Papers from 2010

A multibiomarker approach in Coris julis living in a natural environment
(2010) S. Fasulo et al, Ecototoxicol. and Environ. Safety

An ECVAG dagger trial on assessment of oxidative damage to DNA measured by the comet assay
(2010) C Johansson et al, Mutagenesis 25(2):125-132

Anomalous genotoxic responses induced in mouse lymphoma L5178Y cells by potassium bromate
(2010) C. Priestley et al, Toxicology 267(1-3):45-53

Antimicrobial activity of commercial zeolite A on Acinetobacter junii and Saccharomyces cerevisiae
(2010) J. Hrenovic et al, Journal of Hazardous Materials 183(1-3): 655-663

Are endogenous sex hormones related to DNA damage in paradoxically sleep-deprived female rats?
(2010) M.L. Andersen et al, Hormones and Behaviour 57(2):216-221

Assessing the DNA methylation status of single cells with the comet assay
(2010) J Wentzel et al, Analytical Biochem. 400(2):190-194

Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay
(2010) V Kasuba et al, Arch Ind Hyg Toxicol. 61(1): 1-9

Assessment of cytogenetic damage and oxidative stress in personnel occupationally exposed to the pulsed microwave radiation of marine radar equipment
(2010) V. Garaj-Vrhovac et al, Int. J. Hyg. Environ. Health (e-pub)

Assessment of DNA Damage by the Comet Assay: Comparative Evaluation of Methods for Preparation of Single-Cell Suspensions from Multiple Tissues of Rats
(2010) M Manjanatha et al, Abstracts from the 8th International Comet Assay Workshop held at the University of Perugia, Perugia, Italy; 27-30 August 2009 25(1): 12

Assessment of okadaic acid effects on cytotoxicity, DNA damage and DNA repair in human cells
(2010) V. Valdiglesiasa et al, Mut Res. 689(1):74-79

Assessment of the genotoxic potential of indirect chemical mutagens in HepaRG cells by the comet and the cytokinesis-block micronucleus assays
(2010) L Le Hegarat et al, Mutagenesis (e-pub)

Beauvericin and ochratoxin A genotoxicity evaluated using the alkaline comet assay: single and combined genotoxic action
(2010) M.Š. Klarić et al, Archives Toxicol. 84(8):641-650

Berberine and a Berberis lycium extract inactivate Cdc25A and induce α-tubulin acetylation that correlate with HL-60 cell cycle inhibition and apoptosis
(2010) M. Khan et al., Mutat Res. 683(1-2):123-130

BLM Deficiency Is Not Associated with Sensitivity to Hydroxyurea-Induced Replication Stress
(2010) K. Bennani-Belhaj et al, J. Nucleic Acids (e-pub)

Bolus ingestion of white and green tea increases the concentration of several flavan-3-ols in plasma, but does not affect markers of oxidative stress in healthy non-smokers.
(2010) N. Muller et al, Mol. Nutr. Food Res. [e-pub]

Brain DNA damage and 70-kDa heat shock protein expression in CD1 mice exposed to extremely low frequency magnetic fields
(2010) G. Mariucci et al, Int. J. Rad. Biol. 86(8):701-710

Cellular uptake, subcellular distribution and toxicity of arsenic compounds in methylating and non-methylating cells
(2010) E Dopp et al, Environm. Res. (in press)

Combined effects of 872 MHz radiofrequency radiation and ferrous chloride on reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells
(2010) J. Luukkonen et al, Bioelectromagnetics 31(6):417–424

Comparative Growth Performance of Mixed-Sex and Monosex Nile Tilapia Population in Freshwater Cage Culture System under Indian Perspective
(2010) SB Chakraborty & S Banerjee, Int J Biol. 2(1):44-50

Correlation between folate and vitamin B12 and markers of DNA stability in healthy men: preliminary results.
(2010) M Milić et al, Acta Biochim [e-pub]

Coupling cytotoxicity biomarkers with DNA damage assessment in TK6 human lymphoblast cells
(2010) J. Shi et al, Mut. Res. 696(2):167-178

Cyto- and genotoxicological assessment and functional characterization of N-vinyl-2-pyrrolidone–acrylic acid-based copolymeric hydrogels with potential for future use in wound healing applications
(2010) D Kirf et al, Biomed Mater. 5(3)

Cytogenetic Follow-Up in Testicular Seminoma Patients Exposed to Adjuvant Radiotherapy
(2010) M. Gamulin et al, Coll. Antropol. 34(2): 455–465

Cytotoxicity and Regenerative Proliferation as the Mode of Action for Diuron-Induced Urothelial Carcinogenesis in the Rat
(2010) M.S. da Rocha et al, Toxicol. Sci. 113(1):37-44

Differential effects of nitro-PAHs and amino-PAHs on cytokine and chemokine responses in human bronchial epithelial BEAS-2B cells
(2010) J. Øvrevik et al, Toxicol. Applied Pharmacol. 242(3):270-280

Differential toxicity of heterocyclic aromatic amines and their mixture in metabolically competent HepaRG cells
(2010) J Dumont et al, Tox App Pharma. (e-pub)

Diminished carcinogen detoxification is a novel mechanism for hypoxia inducible factor 1 (HIF-1) mediated genetic instability
(2010) M A Shults et al, J Bio Chem. (e-pub)

DNA and cytogenetic damage in white blood cells of postmenopausal breast cancer patients treated with radiotherapy
(2010) M. Gamulin et al, J. Environ. Sci. and Health, Part A, 45(3): 292 - 304

DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test
(2010) G. Brozovic et al, J Appl Genet 51(1):79–86

DNA Damage in Workers Occupationally Exposed to Lead, Using Comet Assay
(2010) P.Manikantan et al, Int J Biology 2(1):103-110

DNA damage, glutathione, and total antioxidant capacity in anesthesia nurses
(2010) S. Isdez et al, Arch Environ Occup Health. 65(4):211-7

DNA strand breakage and lipid peroxidation after exposure to welding fumes in vivo
(2010) C.H. Chuang et al., Mutagenesis 25(1):71-76

Does potassium sorbate induce genotoxic or mutagenic effects in lymphocytes?
(2010) S. Mamur et al, Toxicol. in Vitro 24(3): 790-794

Effects of lycopene, synbiotic and their association on early biomarkers of rat colon carcinogenesis
(2010) M.C. Dias et al, Food and Chem. Toxicol. 48(3):772-780

Estimation of DNA Integrity in Blood Cells of Eastern Mosquitofish (Gambusia holbrooki) Inhabiting an Aluminium-Polluted Water Environment: an Alkaline Comet Assay Study
(2010) I Ternjej et al, Archives of Environmental Contamination and Toxicology 59(2):182-93

Evaluation of cytotoxicity and oxidative DNA damaging effects of di(2-ethylhexyl)-phthalate (DEHP) and mono(2-ethylhexyl)-phthalate (MEHP) on MA-10 Leydig cells and protection by selenium
(2010) P. Erkekoglu et al, Toxicol. Applied Pharmacol. 248(1):52-62

Exposure of human nasal epithelial cells to formaldehyde does not lead to DNA damage in lymphocytes after co-cultivation
(2010) S Neuss et al, Mutagenesis (e-pub)

Expression of glutathione S-transferase M2 in stage I/II non-small cell lung cancer and alleviation of DNA damage exposure to benzo[a]pyrene
(2010) S. Tang et al, Toxicology Letters 192(3):316-323

Free radical-scavenging activity and DNA damaging potential of auxins IAA and 2-methyl-IAA evaluated in human neutrophils by the alkaline comet assay
(2010) S. Branka et al, J Biochem Mol Toxicol. 24(3):165–173

Ganglioside GT1b protects human spermatozoa from hydrogen peroxide-induced DNA and membrane damage
(2010) M Gavella et al, Int. J. Androl. 33(3):536-544

Gene expression changes in primary human nasal epithelial cells exposed to formaldehyde in vitro
(2010) S. Neuss et al, Toxicol. Letters 198(2):289-295

Gene toxicity studies on titanium dioxide and zinc oxide nanomaterials used for UV-protection in cosmetic formulations
(2010) R. Landsiedel et al, Nanotox. 4(4 ):364-381

Genistein and β-carotene enhance the growth-inhibitory effect of trichostatin A in A549 cells
(2010) R Shiau et al, Euro J Nut. 49(1):19-25

Genomic instability in a healthy elderly population: a pilot study of possible cytogenetic markers related to ageing
(2010) M. Mladinic et al, Mutagenesis [e-pub]

Genomic instability in a healthy elderly population: a pilot study of possible cytogenetic markers related to ageing
(2010) M. Mladinic et al, Mutagenesis 25(5):455-462

Genotoxic effects of neutrophils and hypochlorous acid
(2010) N Gungor et al, Mutagenesis 25(2):149-154

Genotoxicity assessment in the amphipod Gammarus fossarum by use of the alkaline Comet assay
(2010) E. Lacaze et al, Mut. Res. 700(1-2):32-38

Genotoxicity assessment of two vineyard pesticides in zebrafish
(2010) S. Bony et al, Int. J. Env. Analyt. Chem 90(3):421-428

Genotoxicity of anti-tumor necrosis factor therapy in patients with juvenile idiopathic arthritis.
(2010) E. Demirkaya et al, Arthritis Care Res. 62(1):73-7

Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells
(2010) S.H. Inayat-Hussain et al, Toxicol. Letters 193(1):108-114

Hydrogen peroxide mediates EGCG-induced antioxidant protection in human keratinocytes
(2010) L. Elbling et al, Free Radical Biology and Medicine 49(9): 1444-1452

Impact of paper filtered coffee on oxidative DNA-damage: Results of a clinical trial
(2010) M. Misik et al, Mut. Res. 692(1-2): 42-48

In vitro effect of the antimalarial drug proguanil hydrochloride on viability and DNA damage in human peripheral blood lymphocytes
(2010) G Gajski et al, Enviro. Tox. & Pharmacol. (in press)

In vitro evaluation of baseline and induced DNA damage in human sperm exposed to benzo[a]pyrene or its metabolite benzo[a]pyrene-7,8-diol-9,10-epoxide, using the comet assay
(2010) V. Sipinen et al, Mutagenesis [e-pub]

In vitro evaluation of SiC nanoparticles impact on A549 pulmonary cells: Cyto-, genotoxicity and oxidative stress
(2010) S. Barillet et al, Toxicology Letters 198(3): 324-330

In vitro investigations of glycidamide-induced DNA lesions in mouse male germ cells and in mouse and human lymphocytes
(2010) S H Hansen et al, Mut Res Gen Tox. 696(1):55-61

In vivo bioassay to detect irinotecan-stabilized DNA/topoisomerase I complexes in rats
(2010) S W Barth et al, Biotech J. 5(3):321-327

Increasing fish consumption does not affect genotoxicity markers in the colon in an intervention study
(2010) G.K. Pott et al, Carcinogenesis 31(6): 1087-1091

Influence of diet on oxidative DNA damage, uracil misincorporation and DNA repair capability
(2010) R.P. Prado et al, Mutagenesis 25(5): 483-487

Inhalation of formaldehyde does not induce genotoxic effects in broncho-alveolar lavage (BAL) cells of rats
(2010) S. Neuss et al, Mut Res. 695(1-2):61-68

Instant coffee with high chlorogenic acid levels protects humans against oxidative damage of macromolecules
(2010) C Hoelzl et al, Mol. Nut. & Food Res. (e-pub)

Low air levels of benzene: Correlation between biomarkers of exposure and genotoxic effects
(2010) M.E. Fracasso et al, Toxicology Letters 192(1):22-28

Marginal zinc deficiency increases oxidative DNA damage in the prostate after chronic exercise
(2010) Y. Song et al., Free Rad Biol Med. 48(1):82-88

Mutagenic response of 2.45GHz radiation exposure on rat brain
(2010) K K Kesari et al, Int J Rad Biol. 86(4):334-343

No increases in biomarkers of genetic damage or pathological changes in heart and brain tissues in male rats administered methylphenidate hydrochloride (Ritalin) for 28 days
(2010) K.L. Wit et al, Environ. Molecular Mutagenesis 51(1):80–88

Nuclear phosphoinositide 3-kinase β controls double-strand break DNA repair
(2010) A Kumar et al, PNAS 107(16):7491-7496

Ofloxacin induces oxidative damage to joint chondrocytes of juvenile rabbits: Excessive production of reactive oxygen species, lipid peroxidation and DNA damage
(2010) Q. Li et al., Euro J Pharmacol. 626(2-3):146-153

Oxidative stress and DNA damage responses in rat and mouse lung to inhaled carbon nanoparticles
(2010) A. Wessels et al, Nanotox [e-pub]

Partial hydrolysis enhances the inhibitory effects of konjac glucomannan from Amorphophallus konjac C. Koch on DNA damage induced by fecal water in Caco-2 cells
(2010) Shu-Lan Yeh et al, Food Chem. 119(2):614-618

PERK promotes cancer cell proliferation and tumor growth by limiting oxidative DNA damage
(2010) E Bobrovnikova-Marjon et al, Oncogene (e-pub)

Polyphenolic Apple Extracts: Effects of Raw Material and Production Method on Antioxidant Effectiveness and Reduction of DNA Damage in Caco-2 Cells
(2010) P. Bellion et al, J. Agricult. Food Chem 58(11):6636-6642

Polyphenolic Compounds from Salvia Species Protect Cellular DNA from Oxidation and Stimulate DNA Repair in Cultured Human Cells
(2010) A. Ramos et al, J. Agricultural Food Chem. 58(12):7465-7471

Prevention of oxidative DNA damage in inner organs and lymphocytes of rats by green tea extract
(2010) N. Kager et al, Euro. J. Nutrition 49(4):1436-6207

Protective effect of selenium supplementation on the genotoxicity of di (2-ethylhexyl) phthalate and mono (2-ethylhexyl) phthalate treatment in LNCaP cells
(2010) P Erkekoglua et al, Free Rad Bio Med. (e-pub)

Protective effects of Ursolic acid and Luteolin against oxidative DNA damage include enhancement of DNA repair in Caco-2 cells
(2010) A.A. Ramos et al, Mut. Res. 692(1-2):6-11

Rapid method for determination of DNA repair capacity in human peripheral blood lymphocytes amongst smokers
(2010) R El-Zein et al, BMC Cancer 10:439

Rejoining of gamma-ray-induced DNA damage in Cryptosporidium parvum measured by the comet assay
(2010) S Lee et al, Exp. Para. (e-pub)

Relation between DNA repair, apoptosis and chromosomal aberrations in presence of pifithrin-α, an inhibitor of p53
(2010) R Meschini et al, Mut Res. (e-pub)

Relationship between chromatin structure, DNA damage and repair following X-irradiation of human lymphocytes
(2010) P Mosesso et al, Mut Res. (e-pub)

Sensitization to Radiation and Alkylating Agents by Inhibitors of Poly(ADP-ribose) Polymerase Is Enhanced in Cells Deficient in DNA Double-Strand Break Repair
(2010) D Loser et al, Mol. Cancer Therap [e-pub]

Single exposure to cocaine or ecstasy induces DNA damage in brain and other organs of mice.
(2010) T. Alvarenga et al, Addict Biol. 15(1):96-9

Spectrophotometric analysis of flavonoid-DNA interactions and DNA damaging/protecting and cytotoxic potential of flavonoids in human peripheral blood lymphocytes
(2010) G Rusak et al, Chemico-Biol. Interact. (in press)

Spi-1/PU.1 Oncogene Accelerates DNA Replication Fork Elongation and Promotes Genetic Instability in the Absence of DNA Breakage
(2010) P. Rimmele et al, Cancer Res (70):6757-6766

(2010) P Alves de Lima et al, J. Food Biochem. 34(1):206-228

Subcellular Distribution of a Fluorescence-Labeled Combi-Molecule Designed to Block Epidermal Growth Factor Receptor Tyrosine Kinase and Damage DNA with a Green Fluorescent Species
(2010) M I Todorova et al, Mol Cancer Ther. (e-pub)

Synthesis and Uptake of Fluorescence-Labeled Combi-molecules by P-Glycoprotein-Proficient and -Deficient Uterine Sarcoma Cells MES-SA and MES-SA/DX5
(2010) A Larroque-Lombard et al, J. Med. Chem. 53(5):2104-2113

The application of 3D micropatterning of agarose substrate for cell culture and in situ comet assays
(2010) E Mercey et al, Biomaterials 31(12):3156-3165

The human lung cell line A549 does not develop adaptive protection against the DNA-damaging action of formaldehyde.
(2010) G. Speit et al, Environ Mol Mutagen 51(2):130-7

The influence of thermal desorption on genotoxicity of multipolluted soil
(2010) M Bonnard et al, Ecotoxicol Env Safety 73(5):955-960

The role of oxidative DNA damage, DNA repair, GSTM1, SOD2 and OGG1 polymorphisms in individual susceptibility to Barrett’s esophagus
(2010) E Kadioglu et al, Toxicol Ind Health. (e-pub)

Thresholds for indirect DNA damage across cellular barriers for orthopaedic biomaterials.
(2010) M Parry et al, Biomaterials [e-pub]

Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation
(2010) S Shaposhnikov et al, Tox. Let. (e-pub)

Using natural dietary sources of antioxidants to protect against ultraviolet and visible radiation-induced DNA damage: An investigation of human green tea ingestion
(2010) HM de la Roche et al, J Photochem Photobiol. (e-pub)

Validation of a Simple In Vitro Comet Assay Method Using CHL Cells
(2010) A. Kimura et al, Genes and Environment 32(3):61-65

Vitamin C prevents DNA damage induced by renovascular hypertension in multiple organs of Wistar rats
(2010) EE Nishy et al, Hum Exp Toxicol. (e-pub)

Xanthohumol, a prenylated flavonoid contained in beer, prevents the induction of preneoplastic lesions and DNA damage in liver and colon induced by the heterocyclic aromatic amine amino-3-methyl-imidazo[4,5-f]quinoline (IQ)
(2010) F Ferk et al, Mut. Res. (in press)

XPC Silencing Sensitizes Glioma Cells to Arsenic Trioxide via Increased Oxidative Damage
(2010) Shin-Yi Liu et al, Toxicol Sci. (e-pub)


We have found the Comet Assay IV software very useful and convenient.

Research Scientist, Food Safety Agency, France

...we are "happy users" of the software

Senior Researcher, Risk Assessment Institute, Germany

We are really very happy with the comet analysis system.

Research Scientist, University, Austria

Yes, it is very useful and we are in the process of writing a paper also from the data derived from Comet Assay IV.

Assistant Professor, University, USA

Everything has gone very well with the system and we are very pleased with it.

Professor, University, UK

Just to let you know we have started the validation study for Comet Assay IV and it has been going very well! We ran a comet assay last week and scored the slides with no problems at all. I am very pleased with the system and it will certainly make a difference when we run some comet studies this year!

Study Director, Food & Household Products Company, UK

We are very happy with everything, it is easy to work with your software.

Senior Scientist, National Health Institute, Portugal

The installation was quick and all the details were described and easily understandable for the technicians.

Head of Department, Global Chemical Company, Germany

We are impressed indeed! It has saved us quite a lot of time and pains.

Director, Toxicology Research Centre, Spain

So it's working out fine and we're very happy!

Toxicologist, Health Agency, Canada

The system is up and running. Everybody is impressed with the speed of scoring slides.

Researcher, University, South Africa

It is actually working great.

Professor, University, USA

The system was set up last month, and so far it works very well. Both my boss and I are very satisfied with it and like it very much.

Researcher, University Hospital, Germany

We have had no problems so far, and we are very pleased with the system.

Senior Researcher, Public Health Agency, Finland

Comet Assay IV software is in use. Currently our comet analysis is based on tail moment measurement and I must say that everything works properly.

Researcher, Cancer Research Institute, Slovakia

Total change in our lab since you came (with Comet Assay IV of course...)! thanks... :-)

Postgraduate Researcher, University, France

The Comet Assay IV system we already installed and is working perfectly.

Scientist, Environmental Institute, Norway

The current Comet Assay IV system works without any trouble…we are pleased with the system.

Scientist, Chemical Company, Japan

I don't have any questions about the software as it has worked quite well for far we are very happy with the software.

Research Scientist, University, Australia

I have been using the Comet Assay IV Software. It is great! During my software validation, I realized how much easier and precise it is compared to our old software. It makes analyzing the comet slides more enjoyable

Scientist, Medical Research Centre, USA

Excellent, perfectly straightforward.

Scientist, University, UK

We have just recently utilized the system and are extremely pleased.

Assistant Professor, University, USA

We have installed the software and are using it without any problem. It's really simple! My PhD students are very happy with it.

Professor, University, Spain

It's an amazing program! Really useful!

Research Scientist, University, Brazil

We're now using the software and it works great. It has cut our time for comets by at least half.

Post Doctoral Fellow, National Institute of Health, USA

Thanks for your attention and great service. I am so glad that we have Comet IV.

Study Director, Contract Research Organisation, USA

...have been using it now for a couple of weeks and it seems to be working great. Very user friendly - I love it as does my intern!

Staff Chemist, Marine Laboratory, USA

We are very satisfied with your software.

Scientist, Cancer Research Institute, Slovakia

All is going well and the more I use the software the more I love the way it's so simple and intuitive...the comet software is running well and in increasing use.

Scientist, University, UK

It is, indeed, much quicker than the old image analysis system that we had. We are very pleased with it.

Senior Researcher, Public Health Agency, Finland

Perfect and very clear installation and training.

Study Director, Contract Research Organisation, Europe

Everything has gone very well with the system and we are very pleased with it.

Scientist, University, UK

...Everything is fine with the Comet Assay IV system...and I'm pretty happy about this new software with just clicking on the cells instead of drawing a frame around them

Researcher, Cancer Research Organisation, UK

So far, I'm very happy with the system.

Research Lecturer, University, UK

I'm really a fan of your system :)

Genetic Toxicologist, Global Pharmaceutical Company

...and the system is running great.

Medical Researcher, Cancer Research Institute, USA

Excellent, the training was very helpful and specific to in-house technicalities.

Lecturer, University, UK

Perfect and very clear installation and training!

Researcher, Contract Testing Laboratory, Europe

We are using the Comet Assay IV a lot (and our use is increasing). It's working very well.

Professor, Children's Research Hospital, USA

The system is working great.

Senior Research Scientist, Cancer Research Institute, UK

I love the software. Keep up the good work over there.

Study Director, Contract Research Organisation, USA