Papers from 2009

Below are a number of refereed papers from 2009 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include research and testing in nutrition, medicine, biomonitoring and genetic toxicology:

Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells
(2009) K. Gerloff et al, Nanotoxicology 3(4): 355-364

Delphinidin Modulates the DNA-Damaging Properties of Topoisomerase II Poisons
(2009) M. Esselen et al, Chem. Res. Toxicol. 22(3):554-564

Genotoxicity evaluation of locally produced dental porcelain – An in vitro study using the Ames and Comet assays
(2009) M. Noushad et al., Toxicol In Vitro. 23(6):1145-50

Nanoparticles can cause DNA damage across a cellular barrier
(2009) G. Bhabra et al., Nature Nanotech. (e-pub)

SiC nanoparticles cyto-and genotoxicity to Hep-G2 cells
(2009) S.Barillet et al J. Phys.: Conf Ser. 170 012016

β-Carotene metabolites enhance inflammation-induced oxidative DNA damage in lung epithelial cells
(2009) YG van Helden et al, Free Radic Biol Med.46(2):299-304

A class of DNA-binding peptides from wheat bud causes growth inhibition, G2 cell cycle arrest and apoptosis induction in HeLa cells
(2009) L. Mancinelli et al., Mol Cancer. 8(1):55

A Novel Liquid Multi-Phytonutrient Supplement Demonstrates DNA-Protective Effects
(2009) B. Baechler et al., Plant Foods Hum Nutr. 64(2):81-5

A unique program for cell death in xylem fibers of Populus stem
(2009) C.L. Courtois-Moreau et al, The Plant Journal 58(2):260-274

Alternariol acts as a topoisomerase poison, preferentially affecting the IIalpha isoform.
(2009) M. Fehr et al, Mol Nutr Food Res. 53(4):441-51

Antioxidant activities of major thyme ingredients and lack of (oxidative) DNA damage in V79 Chinese hamster lung fibroblast cells at low levels of carvacrol and thymol
(2009) U. Undeger et al, F. Chem. Toxicol. 47(8):2037-2043

Application of the comet assay and detection of DNA damage in haemocytes of medicinal leech affected by aluminium pollution: A case study
(2009) Z Mihaljević et al. Environmental Pollution 157(5):1565-1672

Assessment of DNA sensitivity in peripheral blood leukocytes after occupational exposure to microwave radiation: the alkaline comet assay and chromatid breakage assay
(2009) V.Garaj-Vrhovac & V.Oreščanin Cell Biol Toxicol 25(1):33–43

Assessment of genotoxic risks in Croatian health care workers occupationally exposed to cytotoxic drugs: A multi-biomarker approach
(2009) N. Kopjar et al, Int J Hyg Environ Health. 212(4):414-31

C-1027, A Radiomimetic Enediyne Anticancer Drug, Preferentially Targets Hypoxic Cells
(2009) T.A. Beerman et al, Cancer Research 69(2):593-598

Cellular uptake of platinum nanoparticles in human colon carcinoma cells and their impact on cellular redox systems and DNA integrity.
(2009) J. Pelka et al., Chem Res Toxicol. 22(4):649-59

Chronic exposure to MDMA (ecstasy) increases DNA damage in sperm and alters testes histopathology in male rats
(2009) M. Barenys et al., Toxicol Lett. 191(1):40-6

Chronic renal failure induces genetic instability in multiple organs of Wistar rats
(2009) D.A. Ribeiro et al, Eur J Clin Invest. 39(4):289-95

Citotoxicity status of electroplating wastewater prior/after neutralization/purification with alkaline solid residue of electric arc furnace dust
(2009) V. Orescanin et al, J Environ Sci Health A Tox Hazard Subst Environ Eng. 44(3):273-8

Combination of in vitro bioassays for the determination of cytotoxic and genotoxic potential of wastewater, surface water and drinking water samples
(2009) B. Zegura et al, Chemosphere 75(11):1453-1460

Comet Assay in Evaluating DNA Damage Associated With Ischaemia-Reperfusion Injury in Patients Undergoing Coronary Surgery
(2009) B. Karahalil et al., Arh Hig Rada Toksikol 60(3):307-315

Dietary zinc restriction and repletion affects DNA integrity in healthy men
(2009) Y. Song et al, Am J Clin Nutr. 90(2):321-8

Distinct effects of acute and chronic sleep loss on DNA damage in rats
(2009) M.L. Andersen et al, Prog Neuropsychopharmacol Biol Psychiatry. 33(3):562-7

DNA damage detected by the alkaline comet assay in the liver of mice after oral administration of tetrachloroethylene
(2009) H. Cederberg et al, Mutagenesis (e-pub)

DNA damage in horticultural farmers: a pilot study showing an association with organophosphate pesticide exposure
(2009) K.M. Atherton et al., Biomarkers. (e-pub)

DNA single- and double-strand breaks by alkaline- and immuno-comet assay in lymphocytes of workers exposed to styrene
(2009) M.E. Fracasso et al, Toxicology Letters 185(1):9-25

Drinking water quality: an in vitro approach for the assessment of cytotoxic and genotoxic load in water sampled along distribution system.
(2009) F. Maffei et al., Environ Int. 35(7):1053-61

Elevated metals compromise repair of oxidative DNA damage via the base excision repair pathway: implications of pathologic iron-overload in the brain on integrity of neuronal DNA
(2009) H. Li et al., J Neurochem. 110(6):1774-83

Enhancement of chemically induced reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells by 872 MHz radiofrequency radiation
(2009) J. Luukkonen et al, Mutat. Res. 662(1-2):54-58

Epigenetic Reprogramming of IGF1 and Leptin Genes by Serum Deprivation in Multipotential Mesenchymal Stromal Cells
(2009) C. Sanchez et al, Stem Cells 27(2):375-382

Evaluation of DNA damage and lipoperoxidation of propofol in patients undergoing elective surgery
(2009) M.G. Braz et al., Eur J Anaesthesiol. 26(8):654-60.

Evaluation of genome damage and its relation to oxidative stress induced by glyphosate in human lymphocytes in vitro.
(2009) M. Mladinic et al, Environ Mol Mutagen. 50(9):800-7

Evaluation of radioprotective effects of propolis and its flavonoid constituents: in vitro study on human white blood cells
(2009) V Benkovic et al, Phyto. Res. 23(8):1159-1168

Evaluation of the cytogenetic damage induced by the organophosphorous insecticide acephate
(2009) D. Ozkan et al, Cytotechnology 59(2):73-80

Evaluation of the Cytogenetic Status of Human Lymphocytes After Exposure to a High Concentration of Bee Venom In Vitro
(2009) V. Garaj-Vrhova, G. Gajski, Arh Hig Rada Toksikol 60(1):27-34

Expression of stress-response ATF3 is mediated by Nrf2 in astrocytes
(2009) K.H. Kim et al., Nucl. Acids Res. (e-pub)

Fifty-gigahertz microwave exposure effect of radiations on rat brain.
(2009) K.K. Kesari & J. Behari, Appl Biochem Biotechnol. 158(1):126-39

Ganglioside GT1b protects human spermatozoa from hydrogen peroxide-induced DNA and membrane damage
(2009) M. Gavella et al, Int J Androl. (e-pub)

Genetic biomonitoring of an urban population exposed to mutagenic airborne pollutants
(2009) M.V. Coronas et al, Env. Int. 35(7):1023-1029

Genotoxic effects of anaesthetics in operating theatre personnel evaluated by the comet assay and micronucleus test
(2009) R.Rozgaj et al, Int. J. Hyg. Environ. Health 212(1):11-17

Genotoxic effects of neutrophils and hypochlorous acid
(2009) N. Gungor et al., Mutagenesis (e-pub)

Genotoxicity and cytotoxicity in multiple organs induced by titanium miniplates in Wistar rats
(2009) R. Piozzi et al, J Biomed Res A 88(2):342-7

Genotoxicity of cigarette smoking in maternal and newborn lymphocytes
(2009) K. R. Carvalho de Assis et al, Mutat Res. 679(1-2):72-8

Geostatistical analysis of DNA damage in oysters, Crassostrea virginica, in Lavaca Bay, Texas
(2009) W. Bissett et al, Ecotox. 18(1):1573-3017

Health risk assessment by measuring plasma malondialdehyde (MDA), urinary 8-hydroxydeoxyguanosine (8-OH-dG) and DNA strand breakage following metal exposure in foundry workers
(2009) H.H. Liu et al, J. Haz. Mat. 170(2-3):699-704

Homologous recombination but not nucleotide excision repair plays a pivotal role in tolerance of DNA-protein cross-links in mammalian cells.
(2009) T. Nakano et al., J Biol Chem. 284(40):27065-76

In vitro assessment of genotoxic effects of electric arc furnace dust on human lymphocytes using the alkaline comet assay.
(2009) V.Garaj-Vrhovac et al, J Environ Sci Health A Tox Hazard Subst Environ Eng. 44(3):279-87

In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium
(2009) A. Gamal-Eldeen et al, Food Chem Toxicol. 47(6):1378-84

In vitro genotoxicity of root canal sealers
(2009) V. Brzovic et al, Int. Endod. J. 42(3):253-263

In vivo genotoxicity evaluation of a treated urban sewage sludge sample
(2009) M. de Lourdes Marzo Solano et al, Mutat. Res. 676(1-2):69-73

Induction of Macrophage Apoptosis by an Organochlorine Insecticide Acetofenate
(2009) M Zhao et al, Chem. Res. Toxicol. 22(3):504-510

Inhalation of formaldehyde does not induce systemic genotoxic effects in rats.
(2009) G. Speit et al., Mutat Res. 677(1-2):76-85

Interaction of ionizing radiation and ZRBA1, a mixed EGFR/DNA-targeting molecule.
(2009) M. Heravi et al., Anticancer Drugs. 20(8):659-67

Interactions of β-carotene and flavonoids on the secretion of pro-inflammatory mediators in an in vitro system
(2009) S.L. Yeh et al, Chem. Bio. Interactions 179(2-3):386-393

Low air levels of benzene: Correlation between biomarkers of exposure and genotoxic effects
(2009) M.E. Fracasso et al, Toxicology Letters (e-pub)

Measurement of oxidative DNA damage induced by mainstream cigarette smoke in cultured NCI-H292 human pulmonary carcinoma cells
(2009) F. Thorne et al, Mutat Res. 673(1):3-8

Molecular Signatures of N-nitroso Compounds in Caco-2 Cells: Implications for Colon Carcinogenesis
(2009) D.G.A.J. Hebels et al, Toxicol. Sci. 108(2):290-300

Neuroprotective effects of genistein and folic acid on apoptosis of rat cultured cortical neurons induced by β-amyloid 31-35
(2009) H.L. Yu et al, Br J Nutr. 102(5):655-62

Non-irradiated campherquinone induces DNA damage in human gingival fibroblasts
(2009) J. Volk et al., Dent Mater. 25(12):1556-63

Occupational exposure and DNA strand breakage of workers in bottom ash recovery and fly ash treatment plants
(2009) H.L. Chen et al., J Hazard Mater. 174(1-3):23-7

Oxidative stress and DNA damage in agricultural workers
(2009) G.E. Kisby et al, J Agromedicine 14(2):206-14

Partial hydrolysis enhances the inhibitory effects of konjac glucomannan from Amorphophallus konjac C. Koch on DNA damage induced by fecal water in Caco-2 cells
(2009) S.L. Yeh et al., Food Chemistry 119(2):614-618

Primary DNA Damage Assessed With the Comet Assay and Comparison to the Absorbed Dose of Diagnostic X-rays in Children
(2009) D. Milkovic et al, Int J Toxicol. 28(5):405-16.

Proliferative Activity In Vitro and DNA Repair Indicate that Adult Mouse and Human Sertoli Cells Are Not Terminally Differentiated, Quiescent Cells
(2009) E.A. Ahmed et al, Biol Reprod. 80(6):1084-91

Protective effect of linalool, myrcene and eucalyptol against t-butyl hydroperoxide induced genotoxicity in bacteria and cultured human cells
(2009) D. Mitic-Culafic et al, Food Chem. Toxicol. 47(1):260-266

Quartz-Containing Ceramic Dusts: In vitro screening of the cytotoxic, genotoxic and pro-inflammatory potential of 5 factory samples
(2009) C. Ziemann et al, J. Phys.: Conf. Ser 151 012022

RACK-1 overexpression protects against goniothalamin-induced cell death.
(2009) S.H. Inayat-Hussain et al., Toxicol Lett. 191(2-3):118-22

Radioprotective Effects of Honeybee Venom (Apis mellifera) Against 915-MHz Microwave Radiation–Induced DNA Damage in Wistar Rat Lymphocytes: In Vitro Study
(2009) G. Gajski, V.Garaj-Vrhovac Int. J. Toxicol. 28(2):88-98

Radioprotective Effects of Quercetin and Ethanolic Extract of Propolis in Gamma-Irradiated Mice
(2009) V. Benkovic et al., Arh Hig Rada Toksikol. 60(2):129-38

Repair of DNA damage induced by anthanthrene, a polycyclic aromatic hydrocarbon (PAH) without bay or fjord regions
(2009) C. Desler et al, Chem. Bio. Interactions 177(3):212-217

Responses of the European flounder Platichthys flesus to the chemical stress in estuaries: load of contaminants, gene expression, cellular impact and growth rate
(2009) E. Evrard et al., Biomarkers (e-pub)

Small-Molecule Drugs Mimicking DNA Damage: A New Strategy for Sensitizing Tumors to Radiotherapy
(2009) M. Quanz et al, Clin Cancer Res. 15(4):1308-16

Synergistic decrease of DNA single-strand break repair rates in mouse neural cells lacking both Tdp1 and aprataxin
(2009) S. El-Khamisy et al, DNA Repair 8(6):760-766

Synthesis, characterization and deepening in the comprehension of the biological action mechanisms of a new nickel complex with antiproliferative activity
(2009) A. Buschini et al, J. Inorg. Biochem. 103(5):666-677

The aldo-keto reductase AKR1C3 contributes to 7,12-dimethylbenz(a)anthracene-3,4-dihydrodiol mediated oxidative DNA damage in myeloid cells: Implications for leukemogenesis
(2009) J. Birtwistle et al, Mut. Res. 662(1-2):67-74

The emerging Fusarium toxin enniatin B: in-vitro studies on its genotoxic potential and cytotoxicity in V79 cells in relation to other mycotoxins
(2009) W. Follmann et al, Mycotox. Res. 25(1):11-19

The Fusarium toxin enniatin B exerts no genotoxic activity, but pronounced cytotoxicity in vitro
(2009) C. Behm et al, Mol Nutr Food Res. 53(4):423-30

Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in human lung cells.
(2009) K Bhattacharya et al, Particle and Fibre Toxicology. 6(1):17

Transcript profiling and DNA damage in the European eel (Anguilla anguilla L.) exposed to 7,12-dimethylbenz[a]anthracene.
(2009) P. Nogueira et al., Aquat Toxicol. 94(2):123-30

Usage of the standard and modified comet assay in assessment of DNA damage in human lymphocytes after exposure to ionizing radiation
(2009) M.Mikloš, G.Gajski, V.Garaj-Vrhovac, Radiol Oncol 43(2):97-107

Quotes

We are very satisfied with your software.

Scientist, Cancer Research Institute, Slovakia

We are really very happy with the comet analysis system.

Research Scientist, University, Austria

The current Comet Assay IV system works without any trouble…we are pleased with the system.

Scientist, Chemical Company, Japan

The system is working great.

Senior Research Scientist, Cancer Research Institute, UK

Everything has gone very well with the system and we are very pleased with it.

Scientist, University, UK

The Comet Assay IV system we already installed and is working perfectly.

Scientist, Environmental Institute, Norway

...Everything is fine with the Comet Assay IV system...and I'm pretty happy about this new software with just clicking on the cells instead of drawing a frame around them

Researcher, Cancer Research Organisation, UK

It's an amazing program! Really useful!

Research Scientist, University, Brazil

...we are "happy users" of the software

Senior Researcher, Risk Assessment Institute, Germany

The system is up and running. Everybody is impressed with the speed of scoring slides.

Researcher, University, South Africa

Excellent, the training was very helpful and specific to in-house technicalities.

Lecturer, University, UK

All is going well and the more I use the software the more I love the way it's so simple and intuitive...the comet software is running well and in increasing use.

Scientist, University, UK

Thanks for your attention and great service. I am so glad that we have Comet IV.

Study Director, Contract Research Organisation, USA

We have found the Comet Assay IV software very useful and convenient.

Research Scientist, Food Safety Agency, France

I have been using the Comet Assay IV Software. It is great! During my software validation, I realized how much easier and precise it is compared to our old software. It makes analyzing the comet slides more enjoyable

Scientist, Medical Research Centre, USA

I love the software. Keep up the good work over there.

Study Director, Contract Research Organisation, USA

Perfect and very clear installation and training.

Study Director, Contract Research Organisation, Europe

We are using the Comet Assay IV a lot (and our use is increasing). It's working very well.

Professor, Children's Research Hospital, USA

Perfect and very clear installation and training!

Researcher, Contract Testing Laboratory, Europe

We are very happy with everything, it is easy to work with your software.

Senior Scientist, National Health Institute, Portugal

...and the system is running great.

Medical Researcher, Cancer Research Institute, USA

The installation was quick and all the details were described and easily understandable for the technicians.

Head of Department, Global Chemical Company, Germany

Comet Assay IV software is in use. Currently our comet analysis is based on tail moment measurement and I must say that everything works properly.

Researcher, Cancer Research Institute, Slovakia

I'm really a fan of your system :)

Genetic Toxicologist, Global Pharmaceutical Company

The system was set up last month, and so far it works very well. Both my boss and I are very satisfied with it and like it very much.

Researcher, University Hospital, Germany

We have had no problems so far, and we are very pleased with the system.

Senior Researcher, Public Health Agency, Finland

Total change in our lab since you came (with Comet Assay IV of course...)! thanks... :-)

Postgraduate Researcher, University, France

Excellent, perfectly straightforward.

Scientist, University, UK

It is actually working great.

Professor, University, USA

I don't have any questions about the software as it has worked quite well for us...so far we are very happy with the software.

Research Scientist, University, Australia

It is, indeed, much quicker than the old image analysis system that we had. We are very pleased with it.

Senior Researcher, Public Health Agency, Finland

Yes, it is very useful and we are in the process of writing a paper also from the data derived from Comet Assay IV.

Assistant Professor, University, USA

So far, I'm very happy with the system.

Research Lecturer, University, UK

We're now using the software and it works great. It has cut our time for comets by at least half.

Post Doctoral Fellow, National Institute of Health, USA

So it's working out fine and we're very happy!

Toxicologist, Health Agency, Canada

We have installed the software and are using it without any problem. It's really simple! My PhD students are very happy with it.

Professor, University, Spain

We have just recently utilized the system and are extremely pleased.

Assistant Professor, University, USA

Just to let you know we have started the validation study for Comet Assay IV and it has been going very well! We ran a comet assay last week and scored the slides with no problems at all. I am very pleased with the system and it will certainly make a difference when we run some comet studies this year!

Study Director, Food & Household Products Company, UK

...have been using it now for a couple of weeks and it seems to be working great. Very user friendly - I love it as does my intern!

Staff Chemist, Marine Laboratory, USA

Everything has gone very well with the system and we are very pleased with it.

Professor, University, UK

We started using Comet Assay IV and it works well...

Researcher, Nuclear Agency, France