Papers from 2007

Below are a number of refereed papers from 2007 in which Perceptive Instruments software is used for the comet assay. Applications of the systems include potentially harmful and beneficial dietary factors; biomonitoring; molecular epidemiology; photobiology; mechanistic studies, clinical and dentistry.

8-Oxoguanine DNA Glycosylase and MutY Homolog Are Involved in the Incision of Arsenite-Induced DNA Adducts
(2007) Y. Pu et al, Tox. Sci. 95(2):376-382

Absence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats
(2007) A. de Lima Leite et al, Human & Expt. Tox. 26(5):435-440

An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans
(2007) I. H. Ismail et al, Nuc. Acids Res. 35(5):e36

Antigenotoxicity and antimutagenicity of lycopene in HepG2 cell line evaluated by the comet assay and micronucleus test
(2007) C.Scolasti et al, Tox. in Vitro 22(2):510-514

Biocompatibility of gutta-percha solvents using in vitro mammalian test-system
(2007) D. Ribeiro et al, Oral Surg. 103(5):e106-e109

Blood mononucleocytes are sensitive to the DNA damaging effects of iron overload—In vitro and ex vivo results with human and rat cells
(2007) E. Park et al, Mut. Res. 619(1-2):59-67

Characterization of the genotoxic potential of formaldehyde in V79 cells
(2007) G. Speit et al, Mutagenesis 22(6):387-394

Chicken nucleated blood cells as a cellular model for genotoxicity testing using the comet assay
(2007) M. Sokolovic et al, Food & Chem. Tox. 45(11):2165-2170

Comparative evaluation of acetylcholinesterase status and genome damage in blood cells of industrial workers exposed to carbofuran
(2007) D. Zeljezic et al, Food and Chem. Tox. 45(12):2488-2498

Dietary synbiotics reduce cancer risk factors in polypectomized and colon cancer patients
(2007) J. Rafter et al, Amer. J. Clin. Nutr. 85(2):488-496

Differential response related to genotoxicity between eggplant (Solanum melanogena) skin aqueous extract and its main purified anthocyanin (delphinidin) in vivo
(2007) L. Azevedo et al, Food & Chem. Tox. 45(5):852-858

DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi
(2007) D. Ribeiro et al, Expt. Parasitology. 116(3):296-301

DNA damage in multiple organs after exposure to chlorhexidine in Wistar rats
(2007) T. Grassi et al, Int. J. Hygiene & Env. Health 210(2):163-167

DNA damage, acetylcholinesterase activity and lysosomal stability in native and transplanted mussels (Mytilus edulis) in areas close to coastal chemical dumping sites in Denmark
(2007) J. Rank et al, Aqua. Tox. 84(1):50-61

DNA-damaging ability of isoprene and isoprene mono-epoxide (EPOX I) in human cells evaluated with the comet assay
(2007) R. Fabiani et al, Mut. Res. 629(1):7-13

Essential Roles for Fe65, Alzheimer Amyloid Precursor-binding Protein, in the Cellular Response to DNA Damage
(2007) G. Minopoli et al, J. Biol. Chem. 282(2):831-835

Evaluation of level of DNA damage in blood leukocytes of non-diabetic and diabetic rat exposed to cigarette smoke
(2007) P. H. Ortiz Lima et al, Mut. Res. 628(2):117-122

Fecal Water as a Non-Invasive Biomarker in Nutritional Intervention: Comparison of Preparation Methods and Refinement of Different Endpoints
(2007) A. Klinder et al, Nut. & Cancer 57(2):158-167

Fumonisin B1: Oxidative status and DNA damage in rats
(2007) A. Domijan et al, Toxicology 232(3):163-169

Genetic damage in human peripheral lymphocytes exposed to antimicrobial endodontic agents
(2007) G. N. Da Silva et al, Oral Surg. 104(2):e58-e61

Genotoxic effects in the Eastern mudminnow (Umbra pygmaea L.) after exposure to Rhine water, as assessed by use of the SCE and Comet assays: A comparison between 1978 and 2005
(2007) G. M. Alink et al, Mut. Res. 631(2):93-100

Genotoxic effects induced by formaldehyde in human blood and implications for the interpretation of biomonitoring studies
(2007) O. Schmid and G. Speit, Mutagenesis 22(1):69-74

Genotoxic effects of exposure to radiofrequency electromagnetic fields (RF-EMF) in cultured mammalian cells are not independently reproducible
(2007) G. Speit et al, Mut. Res. 622(1-2):42-47

Genotoxic effects of particles of surgical cobalt chrome alloy on human cells of different age in vitro
(2007) I. Papageorgiou et al, Mut. Res. 619(1-2):45-58

Genotoxicity of Corrosion Eluates Obtained From Endosseous Implants
(2007) D. Ribeiro et al, Implant Dent. 16(1):101-109

GSTM1 and GSTT1 polymorphism influences protection against induced oxidative DNA damage by quercetin and ascorbic acid in human lymphocytes in vitro
(2007) L. C. Wilms et al, Food & Chem. Tox. 45(12):2592-2596

Human gastric cells resistant to (-)-epigallocatechin gallate show cross-resistance to several environmental pollutants
(2007) S.B. Horng et al, Food & Chem. Tox. 45(11):2171-2178

Impact of multiple genetic polymorphisms on effects of a 4-week blueberry juice intervention on ex vivo induced lymphocytic DNA damage in human volunteers
(2007) L. C. Wilms et al, Carcinogenesis 28(8):1800-1806

In vitro cytotoxicity and genotoxicity of chlorinated drinking waters sampled along the distribution system of two municipal networks
(2007) L. Marabini et al, Mut. Res. 634(1-2):1-13

Influence of endogenous and synthetic female sex hormones on human blood cells in vitro studied with comet assay
(2007) M. Gobbo Braz and D. M. Fávero Salvadori, Tox. in Vitro 21(5):972-976

Inhibition of the mitochondrial respiratory chain function abrogates quartz induced DNA damage in lung epithelial cells
(2007) H. Li et al, Mut. Res. 617(1-2):46-57

Irinotecan Toxicity to Human Blood Cells in vitro: Relationship between Various Biomarkers
(2007) N. Kopjar et al, Bas. Clin. Pharma. & Tox. 100(6):403-413

Lycopene activity against chemically induced DNA damage in Chinese hamster ovary cells
(2007) C. Scolastici et al, Tox. in Vitro 21(5):840-845

Poly(ADP-Ribose) Polymerase 1 Accelerates Single-Strand Break Repair in Concert with Poly(ADP-Ribose) Glycohydrolase
(2007) A. E. O. Fisher et al, Mol. Cell. Biol. 27(11):5597-5605

Primary DNA damage and genetic polymorphisms for CYP1A1, EPHX and GSTM1 in workers at a graphite electrode manufacturing plant
(2007) M. Moretti et al, BMC Pub. Health 7:270

TDP1 facilitates chromosomal single-strand break repair in neurons and is neuroprotective in vivo
(2007) S. Katyal et al, EMBO J. 26:4720-4731

TDP1 facilitates repair of ionizing radiation-induced DNA single-strand breaks
(2007) S. F. El-Khamisy et al, DNA Repair 6(10):1485-1495

The effect of nano- and micron-sized particles of cobalt–chromium alloy on human fibroblasts in vitro
(2007) I. Papageorgiou et al, Biomaterials 28(19):2946-2958

The environmental carcinogen 3-nitrobenzanthrone and its main metabolite 3-aminobenzanthrone enhance formation of reactive oxygen intermediates in human A549 lung epithelial cells
(2007) T. Hansen et al, Tox. & App. Pharma. 221(2):222-234

The quercetin paradox
(2007) A.W. Boots et al, Tox & Appl. Pharma. 222(1):89-96

Toxicological potential of 2-alkylcyclobutanones – specific radiolytic products in irradiated fat-containing food – in bacteria and human cell lines
(2007) A. Hartwig et al, Food & Chem. Tox. 45(12):2581-2591


We started using Comet Assay IV and it works well...

Researcher, Nuclear Agency, France

Thanks for your attention and great service. I am so glad that we have Comet IV.

Study Director, Contract Research Organisation, USA

Yes, it is very useful and we are in the process of writing a paper also from the data derived from Comet Assay IV.

Assistant Professor, University, USA

So it's working out fine and we're very happy!

Toxicologist, Health Agency, Canada

It is, indeed, much quicker than the old image analysis system that we had. We are very pleased with it.

Senior Researcher, Public Health Agency, Finland

We are impressed indeed! It has saved us quite a lot of time and pains.

Director, Toxicology Research Centre, Spain

We have found the Comet Assay IV software very useful and convenient.

Research Scientist, Food Safety Agency, France

We have had no problems so far, and we are very pleased with the system.

Senior Researcher, Public Health Agency, Finland

The Comet Assay IV system we already installed and is working perfectly.

Scientist, Environmental Institute, Norway

...we are "happy users" of the software

Senior Researcher, Risk Assessment Institute, Germany

We are using the Comet Assay IV a lot (and our use is increasing). It's working very well.

Professor, Children's Research Hospital, USA

Excellent, perfectly straightforward.

Scientist, University, UK

Perfect and very clear installation and training!

Researcher, Contract Testing Laboratory, Europe

We're now using the software and it works great. It has cut our time for comets by at least half.

Post Doctoral Fellow, National Institute of Health, USA

Excellent, the training was very helpful and specific to in-house technicalities.

Lecturer, University, UK

Comet Assay IV software is in use. Currently our comet analysis is based on tail moment measurement and I must say that everything works properly.

Researcher, Cancer Research Institute, Slovakia

The installation was quick and all the details were described and easily understandable for the technicians.

Head of Department, Global Chemical Company, Germany

Perfect and very clear installation and training.

Study Director, Contract Research Organisation, Europe

I love the software. Keep up the good work over there.

Study Director, Contract Research Organisation, USA

We are really very happy with the comet analysis system.

Research Scientist, University, Austria

Everything has gone very well with the system and we are very pleased with it.

Scientist, University, UK

The system was set up last month, and so far it works very well. Both my boss and I are very satisfied with it and like it very much.

Researcher, University Hospital, Germany

We have just recently utilized the system and are extremely pleased.

Assistant Professor, University, USA

The system is up and running. Everybody is impressed with the speed of scoring slides.

Researcher, University, South Africa

...have been using it now for a couple of weeks and it seems to be working great. Very user friendly - I love it as does my intern!

Staff Chemist, Marine Laboratory, USA

I have been using the Comet Assay IV Software. It is great! During my software validation, I realized how much easier and precise it is compared to our old software. It makes analyzing the comet slides more enjoyable

Scientist, Medical Research Centre, USA

I'm really a fan of your system :)

Genetic Toxicologist, Global Pharmaceutical Company

Total change in our lab since you came (with Comet Assay IV of course...)! thanks... :-)

Postgraduate Researcher, University, France

The system is working great.

Senior Research Scientist, Cancer Research Institute, UK

...and the system is running great.

Medical Researcher, Cancer Research Institute, USA

The current Comet Assay IV system works without any trouble…we are pleased with the system.

Scientist, Chemical Company, Japan

We have installed the software and are using it without any problem. It's really simple! My PhD students are very happy with it.

Professor, University, Spain

All is going well and the more I use the software the more I love the way it's so simple and intuitive...the comet software is running well and in increasing use.

Scientist, University, UK

It's an amazing program! Really useful!

Research Scientist, University, Brazil

We are very happy with everything, it is easy to work with your software.

Senior Scientist, National Health Institute, Portugal

Just to let you know we have started the validation study for Comet Assay IV and it has been going very well! We ran a comet assay last week and scored the slides with no problems at all. I am very pleased with the system and it will certainly make a difference when we run some comet studies this year!

Study Director, Food & Household Products Company, UK

I don't have any questions about the software as it has worked quite well for far we are very happy with the software.

Research Scientist, University, Australia

Everything has gone very well with the system and we are very pleased with it.

Professor, University, UK

So far, I'm very happy with the system.

Research Lecturer, University, UK

...Everything is fine with the Comet Assay IV system...and I'm pretty happy about this new software with just clicking on the cells instead of drawing a frame around them

Researcher, Cancer Research Organisation, UK

We are very satisfied with your software.

Scientist, Cancer Research Institute, Slovakia