Papers from 2008

Below are a number of refereed papers from 2008 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include research and testing in nutrition, medicine, biomonitoring and genetic toxicology:

Alcohol induces DNA damage and the Fanconi anemia D2 protein implicating FANCD2 in the DNA damage response pathways in brain
(2008) S.L. Rulten et al, Alc. Clin. Exp. Res. 32(7):1186-1196

Analysis of DNA breaks, DNA damage response, and apoptosis produced by high NaCl
(2008) N.I. Dmitrieva et al, Am. J. Phys. Renal Phys. 295(1):1678-1688

Anthocyanin/Polyphenolic–Rich Fruit Juice Reduces Oxidative Cell Damage in an Intervention Study with Patients on Hemodialysis
(2008) T.M. Spormann et al, Canc. Epi. Biom. & Prev. 17(1):3372-3380

Application of cytogenetic endpoints and comet assay on human lymphocytes treated with atorvastatin in vitro
(2008) G. Gajski and V. Garaj-vrhovac J. Env. Sci & Health Pt.A 43(1):78-85

Assessment of genotoxic risks in Croatian health care workers occupationally exposed to cytotoxic drugs: A multi-biomarker approach
(2008) N. Kopjar et al, Int. J. Hyg. Env. Health [Epub]

Assessment of the genotoxicity of trichloroethylene and its metabolite, S-(1,2-dichlorovinyl)-L-cysteine (DCVC), in the comet assay in rat kidney
(2008) P. Clay Mutagenesis 23(1):27-33

Cellular protection from oxidative DNA damage by over-expression of the novel globin cytoglobin in vitro
(2008) N.J. Hodges et al, Mutagenesis 23(4):293-298

Cytogenetic status and oxidative DNA-damage induced by atorvastatin in human peripheral blood lymphocytes: Standard and Fpg-modified comet assay
(2008) G. Gajski et al, Tox. & Appl. Pharma. 231(1):85-93

Cytotoxic and genotoxic potential of drinking water: A comparison between two different concentration methods
(2008) A. Buschini et al, Water Res. 42(1):8-9

Different sensitivities of human colon adenocarcinoma (CaCo-2), astrocytoma (IPDDC-A2) and lymphoblastoid (NCNC) cell lines to microcystin-LR induced reactive oxygen species and DNA damage
(2008) B. Žeguraa et al, Toxicon 52(3):518-525

Discriminative protection against hydroxyl and superoxide anion radicals by quercetin in human leucocytes in vitro
(2008) L.C. Wilms, Tox. in Vitr. 22(2):301-307

Distinct roles of Topoisomerase II isoforms: DNA damage accelerating a, double strand break repair promoting ß
(2008) R. Kumar Mandraju et al, Arch. Bchem. & Bphys. 470(1):27-34

E2F1 Regulates the Base Excision Repair Gene XRCC1 and Promotes DNA Repair
(2008) D. Chen et al, J. Biol. Chem. 283(22):15381-15389

Effect of lead on proliferation and neural differentiation of mouse bone marrow-mesenchymal stem cells
(2008) S. Kermani et al, Tox. in Vitro 22(4):995-1001

Enantioselective cytotoxicity of the insecticide bifenthrin on a human amnion epithelial (FL) cell line
(2008) H. Liu, Toxicology 253(1-3):89-96

Epigenetic Reprogramming of IGF-1 and Leptin Genes by Serum Deprivation in Multipotential Mesenchymal Stromal Cells
(2008) C. Sanchez et al, Stem cells [Epub ahead of print]

Evaluation of chlorite and chlorate genotoxicity using plant bioassays and in vitro DNA damage tests
(2008) D. Feretti et al, Water Res. 42(15):4075-4082

Evaluation of Radioprotective Effects of Propolis and Quercetin on Human White Blood Cells in Vitro
(2008) V. Benkovic et al, Biol. Pharm. Bull. 31(1):1778

Evaluation of the Radioprotective Effects of Propolis and Flavonoids in Gamma-Irradiated Mice: The Alkaline Comet Assay Study
(2008) V. Benkovic et al, Biol. Pharm. Bull. 31(1):167-172

Evaluation of Time Dependence and Interindividual Differences in Benzo[a]pyrene-Mediated CYP1A1 Induction and Genotoxicity in Porcine Urinary Bladder Cell Cultures
(2008) S. Plottner et al, J. Tox. & Env. Health 71(13-14):969-975

Genome Damage in Oropharyngeal Cancer Patients Treated by Radiotherapy
(2008) M. Gamulin, Croat. Med. J. 49(4):515–527

Genome damage in oropharyngeal cancer patients treated by radiotherapy
(2008) M. Gamulin et al, Croat Med. J. 49(4):515-27

Genotoxic damage in pathology anatomy laboratory workers exposed to formaldehyde
(2008) S. Costa, Toxicology 252(1-3):40-48

Genotoxic effect of bile acids on human normal and tumour colon cells and protection by dietary antioxidants and butyrate
(2008) P. Rosignoli et al, Euro. J. Nut. 47(6):301-309

Genotoxic potential of bee venom (Apis Mellifera) on human peripheral blood lymphocytes in vitro using single cell gel electrophoresis assay
(2008) G. Gajski & V. Garaj-Vrhovac, J. Env. Sci. & Health 43(11):1279-1287

Genotoxic pressure of vineyard pesticides in fish: Field and mesocosm surveys
(2008) S. Bony et al, Aqua. Tox. 89(3):197-203

Genotoxicity in primary human peripheral lymphocytes after exposure to radiopacifiers in vitro
(2008) M. Gobbo Braz, J. Mat. Sci. 19(2):601-605

Genotoxicity of low dose N-nitroso propoxur to human gastric cells
(2008) H.H. Kuo et al, Food & Chem. Tox. 46(5):1619-1626

Genotoxicity of Tryptophol in a Battery of Short-Term Assays on Human White Blood Cells in vitro
(2008) I. Kosalec, bas. & Clin. Pharma. Tox. 102(5):443-452

Induction of specific cell responses to a Ca3SiO5-based posterior restorative material
(2008) P. Laurent et al, Dent. Mat. 24(11):1486-1494

Intervention with cloudy apple juice results in altered biological activities of ileostomy samples collected from individual volunteers
(2008) S. Veeriah et al, Euro J. Nutr. 47(5):226-234

l-Carnitine enhances resistance to oxidative stress by reducing DNA damage in Ataxia telangiectasia cells
(2008) A. Berni et al, Mut. Res. 650(2):165-174

Levels of DNA damage in blood leukocyte samples from non-diabetic and diabetic female rats and their fetuses exposed to air or cigarette smoke
(2008) P. Helena Ortiz Lima et al, Mut. Res. 653(1):44-49

Long-Term Functional Stability of Human HepaRG Hepatocytes and Use for Chronic Toxicity and Genotoxicity Studies
(2008) R. Jossé et al, Drug Met. & Disp. 36(1):1111-1118

Low sensitivity of the comet assay to detect acetaldehyde-induced genotoxicity
(2008) G. Speit et al, Mut. Res. 657(2):93-97

Nanoparticle-driven DNA damage mimics irradiation-related carcinogenesis pathways
(2008) R.M. Mroz et al, Eur. Respir. J. 31(1):241-251

No Differences in DNA Damage and Antioxidant Capacity Between Intervention Groups of Healthy, Nonsmoking Men Receiving 2, 5, or 8 Servings/Day of Vegetables and Fruit
(2008) K. Briviba et al, Nut. & Cancer 60(2):164-170

Obesity-related promotion of aberrant crypt foci in DMH-treated obese Zucker rats correlates with dyslipidemia rather than hyperinsulinemia
(2008) T.C.L. Koch et al, Euro J. Nutr. 47(3):161-170

Oxidative DNA damage and total antioxidant status in rats during experimental gram-negative sepsis
(2008) C. Kaymak et al, Human & Exp. Tox. 27(6):485-491

Plx1 is required for chromosomal DNA replication under stressful conditions
(2008) K. Trenz et al, EMBO Journal 282(2):831-835

Polymorphisms in metabolic GSTP1 and DNA-repair XRCC1 genes with an increased risk of DNA damage in pesticide-exposed fruit growers
(2008) R. Wong et al, Mut. Res. 654(2):168-175

Reactive oxygen species from the uncoupling of human cytochrome P450 1B1 may contribute to the carcinogenicity of dioxin-like polychlorinated biphenyls
(2008) R.M. Green et al, Mutagenesis 23(6):457-463

Recommendations for design of the rat comet assay
(2008) C.C. Smith et al, Mutagenesis 23(3):233-40

Relationship among Oxidative DNA Damage, Gastric Mucosal Density and the Relevance of cagA , vacA and iceA Genotypes of Helicobacter pylori
(2008) M.S.P. Ladeira et al, Dig. Dis. & Sci. 51(1):248-255

Repair of Sparfloxacin-Induced Photochemical DNA Damage In Vivo
(2008) M. Struwe et al, J. Inv. Derma. [Epub ahead of print]

Subcellular distribution of inorganic and organic arsenic compounds in human urothelial cells and human hepatocytes
(2008) E Dopp et al, Drug Metab Dispos. 36(5):971-979

Synthesis and biological activity of stable and potent antitumor agents, aniline nitrogen mustards linked to 9-anilinoacridines via a urea linkage
(2008) N. Kapuriya et al, Bio. & Med. Chem. 16(10):5413-5423

Tenocyclidine treatment in soman-poisoned rats--intriguing results on genotoxicity versus protection.
(2008) M.J. Petek et al, Acta. Biochim. Pol. 55(1):97-105

The effect of inhibited replication on DNA migration in the comet assay in relation to cytotoxicity and clastogenicity
(2008) G. Speit & P. Schütz, Mut. Res. 655(1-2):22-27

The genotoxic potential of glutaraldehyde in mammalian cells in vitro in comparison with formaldehyde
(2008) G. Speit et al, Mut. Res. 649(1-2):146-154

The JmjC Domain Histone Demethylase Ndy1 Regulates Redox Homeostasis and Protects Cells from Oxidative Stress
(2008) C. Polytarchou et al, Mol. Cell. Biol. 28(24):7451-7464

Tomato oleoresin inhibits DNA damage but not diethylnitrosamine-induced rat hepatocarcinogenesis
(2008) C. Scolastici et al, Exp. & Tox. Path. 60(1):59-68

Use of Comet-FISH in the study of DNA damage and repair: Review
(2008) M. Glei et al, Mut. Res. [Epub]

ß-Carotene metabolites enhance inflammation-induced oxidative DNA damage in lung epithelial cells
(2008) Y.G.J. van Helden et al, Free Rad. Biol. & Med. 46(2):299-304


We have had no problems so far, and we are very pleased with the system.

Senior Researcher, Public Health Agency, Finland

We are very satisfied with your software.

Scientist, Cancer Research Institute, Slovakia

We are using the Comet Assay IV a lot (and our use is increasing). It's working very well.

Professor, Children's Research Hospital, USA

Excellent, perfectly straightforward.

Scientist, University, UK

The installation was quick and all the details were described and easily understandable for the technicians.

Head of Department, Global Chemical Company, Germany

I have been using the Comet Assay IV Software. It is great! During my software validation, I realized how much easier and precise it is compared to our old software. It makes analyzing the comet slides more enjoyable

Scientist, Medical Research Centre, USA

Excellent, the training was very helpful and specific to in-house technicalities.

Lecturer, University, UK

So it's working out fine and we're very happy!

Toxicologist, Health Agency, Canada

It's an amazing program! Really useful!

Research Scientist, University, Brazil

I love the software. Keep up the good work over there.

Study Director, Contract Research Organisation, USA

Total change in our lab since you came (with Comet Assay IV of course...)! thanks... :-)

Postgraduate Researcher, University, France

The system was set up last month, and so far it works very well. Both my boss and I are very satisfied with it and like it very much.

Researcher, University Hospital, Germany

I'm really a fan of your system :)

Genetic Toxicologist, Global Pharmaceutical Company

Everything has gone very well with the system and we are very pleased with it.

Professor, University, UK

We have found the Comet Assay IV software very useful and convenient.

Research Scientist, Food Safety Agency, France

We're now using the software and it works great. It has cut our time for comets by at least half.

Post Doctoral Fellow, National Institute of Health, USA

...Everything is fine with the Comet Assay IV system...and I'm pretty happy about this new software with just clicking on the cells instead of drawing a frame around them

Researcher, Cancer Research Organisation, UK

All is going well and the more I use the software the more I love the way it's so simple and intuitive...the comet software is running well and in increasing use.

Scientist, University, UK

I don't have any questions about the software as it has worked quite well for far we are very happy with the software.

Research Scientist, University, Australia

The Comet Assay IV system we already installed and is working perfectly.

Scientist, Environmental Institute, Norway

...and the system is running great.

Medical Researcher, Cancer Research Institute, USA

The current Comet Assay IV system works without any trouble…we are pleased with the system.

Scientist, Chemical Company, Japan

Yes, it is very useful and we are in the process of writing a paper also from the data derived from Comet Assay IV.

Assistant Professor, University, USA

The system is working great.

Senior Research Scientist, Cancer Research Institute, UK

We have installed the software and are using it without any problem. It's really simple! My PhD students are very happy with it.

Professor, University, Spain

...have been using it now for a couple of weeks and it seems to be working great. Very user friendly - I love it as does my intern!

Staff Chemist, Marine Laboratory, USA

We are very happy with everything, it is easy to work with your software.

Senior Scientist, National Health Institute, Portugal

So far, I'm very happy with the system.

Research Lecturer, University, UK

We started using Comet Assay IV and it works well...

Researcher, Nuclear Agency, France

Everything has gone very well with the system and we are very pleased with it.

Scientist, University, UK

The system is up and running. Everybody is impressed with the speed of scoring slides.

Researcher, University, South Africa

Comet Assay IV software is in use. Currently our comet analysis is based on tail moment measurement and I must say that everything works properly.

Researcher, Cancer Research Institute, Slovakia

Thanks for your attention and great service. I am so glad that we have Comet IV.

Study Director, Contract Research Organisation, USA

Just to let you know we have started the validation study for Comet Assay IV and it has been going very well! We ran a comet assay last week and scored the slides with no problems at all. I am very pleased with the system and it will certainly make a difference when we run some comet studies this year!

Study Director, Food & Household Products Company, UK

...we are "happy users" of the software

Senior Researcher, Risk Assessment Institute, Germany

We are really very happy with the comet analysis system.

Research Scientist, University, Austria

We have just recently utilized the system and are extremely pleased.

Assistant Professor, University, USA

Perfect and very clear installation and training.

Study Director, Contract Research Organisation, Europe

It is actually working great.

Professor, University, USA

It is, indeed, much quicker than the old image analysis system that we had. We are very pleased with it.

Senior Researcher, Public Health Agency, Finland

We are impressed indeed! It has saved us quite a lot of time and pains.

Director, Toxicology Research Centre, Spain