Papers from 2017

Genotoxicity and gene expression analyses of liver and lung tissues of mice treated with titanium dioxide nanoparticles
Y Li, J Yan, W Ding, Y Chen, LM Pack, T Chen - Mutagenesis, 2017
... random from each slide. Scoring was performed on a platform consisting of a Nikon 50i fluorescent microscope (Nikon, USA) and Comet IV digital imaging software (Perceptive Instruments, Wiltshire, UK). Percent (%) DNA in ...

EGCG Prevents High Fat Diet-Induced Changes in Gut Microbiota, Decreases of DNA Strand Breaks, and Changes in Expression and DNA Methylation of Dnmt1 and …
M Remely, F Ferk, S Sterneder, T Setayesh, S Roth… - Oxidative Medicine and …, 2017
... Slides were examined under a fluorescence microscope (Nikon EFD-3, Japan) using 25-fold magnification. DNA migration was determined with a computer aided comet assay image analysis system (Comet Assay IV, Perceptive Instruments, UK). 2.2. Gene Expression Analysis. ...

Cytotoxic, genotoxic and mutagenic evaluation of surface waters from a coal exploration region
CS Porta, DL dos Santos, HV Bernardes… - Chemosphere, 2017
... The stained nucleoids were immediately evaluated at 400× magnification under an Olympus (BX41) fluorescence microscope (excitation filter 515–560 nm; barrier filter 590 nm), equipped with an image analysis system (Comet Assay IV, Perceptive Instruments, UK). ...

Study of TiO2 P25 nanoparticles genotoxicity on lung, blood and liver cells in lung overload and non-overload conditions after repeated respiratory exposure in rats
C Relier, M Dubreuil, OL Garcia, E Cordelli, J Mejia… - Toxicological Sciences, 2017
... as described previously (Trouiller et al., 2009), except lysis occurred overnight. The percentage of DNA in tail was analyzed with Comet Assay IVTM software (Perceptive Instruments) using fluorescent microscope. The median of 150 tail DNA percentages was ...

Loss of CUL4A expression is underlying cisplatin hypersensitivity in colorectal carcinoma cells with acquired trabectedin resistance
B Englinger, M Mair, W Miklos, C Pirker, T Mohr… - British Journal of Cancer, 2017
... DNA migration from nuclei of cells treated with increasing concentrations of cisplatin was studied using the modified alkaline comet assay (Tice et ... of DNA in the tails was measured by use of a computer aided image analysis system (Comet IV, Perceptive Instruments Ltd., Burry ...

Exposure to high levels of fumarate and succinate leads to apoptotic cytotoxicity and altered global DNA methylation profiles in vitro
JF Wentzel, A Lewies, AJ Bronkhorst, E van Dyk… - Biochimie, 2017
... The comet assay (single cell gel electrophoresis) was performed under alkaline conditions as described previously [17 ... sample a minimum of 100 comets were scored and the percentage tail DNA was determined with the Comet IV computer software (Perceptive Instruments Ltd ...

Reference control data obtained from an in vivo comet-micronucleus combination assay using Sprague Dawley rats
S Kasamoto, S Masumori, J Tanaka, M Ueda… - … and Toxicologic Pathology, 2017
... The images were captured by a CCD camera (Allied Vision Technologies GmbH, Stadtroda, Germany) attached to the microscope and analyzed using an image-analysis system (Comet Assay IV system, Perceptive Instruments Ltd, Suffolk, UK). ...

Death of Monocytes through Oxidative Burst of Macrophages and Neutrophils: Killing in Trans
V Ponath, B Kaina - PLOS ONE, 2017
... Germany). At least 100 cells were scored in each experiment by means of Comet IV software (Perceptive Instruments Ltd., Bury St Edmunds, UK). Immunofluorescence of 8-oxo-guanine, XRCC1, pATM, 53BP1 and poly(ADP-ribose). ...

Investigation of the toxicity of bismuth oxide nanoparticles in various cell lines
M Abudayyak, E Öztas, M Arici, G Özhan - Chemosphere, 2017
... The number of DNA breaks were scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400× magnification by using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Nickel oxide nanoparticles are highly toxic to SH-SY5Y neuronal cells
VA Mahmoud, G Elif, Ö Gül - Neurochemistry International, 2017
... The number of DNA breaks was scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400 magnification using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

The influence of dissolved organic carbon and ultraviolet radiation on the genomic integrity of Daphnia magna
R Wolf, T Andersen, DO Hessen, K Hylland - 2016
... Comets were scored on a Nikon Eclipse LV100ND microscope with a Nikon Plan Fluor 20 9 /0Á50 objective (Nikon Corporation, Tokyo, Japan) using COMET ASSAY IV software (version 4.3; Perceptive Instruments, Bury St Edmunds, UK). ...

In Vitro Genotoxicity of Carcinogenic Asarone Isomers
S Haupenthal, K Berg, M Gründken, S Vallicotti… - Food and Function, 2017
... specified as DNA signals in the comet head in comparison to the signals in the comet tail and was analyzed by Comet Assay IV System Software (Perceptive Instruments, Suffolk, UK). To ensure an unbiased analysis, the slides were encoded. ...

Carboxylated nanodiamonds can be used as negative reference in in vitro nanogenotoxicity studies
H Moche, V Paget, D Chevalier, E Lorge, N Claude… - Journal of Applied Toxicology, 2017
... with a previously described protocol (Singh et al., 1988; Tice et al., 2000) and the hOGG1-modified comet assay was carried ... nm barrier filter, connected through a gated CCD camera to Comet Image Analysis System software, version 4.0 (Perceptive Instruments Ltd., Haverhill ...

Comparative study of human neuronal and glial cell sensitivity for in vitro neurogenotoxicity testing
B Laffon, N Fernández-Bertólez, E Pásaro… - Food and Chemical Toxicology, 2017
... 2.5. Comet assay. After treatments with the different compounds, the alkaline comet assay was performed following the general protocol proposed by Singh et al. ... Image capture and analysis were performed using the Comet IV Software (Perceptive Instruments). ...

Tumor-associated APE1 variant exhibits reduced complementation efficiency but does not promote cancer cell phenotypes
JL Illuzzi, DR McNeill, P Bastian, B Brenerman… - Environmental and Molecular Mutagenesis, 2017
... fresh 1× TBE at 25 V for 22 min. Cells were imaged and scored after staining in 1× SYBR Gold using Comet Assay IV software (Perceptive Instruments). Mitochondrial Parameters. Protocols were adapted from Fang et al. [2014]. ...

Role of Macronutrients and Micronutrients in DNA Damage: Results From a Food Frequency Questionnaire
C Ladeira, E Carolino, MC Gomes, M Brito - Nutrition and Metabolic Insights, 2017
... of molecular biol- ogy technology in epidemiologic investigations that, starting from an individual health perspective, upgrades to ... the criteria of scoring comets from each gel described by Collins,40 and the Comet Assay IV software from Perceptive Instruments (Bury St Edmunds)...

Cytotoxicities and genotoxicities of cements based on calcium silicate and of dental formocresol
Y Jeong, H Ko, M Kim - Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2017
...The percentage tail damage, moment, and intensity were measured for more than 50 cells in each sample using an image-analyzing program (Comet Assay IV 4.3 Lite; Perceptive Instruments, Bury St Edmunds, UK). At ...

The GH/IGF-1 axis in a critical period early in life determines cellular DNA repair capacity by altering transcriptional regulation of DNA repair-related genes: implications for the developmental origins of cancer
A Podlutsky, MN Valcarcel-Ares, K Yancey… - GeroScience, 2017
... Invitrogen) at 20× magnification. DNA damage was quantified by measuring the tail DNA content (as a per- centage of total DNA) using the Comet Assay-IV software (Perceptive Instruments, Haverhill, Suffolk, UK). ...

Cytotoxicity and genotoxicity of a low-shrinkage monomer and monoacylphosphine oxide photoinitiator: Comparative analyses of individual toxicity and combination effects in mixtures
D Manojlovic, MD Dramicanin, V Miletic, D Mitic-Culafic… - Dental Materials, 2017
... nm, barrier filter of 590 nm, at 400 × magnification. We used image analysis software (Comet Assay IV, Version 4.2, 2007, Perceptive Instruments, UK) for analysis of comets. Although the results of Comet assay could be monitored ... /span>

Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B
D Major, RS Derbes, H Wang, AM Roy-Engel - Gene Reports, 2016
... The digital images, acquired with a SensiCam QE digital camera (Cooke Corporation, Romulus, MI), was used to score tail moments in at least 75 random cells using the Comet Assay IV software (Perceptive Instruments, United Kingdom)...

Ankaferd hemostat induces DNA damage, apoptosis and cytotoxic activity by generating reactive oxygen species in melanoma and normal cell lines
A Kocyigit, EM Guler, IC Haznedaroglu, UY Malkan - Int J Clin Exp Med, 2017
... A computerized image analysis system (Comet Assay IV; Perceptive Instruments, UK) was employed. The DNA percent in tail was used as the primary measure of DNA damage according to Hartmann et al. [21]. All experiments were repeated in triplicate. ...

In vitro evaluation of the genotoxicity of poly(anhydride) nanoparticles designed for oral drug delivery
T Iglesias, M Dusinska, N El Yamani, JM Irache… - International Journal of Pharmaceutics, 2017
... Comets were examined by fluorescent microscope (Nikon Eclipse 50 i, Japan) using the image analysis system Comet Assay IV (Perceptive instruments, UK) and the percentage of DNA in tail (% tail DNA) was used as DNA damage indicator. ...

Molecular mechanisms of discrotophos-induced toxicity in HepG2 cells: The role of CSA in oxidative stress
YC Hseu, TW Hsu, HD Lin, CH Chen, SC Chen - Food and Chemical Toxicology, 2017
... a fluorescence microscope (Nickon Eclipse E200). At least 100 randomly selected images were analyzed from each sample using Comet Assay IV software (Perceptive Instruments, UK). The tail moment comet parameter (mean ...

The effect of flash-freezing temperature on stallion sperm DNA structure 
R Serafini, DD Varner, W Bissett, TL Blanchard… - Theriogenology, 2017
... MD, USA). The neutral comet assay was performed as previously described [12]. Measures were acquired using proprietary software (Comet Assay IV Version 4.11; Perceptive Instruments Ltd., Suffolk, UK). One hundred sperm ...

Different DNA damage response of cis and trans isomers of commonly used UV filter after the exposure on adult human liver stem cells and human lymphoblastoid cells
A Sharma, K Bányiová, P Babica, N El Yamani… - Science of The Total Environment, 2017
... The fluorescence microscope (Nikon Eclipse TS-100) with semi-automated image analysis system (Comet Assay IV; Perceptive instruments) was used to evaluate the DNA damage expressed as DNA % in the tail on TK-6 cells. ...

Antiretroviral Therapy Initiation Alters the Redox System of Asymptomatic HIV-Infected Individuals: A Longitudinal Study
KI Tasca, JT Caleffi, CR Correa, M Gatto, FC Tavares… - Oxidative Medicine and Cellular Longevity, 2017
... The slides were stained by Sybr Gold (Invitrogen, USA). Using an immunofluorescence microscope connected to an image analysis system (comet assay IV, Perceptive Instruments, Sufolk, Haverhill, UK), a total of 50 randomly selected nucleoids were counted for each slide. ...

Cytotoxicity and genotoxicity of lipid nanocapsules
G Le Roux, H Moche, A Nieto, JP Benoit, F Nesslany… - Toxicology in Vitro, 2017
... microscope (Leica Microscopy and Scientific Instruments Group, Heerbrugg, Switzerland) equipped with an excitation filter of 515–560 nm and a barrier filter of 590 nm, connected through a gated CCD camera to Comet Assay IV software (Perceptive Instruments Ltd., UK). ...

The impact of iodinated X-ray contrast agents on formation and toxicity of disinfection by-products in drinking water
CH Jeong, EJ Machek, M Shakeri, SE Duirk, TA Ternes… - Journal of Environmental Sciences, 2017
... camera. A computerized image analysis system (Comet IV, Perspective Instruments, Ltd., Suffolk, UK) was employed to determine the SCGE %Tail DNA value of the nuclei as indices of DNA damage (Kumaravel and Jha, 2006). ...

Assessment of Cellular Responses in Kidney Cells Exposed to Cobalt Oxide Nanoparticles
M Abudayyak, TA GURKAYNAK, G ÖZHAN - Marmara Pharmaceutical Journal, 2017
... The number of DNA breaks were scored under a fluorescent microscope (Olympus BX53, Tokyo, Japan) at 400 magnifications by using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Lagos lagoon sediment organic extracts and polycyclic aromatic hydrocarbons induce embryotoxic, teratogenic and genotoxic effects in Danio rerio (zebrafish) embryos
Sogbanmu, T.O., Nagy, E., Phillips, D.H. et al. Environ Sci Pollut Res - 2017
... All slides were examined at ×40 magnification using a fluorescent microscope (Leica Microscope, Microsystems Wetzler GmbH, Germany) and equipped with a CCD camera (Marlin imaging device) and analysed using the Comet IV software (2006) (Perceptive Instruments, UK). ...

Lack of genotoxicity in vivo for food color additive Tartrazine
Sogbanmu, T.O., Nagy, E., Phillips, D.H. et al. Environ Sci Pollut Res - 2017
... solution). 2.6.3. Comet scoring. A total of 150 randomly selected, non-overlapping cells per animal were scored for DNA damage using the fully validated automated scoring system Comet Assay IV from Perceptive Instruments Ltd. ...

Lack of genotoxicity in vivo for food color additive Allura Red AC
M Bastaki, T Farrell, S Bhusari, K Pant, R Kulkarni - Food and Chemical Toxicology, 2017
... solution). 2.6.3. Comet scoring. A total of 150 randomly selected, non-overlapping cells per animal were scored for DNA damage using the fully validated automated scoring system Comet Assay IV from Perceptive Instruments Ltd. ...

Effect of physicochemical character differences on the genotoxic potency of kaolin
T Kato, T Toyooka, Y Ibuki, S Masuda, M Watanabe… - Genes and Environment, 2017
The percentage tail intensity was measured using Comet Assay IV (Perceptive Instruments, Ltd., Haverhill, UK). For evaluation of DNA damage, the slides were randomised and coded so that the treatment group was blinded to the scorer. The frequency of hedgehogs was not included in the data.”

In vitro uptake and toxicity studies of metal nanoparticles and metal oxide nanoparticles in human HT29 cells
T Schneider, M Westermann, M Glei - Archives of Toxicology, 2017
..... The amount of migrated DNA was deter- mined for 60 DNA spots per slide by Comet Assay IV software (Perceptive Instruments, UK). The intensity of fluorescence in the comet tail expressed as “tail intensity” is the evaluation criteria for DNA damage presented in the graphs. ...

A battery of assays as an integrated approach to evaluate fungal and mycotoxin inhibition properties and cytotoxic/genotoxic side-effects for the prioritization in the screening of thiosemicarbazone derivatives
C Zani, F Bisceglie, FM Restivo, D Feretti, M Pioli… - Food and Chemical …, 2017
... It is in this perspective that our research is aimed at the identification of new compounds, based on natural molecules and ... excitation filter BP 515–560 nm, barrier filter LP 580 nm), using an automatic image analysis system (Comet Assay IV – Perceptive Instruments Ltd, UK). ...

The effect of insecticides chlorpyrifos, a-cypermethrin and imidacloprid on primary DNA damage, TP 53 and c-Myc structural integrity by comet-FISH assay
D Zeljezic, B Vinkovic, V Kasuba, N Kopjar, M Milic… - Chemosphere, 2017
...A total of 100 nucleoids per concentration were scored using the Comet Assay IV analysis system (Perceptive Instruments, Suffolk, UK), epifluorescence microscope (Olympus, BX 51, Tokyo, Japan), 20× objective. ...

Betamethasone causes intergenerational reproductive impairment in male rats
C dos Santos Borges, TL Pacheco, KP da Silva… - Reproductive Toxicology, 2017
... until analysis. For microscopy, the slides were stained with SYBR ® Gold (1:10,000; Invitrogen, Waltham, MA) and analyzed using the Comet Assay IV software (Comet Assay IV, Perceptive Instruments, Wiltshire, UK). DNA damage ...

Comparison of the toxicity of sintered and unsintered indium-tin oxide particles in murine macrophage and epidermal cells
NS Olgun, AM Morris, TL Barber, AB Stefaniak… - Toxicology and Applied …, 2017
... solution, cells were then lysed and subjected to electrophoresis at 21 V and 50 mA for 1 h (Trevigen, Inc., Gaithersburg ... The Comet Assay IV software (Perceptive Instruments, Bury Saint Edmonds, United Kingdom) was used to count comet tails ...

Slight hypercalcemia is not associated with positive responses in the Comet-Assay in male rat liver
A Thiel, A Hamel, K Schaefer, R Cardoso, P Beilstein - Mutation Research/Genetic …, 2017
Computerized image analysis of selected cells was performed using a Perceptive Instruments COMET ASSAY IV image analysis system. A total of 150 liver cells per animal (75 cells per slide) were evaluated. The excessively damaged cells (i.e. ‘hedgehog’ or ‘ghost’ comets) were counted but excluded from analysis .. /span>

Cytotoxicity and genotoxicity of anticancer drug residues and their mixtures in experimental model with zebrafish liver cells
M Novak, B Žegura, B Modic, E Heath, M Filipic - Science of The Total Environment, 2017
... with ethidium bromide (10 µg/mL) and images of 50 randomly selected nuclei per experimental point were analysed using a fluorescence microscope (Nikon, Eclipse 800) at 400 × magnification and the image analysis software (Comet Assay IV, Perceptive Instruments Ltd., UK ...

Dynamic In Vivo Profiling of DNA Damage and Repair after Radiotherapy Using Canine Patients as a Model
N Schulz, H Chaachouay, KJ Nytko, MS Weyland… - International Journal of …, 2017
... LMPA, Trevigen, Gaithersburg, MA, USA) in PBS, 37 C. Subsequently, 25 µL of agarose-cell solution was pipetted onto 20-well slides (Trevigen, Gaithersburg, MA, USA ... To provide a quantitative analysis of obtained comets (%DNA in tail, tail intensity), the COMET IV® scoring ...

DNA damage in Wistar Kyoto rats exercised during pregnancy
MS Corrêa, RB Gelaleti, GF Bento, DC Damasceno… - Acta Cirurgica Brasileira, 2017
.One hundred nucleoids per sample (50/slide) were assessed by automated image analysis (Comet Assay IV, Perceptive Instruments, UK). Tail intensity (defined as the percentage of DNA in the tail) was used as a measure of DNA damage.

Vitamin E Modifies High-Fat Diet-Induced Increase of DNA Strand Breaks, and Changes in Expression and DNA Methylation of Dnmt1 and MLH1 in C57BL/6J Male Mice
N Schulz, H Chaachouay, KJ Nytko, MS Weyland… - International Journal of …, 2017
Three slides were prepared per experimental time point and 50 cells were evaluated from each slide. Slides were examined under a fluorescence microscope (Nikon EFD-3, Tokyo, Japan) using 25-fold magnification. DNA migration was determined with a computer-aided comet assay image analysis system (Comet Assay IV, Perceptive Instruments, Bury St Edmunds, UK).

Assessing the impact of Benzo[a]pyrene on Marine Mussels: Application of a novel targeted low density microarray complementing classical biomarker responses
M Banni, S Sforzini, VM Arlt, A Barranger, LJ Dallas… - PLOS ONE, 2017
Comet 4 image analysis system (Perceptive Instruments, Bury St Edmunds, UK). One hundred nucleoids were assessed per slide; all samples were measured blind. Tail intensity (% tail DNA), defined as the percentage of DNA migrated from the head of the comet into the tail, was used as a measure of DNA damage induced.

Ecotoxicology and Genotoxicology: Non-traditional Terrestrial Models, Chapter 3 - Aporrectodea longa (Annelida, Lumbricidae): A suitable Earthworm Model for Genotoxicity Evaluation in the Environment
Kirk T. Semple and Francis L. Martin, Issues in Toxicology, Royal Society of Chemistry 2017
... The slides were then stained with aqueous ethidium bromide (20 ng mlÀ1) and visualised by epifluorescence using a Leitz Dialux 20 EB microscope, with comet tail lengths (CTL; µm) measured using Comet Assay VI software (Perceptive Instruments, UK). ...

Continuous in vitro exposure of intestinal epithelial cells to E171 food additive causes oxidative stress, inducing oxidation of DNA bases but no endoplasmic reticulum stress
M Dorier, D Béal, C Marie-Desvergne, M Dubosson… - Nanotoxicology, 2017
... 7.4, then stained with Gel Red (VWR). %tail DNA was measured on 50 nuclei using Comet IV software (Perceptive instruments). As positive control for comet-Fpg, A549 cells were exposed to 1 µM riboflavin for 20 min at 37 °C and then UVA-irradiated (010.0 J/cm²). As ...

Gold nanorods induce early embryonic developmental delay and lethality in zebrafish (Danio rerio)
B Mesquita, I Lopes, S Silva, MJ Bessa, M Starykevich… - Journal of Toxicology and Environmental Health, Part A, 2017
... scorer performed the comet analysis using a fluorescence microscope (Nikon Eclipse E400 microscope attached to an epifluorescence illuminator Nikon C-SHG1) with 500× magnification and the image analysis software Comet Assay IV (Perceptive Instruments, Suffolk, UK). ...

Dose-rate effect of ultrashort electron beam radiation on DNA damage and repair in vitro
MN Babayan, G Hovhannisyan, B Grigoryan… - Journal of Radiation Research, 2017
... At least 150 cells were examined for each experimental point. Images were analysed using Comet Assay IV software (Perceptive Instruments, UK). Statistical analysis All experiments were repeated at least three times; triplicate cultures were scored for an experimental point. ...

Particulate emissions from modern and old technology wood combustion induce distinct time-dependent patterns of toxicological responses in vitro
SH Mikko, H Maija-Riitta, U Oskari, K Stefanie… - Toxicology in Vitro, 2017
... The comet analysis was performed in ethidium bromide-stained slides analysing 100 cells/nuclei per test sample in each experiment using the comet assay IV (Perceptive Instruments Ltd., UK) image analysing system. Three replicate experiments were analysed. ...

Approaches of Whole Blood Thawing for Genotoxicity Analysis in Rats
AO Netto, RB Gelaleti, RG Serrano - J Toxicol Pharmacol, 2017
... was determined. Tail Intensity, according to Comet Assay IV Image System (Perceptive Instruments, UK), is defined as 'the sum of all intensity values in the tail region minus those which are part of the mirrored head region. The ...

Effects of sub-chronic exposure to terbuthylazine on DNA damage, oxidative stress and parent compound/metabolite levels in adult male rats
BT Lovakovic, A Pizent, V Kašuba, N Kopjar, V Micek… - Food and Chemical Toxicology 2017
... Nucleoids with >80% of DNA in the tail were classified as “hedgehogs”, and were excluded from the analysis. The level of DNA damage in individual cells was evaluated using the Comet Assay IV image analysis system (Perceptive Instruments Ltd., Suffolk, Halstead, UK). ...

Genotoxic evaluation of poly(anhydride) nanoparticles in the gastrointestinal tract of mice
T Iglesias, JM Irache, M Butinar, B Turk, AL de Cerain… - International Journal of Pharmaceutics 2017
... Slides were then examined by a fluorescent microscope (Nikon Eclipse 50 i, Japan) using the image analysis system Comet assay IV (Perceptive instruments), scoring 75 comets per gel (2 gels/slide, 150 comets/sample). Percentage ...

Sodium fluoride adversely affects ovarian development and reproduction in Drosophila melanogaster
BS Khatun, P Rajak, M Dutta, S Roy - Chemosphere, 2017
... microscope at 400X magnification. Images were scored randomly using 'Comet Assay IV' software of Perceptive Instruments. Duplicate sets of slides were prepared and examined for each treatment category. 2.12. Statistical analysis. ...

In situ biomonitoring of air quality in rural and urban environments of Mexico Valley through genotoxicity evaluated in wild plants
S Gómez-Arroyo, J Cortés-Eslava, P Loza-Gómez… - Atmospheric Pollution …, 2017
... Slides were then examined by a fluorescent microscope (Nikon Eclipse 50 i, Japan) using the image analysis system Comet assay IV (Perceptive instruments), scoring 75 comets per gel (2 gels/slide, 150 comets/sample). Percentage ...

Cytotoxicity and genotoxicity of fenoxaprop-p-ethyl and fluzifob-p-butyl herbicides
B Ulus, M Çeliksöz, G Özhan - 2017
... DNA was stained with ethidium bromide (20 mg/mL) just before slide examination under a fluorescent microscope (Olympus, Japan) at 400 magnifi- cation by using an automated image analysis system (Comet Assay IV, Perceptive Instruments, UK). ...

A type I combi-targeting approach for the design of molecules with enhanced potency against BRCA1/2 mutant- and O6-methylguanine-DNA methyltransferase (mgmt)- expressing tumour cells
ZS Mouhri, E Goodfellow, B Jean-Claude - BMC Cancer, 2017
Comets were visualized at 400X magnification and DNA damage was quantified using Comet Assay IV software to calculate tail moments...

In Vitro Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell
ZS Mouhri, E Goodfellow, B Jean-Claude - BMC Cancer, 2017
... The number of DNA breaks were scored under a fluorescent microscope (Olympus BX53, Olympus, Tokyo, Japan) at 400 magnification using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...

Integrated assessment of toxic effects of maghemite (?-Fe2O3) nanoparticles in zebrafishs
RAR Villacis, S José Filho, B Piña, RB Azevedo… - Aquatic Toxicology, 2017
... bromide (20 µg/mL) and analyzed using a fluorescence microscope (ZEISS Axioskop 2-HAL100, at 400× magnification), fitted with a 510–560 nm filter and a 590 nm blocking filter; and the Comet IV Lite v4.3 system image analysis software (Perceptive Instruments, Suffolk, UK). ...

Dexrazoxane averts idarubicin-evoked genomic damage by regulating gene expression profiling associated with the DNA damage-signaling pathway in BALB/c mice
SM Attia, AY Alshahrani, MA Al-Hamamah, MM Attia… - Toxicological Sciences, 2017
... a Nikon fluorescence microscope. Two slides for each mouse were prepared, and 100 cells were randomly selected from each slide and subjected to image analysis by Comet Assay IV software (Perceptive Instruments, Suffolk, England). The level of DNA damage was ...

Vectorization by nanoparticles decreases the overall toxicity of airborne pollutants
R Carpentier, A Platel, H Maiz-Gregores, F Nesslany… - PLOS ONE, 2017
The comet assay was performed under alkaline conditions (pH>13) as described by ... camera to Comet Image Analysis System software (version 4, Perceptive Instruments Ltd., Haverhill

Kinetics of poly (ADP-ribosyl) ation, but not PARP1 itself, determines the cell fate in response to DNA damage in vitro and in vivo
H Schuhwerk, C Bruhn, K Siniuk, W Min, S Erener… - Nucleic Acids Research, 2017
the samples were analysed using an Axioplan 2 microscope (Zeiss) equipped with a stingray camera (Allied Vision Technologies, Stadtroda, Germany) operated by Comet Assay IV software (Perceptive instruments, Edmonds, UK).

The RIR motif in the scaffold protein XRCC1 mediates a low-affinity interaction with polynucleotide kinase/phosphatase (PNKP) during DNA single-strand break repair
C Breslin, RS Mani, M Fanta, N Hoch, M Weinfeld… - Journal of Biological …, 2017
... media for the desired repair period at 37?C. Cells (100 per data point) were then analysed by alkaline comet assay as previously described (44) using Comet Assay IV software (Perceptive Instruments). Steady-state Fluorescence ...

Baseline and oxidative DNA damage in marine invertebrates
A Sahlmann, R Wolf, TF Holth, J Titelman, K Hylland - Journal of Toxicology and …, 2017
... Fluorescence was read at 520/610 nm excita- tion/emission. Fifty cells/gel were scored and DNA damage, in terms of DNA strand breaks, was quan- tified using Comet Assay IV software (version 4.3; Perceptive Instruments; Bury St Edmunds, UK). Statistics ...

Environmentally realistic exposure to weathered North Sea oil: Sublethal effects in Atlantic cod (Gadus morhua) and turbot (Scophthalmus maximus)
TF Holth, A Storset, AL Ribeiro, Á Ólafsdóttir… - Journal of Toxicology and …, 2017
... A minimum of 30 cells and up to 50 cells was scored per sample, and sample value set to the median tail inten- sity. Comet Assay IV (Perceptive Instruments, UK) was used for scoring. Cells in gel edges were not included in the analysis, as described by Tice et al. (2000). ...

Genotoxic effects of high dose rate X-ray and low dose rate gamma radiation in ApcMin/+ mice
A Graupner, DM Eide, DA Brede, M Ellender… - Environmental and Molecular Mutagenesis, 2017
“Fifty randomly chosen comets per replicate (three technical replicates per mouse) were scored using 20× magnification with an Olympus BX51 microscope (light source: X-Cite® Series 120Q, Lumen Dynamics, Mississauga, ON, Canada; camera: BASLER, A312f-VIS, Vision Technologies, Germany) and the Comet IV analysis software (Perceptive Instruments, Bury St. Edmunds, UK) was used to quantify the relative amount of fluorescing DNA in the comet tail vs. that of the whole comet (% tail DNA) as a measure of the level of DNA lesions in the individual cell.”
“The raw data of the SCGE was processed using the Comet Assay Spreadsheet Generator Version 1.3.1 (Perceptive Instruments)."

Vitamin C partially prevents reproductive damage in adult male rats exposed to rosuvastatin during prepuberty
GAA Leite, TM Figueiredo, TL Pacheco, M Sanabria… - Food and Chemical …, 2017
... ... A total of 100 randomly selected nucleoids per animal was analyzed under 400 × magnification with a fluorescence microscope connected to an image analysis system (Comet Assay IV, Perceptive Instruments; Suffolk, Haverhill, UK). ...

Evaluating the potential genotoxicity of phthalates esters (PAEs) in perfumes using in vitro assays
I Al-Saleh, T Al-Rajudi, G Al-Qudaihi, P Manogaran - Environmental Science and Pollution Research, 2017
... At least 25 randomly selected cells were scored on each slide using the Comet Assay IV for Windows imaging system with a monochrome CCD IEEE1394 FireWire video camera (Perceptive Instruments, Halstead, UK)...

Parental gamma irradiation induces reprotoxic effects accompanied by genomic instability in zebrafish (Danio rerio) embryos
S Hurem, T Gomes, DA Brede, EL Hansen, S Mutoloki… - Environmental Research, 2017
... Fifty randomly chosen cells per replicate (150 cells per biological replicate, total 450 cells per dose rate) were scored using the Comet IV analysis software (Perceptive Instruments Ltd., Bury St. Edmunds, UK). Tail ...

Genotoxic effects in transformed and non-transformed human breast cell lines after exposure to silver nanoparticles in combination with aluminium chloride, butylparaben or di-n-butylphthalate
J Roszak, K Domeradzka-Gajda, A Smok-Pieniazek… - Toxicology in Vitro, 2017
... were stained with DAPI (5 µg/mL Sigma-Aldrich, #D-9542) and 50 cells from each slide were analyzed using an Olympus fluorescence microscope (a BX40 instrument; Olympus, Tokyo, Japan) equipped with an image analysis system (Comet IV, Perceptive Instruments, UK). ...

The herbicides trifluralin and tebuthiuron have no genotoxic or mutagenic potential as evidenced by genetic tests
MF Franco-Bernardes, OP Rocha, LC Pereira… - Environmental Science and …, 2017
... Analysis was conducted under an Olympus Bx50 fluorescence microscope (Olympus America INC, Melville, NY), at 40× magnification. The Comet IV (Perceptive Instruments Ltd., United Kingdom) software was employed. For ...

Investigation of full-scale ozonation at a municipal wastewater treatment plant using a toxicity-based evaluation concept
F Itzel, L Gehrmann, H Bielak, P Ebersbach… - Journal of Toxicology and …, 2017
... For each sample, 50 data points were measured per sample in one replicate. Olive Tail Moments (OTMs) with standard error of mean (SEM) were determined using the Comet Assay 4 software (Perceptive Instruments Ltd., Bury St Edmunds, UK). ...

Toxicological assessment of two silane-modified clay minerals with potential use as food contact materials in human hepatoma cells and Salmonella typhimurium strains
S Maisanaba, M Llana-Ruíz-Cabello, S Pichardo… - Applied Clay Science, 2017
.... out. Finally, DNA was stained with SYBR Gold nucleic acid gel stain. A fluorescence microscope (Olympus BX61) with comet assay IV software (Perceptive Instruments, UK) was used to score at least 100 cells per sample. The ...

Genotoxic potential of diesel exhaust particles from the combustion of first-and second-generation biodiesel fuels—the FuelHealth project
M Kowalska, A Wegierek-Ciuk, K Brzoska… - Environmental Science and …
... Image analysis of data was performed by the Comet Assay IV image analysis system (Perceptive Instruments, UK). Data for 75 randomly selected comets per point were analyzed. Percent of DNA in comet's tail was chosen as a measure of DNA damage. ...

AN-7, a butyric acid prodrug, sensitizes cutaneous T-cell lymphoma cell lines to doxorubicin via inhibition of DNA double strand breaks repair
L Moyal, N Goldfeiz, B Gorovitz, A Rephaeli, E Tal… - Investigational New Drugs, 2017
... The cells were scored under a fluorescence mi- croscope (Nikon ECLIPSE 55i, Tokyo, Japan) at ×10 magni- fication, and the scores were analyzed using Comet IV soft- ware (Perceptive Instruments, Bury St Edmonds, UK). ...

Evaluation of distribution, redox parameters, and genotoxicity in Wistar rats co-exposed to silver and titanium dioxide nanoparticless
AC Martins Jr, LF Azevedo, CC de Souza Rocha… - Journal of Toxicology and …, 2017
...Tail intensity (% DNA in tail) was analyzed in 150 nucleoids per animal using Comet Assay IV soft- ware (Perceptive Instruments, Suffolk, UK) and a fluorescence microscope (Axiostarplus, Zeiss) equipped with 515–560 nm excitation and 590 nm barrier filters. ...

TLR9 stimulation of B-cells induces transcription of p53 and prevents spontaneous and irradiation-induced cell death independent of DNA damage responses. Implications for Common variable immunodeficiency.
KL Holm, RG Syljuåsen, G Hasvold, L Alsøe, H Nilsen… - PloS one, 2017
... After neutralization with PBS and staining with SYBRGold (Invitrogen, Carlsbad, CA, USA), slides were scored (50 nucleoids per gel) using the Comet Assay IV image analysis program (Perceptive Instruments, Bury St Edmunds, UK). Quantitative mRNA analysis. ...

t-BuOOH induces ferroptosis in human and murine cell lines
C Wenz, D Faust, B Linz, C Turmann, T Nikolova… - Archives of Toxicology, 2017
... Neutral comet assay The cells were harvested and subjected to a neutral Comet assay. ... At least 50 cells/slide were scored using the Comet IV software (Perceptive Instruments Ltd., Bury St Edmunds, UK). Statistical analyses ...

The Rev1 interacting region (RIR) motif in the scaffold protein XRCC1 mediates a low-affinity interaction with polynucleotide kinase/phosphatase (PNKP) during DNA single-strand break repair
C Breslin, RS Mani, M Fanta, N Hoch, M Weinfeld… - Journal of Biological …, 2017
... rinsed in ice-cold PBS and incubated, where appropriate, in fresh drug-free media for the desired repair period at 37 °C. Cells (100 per data point) were then analyzed by alkaline comet assay as previously described (44) using Comet Assay IV software (Perceptive Instruments). ...

Genome size and sensitivity to DNA damage by X-rays—plant comets tell the story
J Einset, AR Collins - Mutagenesis, 2017
... genome sizes. Analysis of comets was performed as described with 50 comets scored per sample. DNA damage was measured as % tail DNA with the Comet Assay IV image analysis program (Perceptive Instruments). % Tail DNA ...

Next-Generation Magnetic Nanocomposites: Cytotoxic and Genotoxic Effects of Coated and Uncoated Ferric Cobalt Boron (FeCoB) Nanoparticles In Vitro
K Netzer, G Jordakieva, AM Girard, AC Budinsky… - Basic & Clinical Pharmacology & …
... Image analysis was performed using 'Comet Assay IV' software (Perspective Instruments, UK) and Axiophot fluorescence microscope (NIKON Japan 520490) attached to a Stingray camera (Allied Vision Technologies, Newburyport, MA, USA). ...

ROS generation and JNK activation contribute to 4-methoxy-TEMPO-induced cytotoxicity, autophagy, and DNA damage in HepG2 cells
Z Zhang, Z Ren, S Chen, X Guo, F Liu, L Guo, N Mei - Archives of Toxicology, 2017
... three independent exposures were analyzed. The percent- age of DNA in Comet tails was calculated using the Comet Assay IV digital analysis system and software (Perceptive Instruments; Edmunds, UK). Western blot analysis ...

Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
J Jovanovic, S Kolarevic, A Miloškovic, N Radojkovic… - Science of The Total …, 2017
... 590 nm). For scoring nucleoids, Comet IV Computer Software (Perceptive Instruments, UK) was used. The tail intensity (TI% - a percentage of DNA in the tail of the comet) was chosen as a parameter of DNA damage. For each ...

Genotoxicity of airborne PM2. 5 assessed by salmonella and comet assays in five cities of the Emilia-Romagna (Italy) mutagenicity monitoring network
KC Bocchi, C Bazzini, F Fontana, G Pinto, F Cassoni - Environmental and Molecular
... Louis, MO), duplicate slides were prepared for each dose and, in each slide, 100 randomly selected nuclei (200 cells per dose at 250× magnification) were acquired and analyzed with specific image analyser software (Comet Assay IV; Perspective Instruments, Suffolk, UK). ...

Comparative In Vitro Toxicity Evaluation of Heavy Metals (Lead, Cadmium, Arsenic, and Methylmercury) on HT-22 Hippocampal Cell Line
V Karri, V Kumar, D Ramos, E Oliveira… - Biological Trace Element …, 2017
... The slides were stained with DAPI (4', 6-diamidino-2- phenylindole), inspected visually, and analyzed using the Nikon epifluorescence microscope, comet analysis scoring system (Comet assay - IV) software (Perceptive instrument, UK). ...

Evaluation of chlorpyrifos toxicity through a 28-day study: Cholinesterase activity, oxidative stress responses, parent compound/metabolite levels, and primary DNA damage in blood and brain tissue of adult male Wistar rats
N Kopjar, S Žunec, G Mendaš, V Micek, V Kašuba… - Chemico-Biological …, 2017
... 590 nm)... One well-trained scorer performed all of the comet measurements on coded/blinded slides. The level of DNA damage in individual cells was assessed with an image analysis system (Comet Assay IV, Perceptive Instruments Ltd., Suffolk, Halstead, UK) using an epifluorescence microscope (Olympus BX50, Japan) equipped with appropriate filters, under 200× magnification. A total of 300 randomly selected comets per each rat (i.e. 1500 comets per each tested dose) were measured on replicate slides in two independent evaluations. Tail intensity (i. e. DNA % in tail) and tail length were chosen as indicators of DNA damage...

Effects of various wavelengths of light on physiological stress and non-specific immune responses in black rockfish Sebastes schlegelii subjected to water temperature change
CY Choi, TH Kim, YJ Choi, JY Choi, SY Oh, BS Kim - Fisheries Science, 2017
... 105 cells/mL) were examined using a Comet Assay Reagent Kit for single-cell gel elec- trophoresis (Trevigen, Gaithersburg, MD ... migration from the head) and % DNA in the tail (tail intensity/total intensity × 100) using the Comet Assay IV image analysis software (version 4.3.2 ...

Diclofop-methyl: A phenoxy propionate herbicide with multiple toxic effects in mouse embyro fibroblast (NIH/3T3) cell line.
M ÇELIKSÖZ, B ULUS, E ÖZTAS, G ÖZHAN - Marmara Pharmaceutical Journal, 2017
... bromide. The number of DNA breaks was scored under a fluorescent microscope (Olympus BX53, Tokyo, Japan) at 400X using an automated image analysis system (Comet Assay IV, Perceptive Instruments, Suffolk, UK). DNA ...

In Vitro Cytotoxicity and Genotoxicity of Furia ®180 SC (Zeta-cypermethrin) and Bulldock 125®SC (ß-Cyfluthrin) pyrethroid insecticides in human peripheral blood lymphocytes
ME Calderón-Segura, S Gómez-Arroyo… - Toxicology Mechanisms and …, 2017
... 590 nm)… To visualize the DNA damage, the slides were observed at 40X magnification using Comet IV Software (Perceptive Instruments). Three parameters were used to determine genotoxicity: a) tail length (the distance between the first and last DNA fragments), b) …

Cellular responses of human astrocytoma cells to dust from the Acheson process: An in vitro study
YJ Arnoldussen, TK Ervik, B Berlinger, I Kero… - NeuroToxicology, 2017/em>
... Slides were then examined under a Nicon fluorescent microscope using Comet assay IV software (Perceptive Instruments, UK), where mean% DNA in comet tails from 100 comets/gel was used as a measure of DNA strand breaks. ...

Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state
A El-Badawy, MA Ghoneim, MM Gabr, RA Salah… - Stem Cell Research & …, 2017
... Average tail moments from 50 cells per sample were measured using Comet Assay IV software (Perceptive Instruments, UK). Sphere formation assay. The sphere formation assay was performed as described previously with slight modifications [24]. ...

Identification of PMN-released Mutagenic Factors in a Co-culture Model for Colitis-associated Cancer
N Granofszky, M Lang, V Khare, G Schmid, T Scharl… - Carcinogenesis, 2017
... The DNA was stained with ethidium bromide (20µg/ml) and the percentage of DNA in tail was analyzed with a computer-aided system (Comet Assay IV, Perceptive Instruments, UK). Cell viability was determined with trypan blue (0.4%). ...

Capsaicin reduces genotoxicity, colonic cell proliferation and preneoplastic lesions induced by 1, 2-dimethylhydrazine in rats
BFR Caetano, MB Tablas, NEF Pereira, NA de Moura… - Toxicology and Applied …, 20177
… An epi-fluorescence microscope (Olympus BX-50, Japan) coupled to a CCD camera was used to score fifty random nucleoid/sample using the Comet Assay IV Image Analysis System (Perceptive Instruments, UK). All experiments were performed in duplicate …

Pendimethalin induces oxidative stress, DNA damage, and mitochondrial dysfunction to trigger apoptosis in human lymphocytes and rat bone-marrow cells
SM Ansari, Q Saquib, SM Attia, EM Abdel-Salam… - Histochemistry and Cell …, 2017
… Slides were then kept on ice packs for rapid solidification of gels and kept for overnight at 4 °C in lysing solution. Cells were then electrophoresed, neutralized and stained for DNA damage analysis on the Comet Assay IV software (Perceptive Instruments, Suffolk, UK) …

The toxin BjussuLAAO-II induces oxidative stress and DNA damage, upregulates the inflammatory cytokine genes TNF and IL6, and downregulates the apoptotic-related genes BAX, BCL2 and RELA in human Caco-2 cells
HART Machado, AF Aissa, DL Ribeiro, LC Hernandes… - International Journal of …, 2017
… One hundred nucleoids were analyzed per sample, with the aid of the Comet Assay IV™ software (Perceptive Instruments, Haverhil, UK). The tail intensity, ie, the percentage (%) of DNA in the tail, was used as parameter of DNA damage. 2.6 …

Gallic acid, a common dietary phenolic improves health associated biochemical parameters and prevents oxidative damage of DNA in type 2 diabetes patients: results of a placebo-controlled pilot study
F Ferk, M Kundi, H Brath, T Szekeres, H Al-Serori… - Molecular Nutrition & Food 2017
… (Nikon EFD-3, Tokyo, Japan) using a 25-fold magnification. DNA migration was determined with a computer aided comet assay image analysis system (Comet Assay IV, Perceptive Instruments, UK). The percentage of DNA in tail (% DNA) was monitored as an endpoint. In …

The secondary Fusarium metabolite aurofusarin induces oxidative stress, cytotoxicity and genotoxicity in human colon cells
K Jarolim, K Wolters, L Woelflingseder, G Pahlke… - Toxicology Letters, 2017
… Tail intensities of 50 cells per slide were counted with the Comet Assay IV software (Perceptive Instruments, England) on a fluorescence microscope (Axioskop A1, Zeiss, Germany). After analysis, the mean value of 50 counted cells was calculated. 2.10 …

Genotoxic and mutagenic properties of Ni and NiO nanoparticles investigated by comet assay, ?-H2AX staining, Hprt mutation assay and ToxTracker reporter cell lines
E Åkerlund, F Cappellini, S Di Bucchianico, S Islam… - Environmental and Molecular … 2017
The slides were stained in a 1:10,000 dilution of Sybr Green (Invitrogen) in TAE buffer for 15 min and scored with a fluorescence microscope (LeicaDMLB, Houston,TX) using the Comet assay IV software (Perceptive Instruments, Suffolk, UK). In each experiment 50 comets in duplicates on two different slides were evaluated for each sample.

Cocoplum (Chrysobalanus icaco L.) decreases doxorubicin-induced DNA damage and downregulates Gadd45a, Il-1ß, and Tnf-a in vivo
VP Venancio, MR Almeida, LMG Antunes - Food Research International, 2017
… The Tail Moment (product of the proportion of the tail's intensity and the displacement of the tail's center of mass relative to the center of the head) and Tail Intensity (% DNA in the tail) were evaluated using Comet Assay IV software (Perceptive Instruments, Suffolk, UK) at 200 …

Use of HuH6 and other human-derived hepatoma lines for the detection of genotoxins: a new hope for laboratory animals?
M Waldherr, M Mišík, F Ferk, J Tomc, B Žegura… - Archives of Toxicology, 2017
… As an endpoint, we determined the tail intensities (“% DNA in tail”) with a computer-aided system Comet Assay IV (Perceptive Instruments, Bury St Edmunds, UK), which is at present the most widely used parameter (Collins et al. 2008; Moller 2006) …