group, Heerbrugg, Switzerland) equipped with an excitation filter of 515–560 nm and a barrier filter of 590 nm, connected through a gated CCD camera to Comet Assay IV software (Perceptive Instruments Ltd., UK). ...

The impact of iodinated X-ray contrast agents on formation and toxicity of disinfection by-products in drinking water
CH Jeong, EJ Machek, M Shakeri, SE Duirk, TA Ternes… - Journal of Environmental Sciences, 2017
... camera. A computerized image analysis system (Comet IV, Perspective Instruments, Ltd., Suffolk, UK) was employed to determine the SCGE %Tail DNA value of the nuclei as indices of DNA damage (Kumaravel and Jha, 2006). ...

Assessment of Cellular Responses in Kidney Cells Exposed to Cobalt Oxide Nanoparticles
M Abudayyak, TA GURKAYNAK, G ÖZHAN - Marmara Pharmaceutical Journal, 2017
... The number of DNA breaks were scored under a fluorescent microscope (Olympus BX53, Tokyo, Japan) at 400 magnifications by using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Lagos lagoon sediment organic extracts and polycyclic aromatic hydrocarbons induce embryotoxic, teratogenic and genotoxic effects in Danio rerio (zebrafish) embryos
Sogbanmu, T.O., Nagy, E., Phillips, D.H. et al. Environ Sci Pollut Res - 2017
... All slides were examined at ×40 magnification using a fluorescent microscope (Leica Microscope, Microsystems Wetzler GmbH, Germany) and equipped with a CCD camera (Marlin imaging device) and analysed using the Comet IV software (2006) (Perceptive Instruments, UK). ...

Lack of genotoxicity in vivo for food color additive Tartrazine
Sogbanmu, T.O., Nagy, E., Phillips, D.H. et al. Environ Sci Pollut Res - 2017
... solution). 2.6.3. Comet scoring. A total of 150 randomly selected, non-overlapping cells per animal were scored for DNA damage using the fully validated automated scoring system Comet Assay IV from Perceptive Instruments Ltd. ...

Lack of genotoxicity in vivo for food color additive Allura Red AC
M Bastaki, T Farrell, S Bhusari, K Pant, R Kulkarni - Food and Chemical Toxicology, 2017
... solution). 2.6.3. Comet scoring. A total of 150 randomly selected, non-overlapping cells per animal were scored for DNA damage using the fully validated automated scoring system Comet Assay IV from Perceptive Instruments Ltd. ...

Effect of physicochemical character differences on the genotoxic potency of kaolin
T Kato, T Toyooka, Y Ibuki, S Masuda, M Watanabe… - Genes and Environment, 2017
The percentage tail intensity was measured using Comet Assay IV (Perceptive Instruments, Ltd., Haverhill, UK). For evaluation of DNA damage, the slides were randomised and coded so that the treatment group was blinded to the scorer. The frequency of hedgehogs was not included in the data.”

In vitro uptake and toxicity studies of metal nanoparticles and metal oxide nanoparticles in human HT29 cells
T Schneider, M Westermann, M Glei - Archives of Toxicology, 2017
..... The amount of migrated DNA was deter- mined for 60 DNA spots per slide by Comet Assay IV software (Perceptive Instruments, UK). The intensity of fluorescence in the comet tail expressed as “tail intensity” is the evaluation criteria for DNA damage presented in the graphs. ...

A battery of assays as an integrated approach to evaluate fungal and mycotoxin inhibition properties and cytotoxic/genotoxic side-effects for the prioritization in the screening of thiosemicarbazone derivatives
C Zani, F Bisceglie, FM Restivo, D Feretti, M Pioli… - Food and Chemical …, 2017
... It is in this perspective that our research is aimed at the identification of new compounds, based on natural molecules and ... excitation filter BP 515–560 nm, barrier filter LP 580 nm), using an automatic image analysis system (Comet Assay IV – Perceptive Instruments Ltd, UK). ...

The effect of insecticides chlorpyrifos, a-cypermethrin and imidacloprid on primary DNA damage, TP 53 and c-Myc structural integrity by comet-FISH assay
D Zeljezic, B Vinkovic, V Kasuba, N Kopjar, M Milic… - Chemosphere, 2017
...A total of 100 nucleoids per concentration were scored using the Comet Assay IV analysis system (Perceptive Instruments, Suffolk, UK), epifluorescence microscope (Olympus, BX 51, Tokyo, Japan), 20× objective. ...

Betamethasone causes intergenerational reproductive impairment in male rats
C dos Santos Borges, TL Pacheco, KP da Silva… - Reproductive Toxicology, 2017
... until analysis. For microscopy, the slides were stained with SYBR ® Gold (1:10,000; Invitrogen, Waltham, MA) and analyzed using the Comet Assay IV software (Comet Assay IV, Perceptive Instruments, Wiltshire, UK). DNA damage ...

Comparison of the toxicity of sintered and unsintered indium-tin oxide particles in murine macrophage and epidermal cells
NS Olgun, AM Morris, TL Barber, AB Stefaniak… - Toxicology and Applied …, 2017
... solution, cells were then lysed and subjected to electrophoresis at 21 V and 50 mA for 1 h (Trevigen, Inc., Gaithersburg ... The Comet Assay IV software (Perceptive Instruments, Bury Saint Edmonds, United Kingdom) was used to count comet tails ...

Slight hypercalcemia is not associated with positive responses in the Comet-Assay in male rat liver
A Thiel, A Hamel, K Schaefer, R Cardoso, P Beilstein - Mutation Research/Genetic …, 2017
Computerized image analysis of selected cells was performed using a Perceptive Instruments COMET ASSAY IV image analysis system. A total of 150 liver cells per animal (75 cells per slide) were evaluated. The excessively damaged cells (i.e. ‘hedgehog’ or ‘ghost’ comets) were counted but excluded from analysis .. /span>

Cytotoxicity and genotoxicity of anticancer drug residues and their mixtures in experimental model with zebrafish liver cells
M Novak, B Žegura, B Modic, E Heath, M Filipic - Science of The Total Environment, 2017
... with ethidium bromide (10 µg/mL) and images of 50 randomly selected nuclei per experimental point were analysed using a fluorescence microscope (Nikon, Eclipse 800) at 400 × magnification and the image analysis software (Comet Assay IV, Perceptive Instruments Ltd., UK ...

Dynamic In Vivo Profiling of DNA Damage and Repair after Radiotherapy Using Canine Patients as a Model
N Schulz, H Chaachouay, KJ Nytko, MS Weyland… - International Journal of …, 2017
... LMPA, Trevigen, Gaithersburg, MA, USA) in PBS, 37 C. Subsequently, 25 µL of agarose-cell solution was pipetted onto 20-well slides (Trevigen, Gaithersburg, MA, USA ... To provide a quantitative analysis of obtained comets (%DNA in tail, tail intensity), the COMET IV® scoring ...

DNA damage in Wistar Kyoto rats exercised during pregnancy
MS Corrêa, RB Gelaleti, GF Bento, DC Damasceno… - Acta Cirurgica Brasileira, 2017
.One hundred nucleoids per sample (50/slide) were assessed by automated image analysis (Comet Assay IV, Perceptive Instruments, UK). Tail intensity (defined as the percentage of DNA in the tail) was used as a measure of DNA damage.

Vitamin E Modifies High-Fat Diet-Induced Increase of DNA Strand Breaks, and Changes in Expression and DNA Methylation of Dnmt1 and MLH1 in C57BL/6J Male Mice
N Schulz, H Chaachouay, KJ Nytko, MS Weyland… - International Journal of …, 2017
Three slides were prepared per experimental time point and 50 cells were evaluated from each slide. Slides were examined under a fluorescence microscope (Nikon EFD-3, Tokyo, Japan) using 25-fold magnification. DNA migration was determined with a computer-aided comet assay image analysis system (Comet Assay IV, Perceptive Instruments, Bury St Edmunds, UK).

Assessing the impact of Benzo[a]pyrene on Marine Mussels: Application of a novel targeted low density microarray complementing classical biomarker responses
M Banni, S Sforzini, VM Arlt, A Barranger, LJ Dallas… - PLOS ONE, 2017
Comet 4 image analysis system (Perceptive Instruments, Bury St Edmunds, UK). One hundred nucleoids were assessed per slide; all samples were measured blind. Tail intensity (% tail DNA), defined as the percentage of DNA migrated from the head of the comet into the tail, was used as a measure of DNA damage induced.

Ecotoxicology and Genotoxicology: Non-traditional Terrestrial Models, Chapter 3 - Aporrectodea longa (Annelida, Lumbricidae): A suitable Earthworm Model for Genotoxicity Evaluation in the Environment
Kirk T. Semple and Francis L. Martin, Issues in Toxicology, Royal Society of Chemistry 2017
... The slides were then stained with aqueous ethidium bromide (20 ng mlÀ1) and visualised by epifluorescence using a Leitz Dialux 20 EB microscope, with comet tail lengths (CTL; µm) measured using Comet Assay VI software (Perceptive Instruments, UK). ...

Continuous in vitro exposure of intestinal epithelial cells to E171 food additive causes oxidative stress, inducing oxidation of DNA bases but no endoplasmic reticulum stress
M Dorier, D Béal, C Marie-Desvergne, M Dubosson… - Nanotoxicology, 2017
... 7.4, then stained with Gel Red (VWR). %tail DNA was measured on 50 nuclei using Comet IV software (Perceptive instruments). As positive control for comet-Fpg, A549 cells were exposed to 1 µM riboflavin for 20 min at 37 °C and then UVA-irradiated (010.0 J/cm²). As ...

Gold nanorods induce early embryonic developmental delay and lethality in zebrafish (Danio rerio)
B Mesquita, I Lopes, S Silva, MJ Bessa, M Starykevich… - Journal of Toxicology and Environmental Health, Part A, 2017
... scorer performed the comet analysis using a fluorescence microscope (Nikon Eclipse E400 microscope attached to an epifluorescence illuminator Nikon C-SHG1) with 500× magnification and the image analysis software Comet Assay IV (Perceptive Instruments, Suffolk, UK). ...

Dose-rate effect of ultrashort electron beam radiation on DNA damage and repair in vitro
MN Babayan, G Hovhannisyan, B Grigoryan… - Journal of Radiation Research, 2017
... At least 150 cells were examined for each experimental point. Images were analysed using Comet Assay IV software (Perceptive Instruments, UK). Statistical analysis All experiments were repeated at least three times; triplicate cultures were scored for an experimental point. ...

Particulate emissions from modern and old technology wood combustion induce distinct time-dependent patterns of toxicological responses in vitro
SH Mikko, H Maija-Riitta, U Oskari, K Stefanie… - Toxicology in Vitro, 2017
... The comet analysis was performed in ethidium bromide-stained slides analysing 100 cells/nuclei per test sample in each experiment using the comet assay IV (Perceptive Instruments Ltd., UK) image analysing system. Three replicate experiments were analysed. ...

Approaches of Whole Blood Thawing for Genotoxicity Analysis in Rats
AO Netto, RB Gelaleti, RG Serrano - J Toxicol Pharmacol, 2017
... was determined. Tail Intensity, according to Comet Assay IV Image System (Perceptive Instruments, UK), is defined as 'the sum of all intensity values in the tail region minus those which are part of the mirrored head region. The ...

Effects of sub-chronic exposure to terbuthylazine on DNA damage, oxidative stress and parent compound/metabolite levels in adult male rats
BT Lovakovic, A Pizent, V Kašuba, N Kopjar, V Micek… - Food and Chemical Toxicology 2017
... Nucleoids with >80% of DNA in the tail were classified as “hedgehogs”, and were excluded from the analysis. The level of DNA damage in individual cells was evaluated using the Comet Assay IV image analysis system (Perceptive Instruments Ltd., Suffolk, Halstead, UK). ...

Genotoxic evaluation of poly(anhydride) nanoparticles in the gastrointestinal tract of mice
T Iglesias, JM Irache, M Butinar, B Turk, AL de Cerain… - International Journal of Pharmaceutics 2017
... Slides were then examined by a fluorescent microscope (Nikon Eclipse 50 i, Japan) using the image analysis system Comet assay IV (Perceptive instruments), scoring 75 comets per gel (2 gels/slide, 150 comets/sample). Percentage ...

Sodium fluoride adversely affects ovarian development and reproduction in Drosophila melanogaster
BS Khatun, P Rajak, M Dutta, S Roy - Chemosphere, 2017
... microscope at 400X magnification. Images were scored randomly using 'Comet Assay IV' software of Perceptive Instruments. Duplicate sets of slides were prepared and examined for each treatment category. 2.12. Statistical analysis. ...

In situ biomonitoring of air quality in rural and urban environments of Mexico Valley through genotoxicity evaluated in wild plants
S Gómez-Arroyo, J Cortés-Eslava, P Loza-Gómez… - Atmospheric Pollution …, 2017
... Slides were then examined by a fluorescent microscope (Nikon Eclipse 50 i, Japan) using the image analysis system Comet assay IV (Perceptive instruments), scoring 75 comets per gel (2 gels/slide, 150 comets/sample). Percentage ...

Cytotoxicity and genotoxicity of fenoxaprop-p-ethyl and fluzifob-p-butyl herbicides
B Ulus, M Çeliksöz, G Özhan - 2017
... DNA was stained with ethidium bromide (20 mg/mL) just before slide examination under a fluorescent microscope (Olympus, Japan) at 400 magnifi- cation by using an automated image analysis system (Comet Assay IV, Perceptive Instruments, UK). ...

A type I combi-targeting approach for the design of molecules with enhanced potency against BRCA1/2 mutant- and O6-methylguanine-DNA methyltransferase (mgmt)- expressing tumour cells
ZS Mouhri, E Goodfellow, B Jean-Claude - BMC Cancer, 2017
Comets were visualized at 400X magnification and DNA damage was quantified using Comet Assay IV software to calculate tail moments...

In Vitro Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell
ZS Mouhri, E Goodfellow, B Jean-Claude - BMC Cancer, 2017
... The number of DNA breaks were scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400 magnification using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Integrated assessment of toxic effects of maghemite (?-Fe2O3) nanoparticles in zebrafishs
RAR Villacis, S José Filho, B Piña, RB Azevedo… - Aquatic Toxicology, 2017
... bromide (20 µg/mL) and analyzed using a fluorescence microscope (ZEISS Axioskop 2-HAL100, at 400× magnification), fitted with a 510–560 nm filter and a 590 nm blocking filter; and the Comet IV Lite v4.3 system image analysis software (Perceptive Instruments, Suffolk, UK). ...

About Us

This site is maintained by Perceptive Instruments, industry-leading experts in image analysis for the comet assay.

We regularly attend comet assay workshops and contribute to the debate towards regulatory acceptance of the assay.

About Comet Assay IV

Comet Assay IV is the world's fastest, most consistent comet assay scoring system.

For our full range of products, head over to www.perceptive.co.uk