Papers from 2006

Below are a number of refereed papers from 2006 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include genotoxicity testing of materials used in dentistry; potentially harmful and beneficial dietary factors; biomonitoring; molecular epidemiology; photobiology; mechanistic studies and clinical.

2-Dodecylcyclobutanone, a radiolytic product of palmitic acid, is genotoxic in primary human colon cells and in cells from preneoplastic lesions
(2006) N. Knoll et al, Mut. Res. 594(1-2):10-19

A Protease Pathway for the Repair of Topoisomerase II-DNA Covalent Complexes
(2006) A. Zhang et al, Journal of Biological Chemistry 281(47):35997-36003

Alkaline comet assay study with breast cancer patients: evaluation of baseline and chemotherapy-induced DNA damage in non-target cells
(2006) N. Kopjar et al, Clin. Exp. Med. 6(4):177-190

Antimicrobial endodontic compounds do not modulate alkylation-induced genotoxicity and oxidative stress in vitro
(2006) D.A. Ribeiro et al, Oral Surg. 102(2):e32-e36

Antioxidant and antigenotoxic activities of Angelica keiskei, Oenanthe javanica and Brassica oleracea in the Salmonella mutagenicity assay and in HCT116 human colon cancer cells
(2006) D. Kwon et al, BioFactors 26(4):231-244

Biocompatibility of glass–ionomer cements using mouse lymphoma cells in vitro
(2006) D.A. Ribeiro et al, Oral Rehab. 33(12):912-917

Development and validation of a modified comet assay to phenotypically assess nucleotide excision repair
(2006) S. Langie et al, Mutagenesis 21(2):153-158

Dithiol Compounds at Low Concentrations Increase Arsenite Toxicity
(2006) K.Y. Jan et al, Toxicological Sciences 90(2):432-439

DNA damage and repair capacity by comet assay in lymphocytes of white-collar active smokers and passive smokers (non- and ex-smokers) at workplace
(2006) M. Fracasso et al, Toxicology Let. 167(2):131-141

DNA damage by ochratoxin A in rat kidney assessed by the alkaline comet assay
(2006) D. Želježic et al, Braz J Med Biol Res [online]

DNA repair contributes to the drug-resistant phenotype of primary acute myeloid leukaemia cells with FLT3 internal tandem duplications and is reversed by the FLT3 inhibitor PKC412
(2006) C. Seedhouse et al, Leukemia 20:2130–2136

Effects of co-exposure to extremely low frequency (50 Hz) magnetic fields and xenobiotics determined in vitro by the alkaline comet assay
(2006) M. Villarini et al, Sci. Total Env. 361(1-3):208-219

Evaluation of DNA damage in white blood cells of healthy human volunteers using the alkaline comet assay and the chromosome aberration test
(2006) N. Kopjar, D. Zeljezic and V. Garaj-Vrhovac, Acta biochimica Polonica 53(2):321-336

Evaluation of genetic damage in human peripheral lymphocytes exposed to mineral trioxide aggregate and Portland cements
(2006) M.G. Braz et al, Journal of Oral Rehabilitation 33(3):234-239

Ex vivo biocompatibility tests of regular and white forms of mineral trioxide aggregate
(2006) D.A. Ribeiro et al, International Endodontic Journal 39(1):26-30

Genotoxic effects of myosmine in a human esophageal adenocarcinoma cell line
(2006) S. Vogt, K. Fuchs and E. Richter, Toxicology 222(1):71-79

Genotoxicity and cytotoxicity of glass ionomer cements on Chinese hamster ovary (CHO) cells
(2006) D.A. Ribeiro et al, Journal of Materials Science: Materials in Medicine 17(6):495-500

Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro
(2006) D. Ribeiro et al, Oral Surg. 101(2):258-261

Genotoxicity in primary human peripheral lymphocytes after exposure to regular and white mineral trioxide aggregate
(2006) G. da Silva et al, Oral Surg. 102(5):e50-e54

Glutathione S-transferase polymorphisms and ochratoxin A toxicity in primary human urothelial cells
(2006) S. Lebrun et al, Toxicology 224(1-2):81-90

Hemoglobin and hemin induce DNA damage in human colon tumor cells HT29 clone 19A and in primary human colonocytes
(2006) M. Glei et al, Toxicological Sciences 90(2):432-439

Hg(II) Exposure Exacerbates UV-Induced DNA Damage in MRC5 Fibroblasts: A Comet Assay Study
(2006) W. Bradfield et al, Journal of Environmental Science and Health Part A 41(2):143-148

In vitro cytotoxic and non-genotoxic effects of gutta-percha solvents on mouse lymphoma cells by single cell gel (comet) assay
(2006) D.A. Ribiero et al, Braz. Dent. J. 17(3)

Influence of biotransformation on trophic transfer of the PAH, fluoranthene
(2006) A.Palmqvist et al, Aqua. Tox. 80(3):309-319

Influence of the SCGE protocol on the amount of basal DNA damage detected in the Mediterranean mussel, Mytilus galloprovincialis
(2006) N. Machella et al, Env. Mol. Mutagen 47(8):579-586

Investigation of cytotoxic, genotoxic, mutagenic, and estrogenic effects of the flame retardants tris-(2-chloroethyl)-phosphate (TCEP) and tris-(2-chloropropyl)-phosphate (TCPP) in vitro
(2006) W. Föllmann and J. Wober, Toxicology Let.161(2):124-134

Lack of DNA damage induced by fluoride on mouse lymphoma and human fibroblast cells by single cell gel (comet) assay
(2006) D.A. Ribeiro et al, Brazilian Dental Journal 17(2):91-94

Modulation of oxidative cell damage by reconstituted mixtures of phenolic apple juice extracts in human colon cell lines
(2006) S. Schaefer, M. Baum, G. Eisenbrand and C. Janzowski, Molecular Nutrition and Food Research 50(4-5):413-417

Multifunctional polyvinylpyrrolidinone-polyacrylic acid copolymer hydrogels for biomedical applications
(2006) D. M. Devine et al, Int. J Pharma. 326(1-2):50-59

Mutagenicity of cytochrome P450 2E1 substrates in the Ames test with the metabolic competent S. typhimurium strain YG7108pin3ERb5
(2006) B.Emmert et al, Toxicology 228(1):66-76

Oxidative stress as DNA damage in different transgenic tobacco plants
(2006) A. Mancini et al, Plant Sci. 170(7):845-852

Photo-chemically induced DNA effects in the comet assay with epidermal cells of SKH-1 mice after a single oral administration of different fluoroquinolones and 8-methoxypsoralen in combination with exposure to UVA
(2006) U.Wirnitzer et al, Mut. Res. 609(1):1-10

Standard and Fpg-modified comet assay in kidney cells of ochratoxin A- and fumonisin B1-treated rats
(2006) A.Domijan et al, Toxicology 222(1-2):53-59

Study of DNA damage induced by dental bleaching agents in vitro
(2006) D.A. Ribeiro et al, Brazilian Oral Research 20(1):47-51

The hollow fibre model - facilitating anti-cancer pre-clinical pharmacodynamics and improving animal welfare
(2006) M. Suggitt et al, Int. J Oncol. 29(6):1493-1499

The neutral diffusion assay as an estimate of cytotoxicity in the comet assay
(2006) C.Rider et al, Toxicology 226(1):58-59

The Parp-1 Inhibitor INO-1001 Facilitates Hemodynamic Stabilization Without Affecting DNA Repair in Porcine Thoracic Aortic Cross-Clamping-Induced Ischemia/Reperfusion
(2006) B. Hauser et al, Shock 25(6):633-640

The protective effect of L-carnitine in peripheral blood human lymphocytes exposed to oxidative agents
(2006) C.L. Garcia et al, Mutagenesis 21(1):21-27

Quotes

Everything has gone very well with the system and we are very pleased with it.

Scientist, University, UK

We have had no problems so far, and we are very pleased with the system.

Senior Researcher, Public Health Agency, Finland

Just to let you know we have started the validation study for Comet Assay IV and it has been going very well! We ran a comet assay last week and scored the slides with no problems at all. I am very pleased with the system and it will certainly make a difference when we run some comet studies this year!

Study Director, Food & Household Products Company, UK

I love the software. Keep up the good work over there.

Study Director, Contract Research Organisation, USA

It's an amazing program! Really useful!

Research Scientist, University, Brazil

We are using the Comet Assay IV a lot (and our use is increasing). It's working very well.

Professor, Children's Research Hospital, USA

Perfect and very clear installation and training.

Study Director, Contract Research Organisation, Europe

We have found the Comet Assay IV software very useful and convenient.

Research Scientist, Food Safety Agency, France

It is actually working great.

Professor, University, USA

We are very satisfied with your software.

Scientist, Cancer Research Institute, Slovakia

I'm really a fan of your system :)

Genetic Toxicologist, Global Pharmaceutical Company

Thanks for your attention and great service. I am so glad that we have Comet IV.

Study Director, Contract Research Organisation, USA

The system is working great.

Senior Research Scientist, Cancer Research Institute, UK

We have installed the software and are using it without any problem. It's really simple! My PhD students are very happy with it.

Professor, University, Spain

We are impressed indeed! It has saved us quite a lot of time and pains.

Director, Toxicology Research Centre, Spain

Excellent, the training was very helpful and specific to in-house technicalities.

Lecturer, University, UK

We are very happy with everything, it is easy to work with your software.

Senior Scientist, National Health Institute, Portugal

Yes, it is very useful and we are in the process of writing a paper also from the data derived from Comet Assay IV.

Assistant Professor, University, USA

Everything has gone very well with the system and we are very pleased with it.

Professor, University, UK

The installation was quick and all the details were described and easily understandable for the technicians.

Head of Department, Global Chemical Company, Germany

Comet Assay IV software is in use. Currently our comet analysis is based on tail moment measurement and I must say that everything works properly.

Researcher, Cancer Research Institute, Slovakia

...have been using it now for a couple of weeks and it seems to be working great. Very user friendly - I love it as does my intern!

Staff Chemist, Marine Laboratory, USA

It is, indeed, much quicker than the old image analysis system that we had. We are very pleased with it.

Senior Researcher, Public Health Agency, Finland

We started using Comet Assay IV and it works well...

Researcher, Nuclear Agency, France

I have been using the Comet Assay IV Software. It is great! During my software validation, I realized how much easier and precise it is compared to our old software. It makes analyzing the comet slides more enjoyable

Scientist, Medical Research Centre, USA

Perfect and very clear installation and training!

Researcher, Contract Testing Laboratory, Europe

Excellent, perfectly straightforward.

Scientist, University, UK

We have just recently utilized the system and are extremely pleased.

Assistant Professor, University, USA

So it's working out fine and we're very happy!

Toxicologist, Health Agency, Canada

The Comet Assay IV system we already installed and is working perfectly.

Scientist, Environmental Institute, Norway

I don't have any questions about the software as it has worked quite well for us...so far we are very happy with the software.

Research Scientist, University, Australia

We're now using the software and it works great. It has cut our time for comets by at least half.

Post Doctoral Fellow, National Institute of Health, USA

The system is up and running. Everybody is impressed with the speed of scoring slides.

Researcher, University, South Africa

We are really very happy with the comet analysis system.

Research Scientist, University, Austria

...we are "happy users" of the software

Senior Researcher, Risk Assessment Institute, Germany

...Everything is fine with the Comet Assay IV system...and I'm pretty happy about this new software with just clicking on the cells instead of drawing a frame around them

Researcher, Cancer Research Organisation, UK

Total change in our lab since you came (with Comet Assay IV of course...)! thanks... :-)

Postgraduate Researcher, University, France

The system was set up last month, and so far it works very well. Both my boss and I are very satisfied with it and like it very much.

Researcher, University Hospital, Germany

All is going well and the more I use the software the more I love the way it's so simple and intuitive...the comet software is running well and in increasing use.

Scientist, University, UK

The current Comet Assay IV system works without any trouble…we are pleased with the system.

Scientist, Chemical Company, Japan

So far, I'm very happy with the system.

Research Lecturer, University, UK