Papers from 2013
Evidence for different mechanisms of action behind the mutagenic effects of 4-NOPD and OPD: the role of DNA damage, oxidative stress and an imbalanced …
R Åsgård, S Haghdoost, SO Golkar, B Hellman… - Mutagenesis, 2013
... Before the image analysis, the slides were stained with ethidium bromide (30 µl/slide; 200mg/ml) and analysed in a fluorescence microscope using the image analysis software Comet Assay IV (Perceptive Instruments, UK). ...
Antioxidant and genoprotective effects of spent coffee extracts in human cells
J Bravo, L Arbillaga, M Paz de Peña, C Cid - Food and Chemical Toxicology, 2013
... NIKON). A total of 100 comets on each slide were scored through a computer-assisted image analysis (Comet assay IV, Perceptive Instruments), by measuring the percentage of DNA in the tail. 2.7. Statistical analysis. Data are ...
Neuroprotective effect of creatine against propionic acid toxicity in neuroblastoma SH-SY5Y cells in culture
A El-Ansary, G Abu-Shmais, A Al-Dbass - African Journal of Biotechnology, 2013
... IV Ver. 4.3 (Perceptive Instruments Ltd., UK). animal models for some neurological diseases such as HD. Through mitochondrial ... et al. (2000). Comet assay IV Ver. 4.3 (Perceptive Instruments Ltd., UK) was used for calculations. plexity of ...
A beverage of Asiatic plantain extracts alleviated postprandial oxidative stress in overweight hyperlipidemic subjects challenged with a high-fat meal: a preliminary study
Y Lim, KW Lee, JY Kim, O Kwon - Nutrition Research, 2013
... The images of DNA migration from the nucleus were measured using Comet 4.2 image analysis software (Perceptive Instruments, Suffolk, UK). The amount of damaged DNA was expressed as the percentage of DNA in the tail (tail intensity), the tail length, and the tail moment. ...
UV-C Treatment using a Dean Vortex Technology – Impact on Apple Juice Enzymes and Toxicological Potential
A Müller, K Briviba, V Gräf, R Greiner, C Herrmann… - Innovative Food Science & …, 2013
... Then DNA was stained with ethidium bromide. The percentage of fluorescence in the tail (tail intensity, %) was quantified using the imaging software of Perceptive Instruments (Halstead, UK). Results are given as means SD. 2.10. Statistical analysis. ...
Tyrosyl-DNA phosphodiesterase 1 (TDP1) and Poly (ADP-Ribose) Polymerase-1 (PARP1) deficiency are cytotoxic to rhabdomyosarcoma cells
HK Fam, C Walton, SA Mitra, M Chowdhury, N Osborne… - Molecular Cancer Research, 2013
... Page 13. 13 imaging system. A total of 100 comets were scored for each sample using the Comet Assay IV software (Perceptive Instruments). TDP1 knockdown TDP1 was knocked down transiently by transfecting 8x104 cells with 100nM of ...
Activation of cellular signaling by 8-oxoguanine DNA glycosylase-1-initiated DNA base excision repair
P German, P Szaniszlo, G Hajas, Z Radak, A Bacsi… - DNA Repair, 2013
... After neutralization in 0.4 mol/L Tris–HCl (pH 7.5) for 5 min at room temperature, the DNA was stained with 1 ng per mL SYBER green. Comets were evaluated using the Comet Assay IV v4.2 system (Perceptive Instruments, Suffolk, UK). ...
Disinfection and toxicological assessments of pulsed UV and pulsed-plasma gas-discharge treated-water containing the waterborne protozoan enteroparasite …
J Hayes, D Kirf, M Garvey, N Rowan - Journal of Microbiological Methods, 2013
... Following fixation in methanol, DNA migration was measured via fluorescence microscopy coupled to image analysis software (Comet IV, Perceptive Instruments, UK) and expressed as percentage tail DNA (% tail DNA) and as tail moment. ...
Base excision repair capacity in chronic renal failure patients undergoing hemodialysis treatment
E Stoyanova, S Pastor, E Coll, A Azqueta, AR Collins… - Cell Biochemistry and …, 2013
... Slides were stained in Tris-EDTA buffer with an appropriate concentration of SybrGold. To score the slides, the Comet IV (Perceptive Instruments) programme was used, and 30 randomly selected cells were analysed per gel. ...
Increased DNA Damage in Progression Of COPD: A Response By Poly (ADP-Ribose) Polymerase-1
I Oit-Wiscombe, L Virag, U Soomets, A Altraja - PLOS ONE, 2013
... Images of 100 randomly selected cells (50 cells from each of two replicate slides) were taken with Zeiss Axiolab microscope (Carl Zeiss, Oberkochen, Germany) and analysed using Comet Assay IV software (Perceptive Instruments Ltd., Suffolk, UK). ...
Immunotoxicity and genotoxicity testing of PLGA-PEO nanoparticles in human blood cell model
J Tulinska, A Kazimirova, M Kuricova, M Barancokova… - Nanotoxicology, 2013
... C). The slides were washed at 4°C for 8 min with PBS followed by 8 min in water and dried at room temperature. A day before image analysis (Comet IV, Perceptive Instruments) the slides were stained with SYBRGold (10 000 ...
A comparative performance test of standard, medium-and high-throughput comet assays
A Azqueta, KB Gutzkow, CC Priestley, S Meier… - Toxicology in Vitro, 2013
... A semi-automated image analysis system (Comet Assay IV; Perceptive Instruments) was used to evaluate 50 nucleoids per gel (or, 30 per gel in the 96-minigel format, laboratory A). Percentage of DNA in tail was used to evaluate each comet. ...
Cyto-genotoxic and oxidative effects of a continuous UV-C treatment of liquid egg products
PM de Souza, K Briviba, A Müller, A Fernández… - Food Chemistry, 2013
... (2008). The percentage of fluorescence in the tail (tail intensity, %) was assessed fluorimetrically (DM 400 B, Leica Microsystems) and then quantified using the imaging software of Perceptive Instruments (Halstead, UK). Results are given as mean ± SD. 2.3. Statistical analysis. ...
Relationship between DNA damage in sperm after ex vivo exposure and abnormal embryo development in the progeny of the three-spined stickleback
R Santos, M Palos-Ladeiro, A Besnard, JM Porcher… - Reproductive Toxicology, 2013
... After staining with 0.02% ethidium bromide solution, DNA damage of 100 randomly selected cells per slide was analyzed using an Axioskop epifluorescence microscope (Zeiss, Germany) and an image analysis system (Comet IV software, Perceptive Instruments Ltd., UK). ...
ZRX1, the first EGFR inhibitor-capecitabine based combi-molecule, requires carboxylesterase-mediated hydrolysis for optimal activity
M Ait-Tihyaty, Z Rachid, AL Larroque-Lombard… - Investigational New Drugs, 2013
... DNA damage was quantified using Comet Assay IV software (Perceptive Instruments, UK) and the degree of DNA damage expressed as a tail moment parameter (ie, the distance between the barycenter of the head and the tail of the comet multiplied by the percentage of DNA ...
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro
MC Botelho, C Costa, S Silva, S Costa, A Dhawan… - Biomedicine & …, 2013
... Image capture and analysis were performed with Comet Assay IV software (Perceptive Instruments); percentage of tail DNA (%T) was the DNA damage parameter evaluated according to what has been recommended by Kumaravel et al. [13]. ... . ...
Iron oxide nanoparticle toxicity testing using high throughput analysis and high content imaging
G Harris, T Palosaari, Z Magdolenova, M Mennecozzi… - Nanotoxicology, 2013
... and covering with a cover slip. A Leica fluorescence microscope was used to evaluate the comets using a semi- automated image analysis system (Comet Assay IV, Perceptive Instruments). 'Lysis' slides give a measure of SBs ...
Damage response of XRCC1 at sites of DNA single strand breaks is regulated by phosphorylation and ubiquitination after degradation of poly (ADP) ribose
L Wei, S Nakajima, CL Hsieh, S Kanno, M Masutani… - Journal of Cell Science, 2013
... for the time indicated. Average tail moments from 100 cells per sample were obtained using Comet Assay IV software (Perceptive Instruments), and data are shown as mean values from three independent experiments. Purification of the BRCT II domain of XRCC1 ...
Comet Assay and Air-Liquid Interface exposure system: a new combination to evaluate genotoxic effects of cigarette whole smoke in human lung cell lines
S Weber, M Hebestreit, LL Conroy, G Rodrigo - Toxicology in Vitro, 2013
... Vision Technologies GmbH, Stadtroda, Germany). Images of stained slides were analyzed with the Comet Assay Version IV Image Analysis System (Perceptive Instruments, Steeple Bumpstead, UK). DNA migration values were ...
The relative roles of DNA damage induced by UVA irradiation in human cells
B Cortat, CCM Garcia, A Quinet, AP Schuch… - … & Photobiological Sciences, 2013
... The image analysis was performed using a Zeiss fluorescence microscope (Axiovert 200) with a 510–560 nm filter and a barrier of 590 nm. Fifty comets on each slide were scored according to their tail moments using Comet 4.0 software (Perceptive Instruments, Suffolk, UK). ...
Genotoxicity and induction of DNA damage responsive genes by food-borne heterocyclic aromatic amines in human hepatoma HepG2 cells
M Pezdirc, B Žegura, M Filipic - Food and Chemical Toxicology, 2013
... (1988) with minor modifications (Žegura and Filipic, 2004). The slides were stained with ethidium bromide (5 µg/ml) and analyzed using a fluorescence microscope (Nikon, Eclipse 800) with the image analysis software (Comet IV, Perceptive Instruments). ...
Subchronic Exposure to Ethyl tertiary Butyl Ether Resulting in Genetic Damage in Aldh2 Knockout Mice
Z Weng, M Suda, K Ohtani, N Mei, T Kawamoto… - Toxicology, 2013
... Each circle of the slides were then stained with 50 µl SYBR Green l (Trevigen) for 5 min. Slides were scored using a Comet IV capture system (Perceptive Instruments, Suffolk, England) and 100 cells were scored per sample. ...
Sulforaphane can protect lens cells against oxidative stress: implications for cataract prevention
H Liu, AJO Smith, MC Lott, Y Bao, RP Bowater… - … Ophthalmology & Visual …, 2013
... 100 comets were randomly analysed per sample (50 per gel), with images captured by fluorescence microscopy (Zeiss, cambridge, UK) and comets scored using Comet Assay IV Lite analysis software (Perceptive Instruments, Bury St Edmunds, UK). Microarray analysis ...
Preliminary evaluation of a model of stimulant use, oxidative damage and executive dysfunction
T Winhusen, J Walker, G Brigham, D Lewis, E Somoza… - The American Journal of …, 2013
... HCl) and stained with 40 µl/pad ethidium bromide (50 µg/ml). The tail length analysis was done on a fluorescent microscope (Nikon, Melville, NY) supported by the COMET ASSAY IV software (Perceptive Instruments, Haverhill, UK). ...
Interaction between DNA Polymerase ß and BRCA1
A Masaoka, NR Gassman, JK Horton, PS Kedar… - PLOS ONE, 2013
... All slides were coded prior to evaluation. After staining slides with SYBR TM Gold (Molecular Probes, Invitrogen), 100 cells were scored per slide using Comet Assay IV image analysis software Version 4.11 (Perceptive Instruments, Ltd., Suffolk, UK). ...
Effect of ß-carotene on catechol-induced genotoxicity in vitro: Evidence of both enhanced and reduced DNA damage
R Åsgård, B Hellman - Free Radical Research, 2013
... Before the image analysis the slides were stained with ethidium bromide (30 µl/slide; 200 mg/ml) and analyzed in a fluorescence microscope using the image analysis software Comet Assay IV (Perceptive Instruments, UK). Fifty comets per gel were captured for image analysis. ...
Mutagenicity and DNA damage of bisphenol A and its structural analogues in HepG2 cells.
A Fic, M Sollner Dolenc, M Filipic, L Peterlin Mašic - Arhiv za higijenu rada i …, 2013
... stained with EtBr (5 µg mL-1). Images of 50 randomly selected nuclei per experimental point were captured using a fluorescence microscope (Eclipse 800, Nikon, Tokyo, Japan) and analysed with image analysis software (Comet Assay IV, Perceptive Instruments, Suffolk, UK). ...
Mechanism study of goldenseal-associated DNA damage
S Chen, L Wan, L Couch, H Lin, Y Li, VN Dobrovolsky… - Toxicology Letters, 2013
... analysis. The slides were scored using an imaging analysis system consisting of a Nikon 501 fluorescent microscope (Melville, NY) and a Comet Assay IV digital analysis system (Perceptive Instruments Ltd., Edmunds, UK). ...
Parental exposure to methyl methane sulfonate of three-spined stickleback: contribution of DNA damage in male and female germ cells to further development …
R Santos, M Palos-Ladeiro, A Besnard, J Reggio… - Ecotoxicology
... After staining with 0.02 % ethidium bromide solution, DNA damage of 100 ran- domly selected cells per slide was analyzed using an Axio- skop epifluorescence microscope (Zeiss, Germany) and an image analysis system (Comet IV software, Perceptive Instruments Ltd., UK). ...
Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay
S Kolarevic, J Kneževic-Vukcevic, M Paunovic… - Chemosphere, 2013
... nm. Microscopic images of the comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). From each sample, 1–3 slides were prepared and the images of 50 cells from each slide were scored. Among ...
The Influence of Lentinan on the Capacity of Repair of DNA Damage and Apoptosis Induced by Paclitaxel in Mouse Bone Marrow Cells
SM Attia, GI Harisa, AR Abd-Allah, SF Ahmad… - Journal of Biochemical and …, 2013
... The slides were stained with ethidium bromide and studied by using a fluorescent microscope equipped with appropriate filter. Images from 100 cells were randomly selected for analysis using Comet Assay IV (Perceptive Instruments, Suffolk, UK) software. ...
Functional deletion of Txndc2 and Txndc3 increases the susceptibility of spermatozoa to age-related oxidative stress
TB Smith, MA Baker, HS Connaughton, U Habenicht… - Free Radical Biology and …, 2013
... At least 100 cells were analyzed per slide using Comet Assay IV Version 4.2 (Perceptive Instruments, Haverhill, Suffolk, UK), recording tail intensity and tail moment, but excluding cells that did not lyse completely. All samples were run in duplicate. CMA3. ...
Ionizing Radiation Promotes the Acquisition of a Senescence-Associated Secretory Phenotype and Impairs Angiogenic Capacity in Cerebromicrovascular Endothelial …
Z Ungvari, A Podlutsky, D Sosnowska, Z Tucsek, P Toth… - The Journals of Gerontology …, 2013
... 20 magnification. DNA damage was quantified by measuring the tail DNA content (as a percentage of total DNA) using the Comet Assay-IV software (Perceptive Instruments, Haverhill, Suffolk, United Kingdom). For assessment ...
Characterization of formaldehyde's genotoxic mode of action by gene expression analysis in TK6 cells
S Kuehner, K Holzmann, G Speit - Archives of Toxicology, 2013
... Images of 50 randomly selected cells were analyzed from one slide per culture. Measurements were made by image analysis (Comet Assay IV, Perceptive Instruments, Haverhill, UK), and DnA migration was determined by measuring the “tail intensity” (% tail DnA). ...
Hyperthermia–induced genotoxic effects in human A549 cells
G Speit, P Schütz - … Research/Fundamental and Molecular Mechanisms of …, 2013
... Measurements were made by image analysis (Comet Assay IV, Perceptive Instruments, Haverhill, UK) and DNA migration was determined by measuring the “tail intensity” (% tail DNA). 2.4. Fluorochrome-mediated viability test. ...
Potentiating toxicological interaction of single-walled carbon nanotubes with dissolved metals
M Al-Shaeri, D Ahmed, F Mc Cluskey, G Turner… - … Toxicology and Chemistry, 2013
... DNA damage was expressed as % tail DNA [33] and determined using the image analysis software package Comet Assay IV (Perceptive Instruments). Following arcsin transformation, the data were statistically analyzed as described above. ...
Protective effects of coffee against induction of DNA damage and pre-neoplastic foci by aflatoxin B1
F Ferk, WW Huber, B Grasl-Kraupp, K Speer… - Molecular Nutrition & Food …, 2013
... DNA migration (% DNA in tail) was determined with a computer-aided comet assay image analysis system (Comet Assay IV, Perceptive Instruments, UK). The experiments were carried out according to the international guidelines for SCGE experiments [23]. ...
Methionine concentration in the diet has a tissue-specific effect on chromosomal stability in female mice
AF Aissa, TDUH Gomes, MR Almeida, LC Hernandes… - Food and Chemical …, 2013
... A total of 100 nucleoids per animal were randomly examined at 400× magnification under the fluorescence microscope, equipped with 515–560-nm excitation filter and a 590-nm barrier filter. The software Comet Assay IV™ version 4.3 (Perceptive Instruments, Bury St. ...
Zinc ferrite nanoparticles activate IL-1b, NFKB1, CCL21 and NOS2 signaling to induce mitochondrial dependent intrinsic apoptotic …
Q Saquib, AA Al-Khedhairy, J Ahmad, MA Siddiqui… - Toxicology and Applied …, 2013
... CCD) camera. Images from 50 cells (25 from each replicate slide) were randomly selected and subjected to image analysis using software Comet Assay IV (Perceptive Instruments, Suffolk, UK). Western blotting. WISH cells treated ...
A method for the assessment of DNA damage in individual, one day old, zebrafish embryo (Danio rerio), without prior cell isolation
T Eleršek, J Plazar, M Filipic - Toxicology in Vitro, 2013
... The slides were neutralized with 0.4 M Tris buffer (pH 7.5), stained with ethidium bromide (5 mgL -1 ) and analyzed using a fluorescence microscope (Nikon, Eclipse 800). The% tail DNA was measured with image analysis software (Comet IV, Perceptive Instruments). ...
Intracellular deoxyribonucleotide pool imbalance and DNA damage in cells treated with hydroxyurea, an inhibitor of ribonucleotide reductase
SO Golkar, S Czene, A Gokarakonda, S Haghdoost - Mutagenesis, 2013
... After staining with propidium iodide (15 µg/ml in distilled water), DNA migration was measured using an interactive live video-based system for scoring DNA migration parameters at single cell level (Comet Assay IV; Perceptive Instruments Ltd). ...
Antigenotoxic Effect of Tartary (Fagopyrum tataricum) and Common (Fagopyrum esculentum) Buckwheat Flour
M Vogrincic, I Kreft, M Filipic, B Žegura - Journal of Medicinal Food, 2013
... Images of 50 randomly selected nuclei per slide were analyzed with image-analysis software Comet Assay IV (Perceptive Instruments, Bury St Edmunds, United Kingdom). The percentage of tail DNA was used as the measure of DNA damage. ...
Tissue differences in BER-related incision activity and non-specific nuclease activity as measured by the comet assay
JP Gorniak, KM Cameron, KJ Waldron, T von Zglinicki… - Mutagenesis, 2013
... Slides were left to dry overnight and were scored the following day. Comets were visualised using an Olympus BX51 fluorescence microscope and 50 comets/gel were analysed using the Comet Assay IV software (Perceptive Instruments). ...
Overtraining is associated with DNA damage in blood and skeletal muscle cells of Swiss mice
JBC Pereira, JR Pauli, LM Antunes, EC de Freitas… - BMC physiology, 2013
... One hundred nucleoids per animal (50 nucleoids per slide) were analyzed using Comet Assay IV software (Perceptive Instruments, Haverhill, UK). While undamaged cells have an intact nucleus without a tail, damaged cells have the appearance of a comet. ...
In vivo comet assay of a novel galacto-oligosaccharide in rats
K Narumi, H Takasawa, W Ohyama, K Kaneko - Human & Experimental Toxicology, 2013
... Images of cell nuclei were captured with a fluorescent microscope (at 200 × magnification with B excitation) and subsequently analyzed using a comet image analyzer (comet assay IV, Perceptive Instruments Ltd, Suffolk, UK). ...
Comet assay in reconstructed 3D human epidermal skin models—investigation of intra-and inter-laboratory reproducibility with coded chemicals
AA Reus, K Reisinger, TR Downs, GJ Carr, A Zeller… - Mutagenesis, 2013
... EDTA in distilled water, pH 7.5)]. At least two slides per skin model were analysed. Fifty nuclei per slide were randomly measured with Comet Assay IV software (Perceptive Instruments, Suffolk, UK). Even if a slide showed a ...
Atmospheric pressure plasma jet treatment evokes transient oxidative stress in HaCaT keratinocytes and influences cell physiology
K Wende, S Straßenburg, B Haertel, M Harms, S Holtz… - Cell Biology International, 2013
... After drying, DNA was stained with ethidium bromide (20 µg/mL) and analyzed by fluorescence microscopy (Olympus CK 40, green filter cube), using software Comet Assay IV (Perceptive Instruments, Suffolk, United Kingdom). Fifty randomly selected cells of each ...
Analysis of DNA Damage and Repair by Comet Fluorescence In Situ Hybridization (Comet-FISH)
M Glei, W Schlörmann - Functional Analysis of DNA and Chromatin, 2014
... 6. Digital CCD camera and imaging software (eg, MicroMAX, BFI OPTILAS GmbH; Visitron Systems GmbH, Puchheim, Germany). 7. Image analysis system (eg, Kinetic Imaging, Liverpool, UK or Perceptive Instruments Suffolk, UK). 3 Method. 3.1 Pre-coating of Slides. ...
Mutant frequency in comparison to oxidative DNA damage induced by ochratoxin A in L5178Y tk+/-(3.7. 2C) mouse lymphoma cells
R Ali, X Guo, H Lin, QM Khan, M Ismail, U Waheed… - Drug and Chemical …, 2013
... and scored for each dose-analysis-method combination, and at least 100 cells were selected randomly from each slide and scored using a system consisting of a Nikon 501 fluorescent microscope and Comet IV digital imaging software (Perceptive Instruments, Wiltshire, UK). ...
Base excision DNA repair defect in thioredoxin-1 (Trx1)-deficient cells
HL Kim, P Koedrith, SM Lee, YJ Kim, YR Seo - Mutation Research/Fundamental and …, 2013
... For quantitative scoring, 100 cells per slide were visualized under a fluorescent microscope (Nikon, Japan) with 200× magnification and scored using Comet IV software (Perceptive Instruments, UK). 2.9. Quantitative real-time RT-PCR. ...
Evaluation of the genotoxicity of clomiphene citrate
S Yilmaz, F Ünal, E Yilmaz, D Yüzbasioglu, SE Ilhan - Mutation Research/Genetic …, 2013
... The tail length and tail intensity (%) of one hundred comets on each slide were determined (totally 400 comets per concentration, duplicate slides per treatment) with a specialized image-analysis system (“Comet Assay IV”, Perceptive Instruments Ltd., UK). 2.5. ...
Genotoxicity assessment of some cosmetic and food additives
A Di Sotto, F Maffei, P Hrelia, S Di Giacomo, E Pagano… - Regulatory Toxicology and …, 2013
... After neutralisation in Tris–HCl (0.4 M, pH 7.5), the gels were stained with ethidium bromide (20 µg/ml). Images were analysed using a Leica microscope equipped with Comet Assay TM software (Perceptive Instruments, UK). 2.7. Statistical analysis. ...
In vivo and in vitro genotoxicity assessment of 2-methylisoborneol, causal agent of earthy–musty taste and odor in water
L Burgos, M Lehmann, HHR de Andrade… - … and Environmental Safety, 2013
... The stained nucleoids were immediately evaluated at 400× magnification under an Olympus (BX41) fluorescence microscope (excitation filter, 515–560 nm; barrier filter, 590 nm), equipped with an image analysis system (Comet Assay IV, Perceptive Instruments, UK). ...
Coating-dependent induction of cytotoxicity and genotoxicity of iron oxide nanoparticles
Z Magdolenova, M Drlickova, K Henjum… - Nanotoxicology, 2013
... Quantification was done using image analysis system Comet assay IV (Perceptive Instruments Ltd.), scoring 100 comets per sample. As positive controls, cells were treated with 50 µM H 2 O 2 in PBS (4 °C) for 5 min on ice. Statistical analysis ...
Protective action of n-3 fatty acids on benzo [a] pyrene-induced apoptosis through the plasma membrane remodeling-dependent NHE1 pathway
B Dendelé, X Tekpli, K Hardonniere, JA Holme… - Chemico-Biological …, 2013
... as described previously [30]. Nuclei were stained with ethidium bromide and DNA damage scored (50 nuclei per sample) using the Comet IV capture system (version 4.11; Perceptive Instruments, UK). The tail intensity (% tail ...
Comet assay reveals no genotoxicity risk of cationic solid lipid nanoparticles
S Doktorovova, AM Silva, I Gaivão, EB Souto… - Journal of Applied …, 2013
... described above. Comet Assay Evaluation. Comets were observed using a Nikon Eclipse E400 microscope and evaluated using Comet Assay IV software (Perceptive Instruments, UK), after ethidium bromide staining. The tail ...
The Extreme Variety of Genotoxic Response to Benzo[a]pyrene in Three Different Human Cell Lines from Three Different Organs.
Genies C, Maître A, Lefèbvre E, Jullien A, Chopard-Lallier M, Douki T. PLoS One. 2013
...assay, slides were analyzed with a fluorescence microscope coupled with a computer system using Comet Assay IV software. We randomly picked 50 comets on each slide and the percentage of tail intensity (% tail ...
Ochratoxin A induces DNA double-strand breaks and large deletion mutations in the carcinogenic target site of gpt delta rats.
Kuroda K, Hibi D, Ishii Y, Takasu S,...Mutagenesis. 2013
...subjected to image analysis using Comet Assay IV software (Perceptive Instruments Ltd, Bury St Edmunds, UK). ...
Parental exposure to methyl methane sulfonate of three-spined stickleback: contribution of DNA damage in male and female germ cells to further development impairment in progeny
R. Santos, M. Palos-Ladeiro, A. Besnard, J. Reggio, E. Vulliet, J. M. Porcher, S. Bony, W. Sanchez, A. Devaux
... After staining with 0.02 % ethidium bromide solution, DNA damage of 100 randomly selected cells per slide was analyzed using an Axioskop epifluorescence microscope (Zeiss, Germany) and an image analysis system (Comet IV software, Perceptive Instruments Ltd., UK). ...
Leucocytes isolated from simply frozen whole blood can be used in human biomonitoring for DNA damage measurement with the comet assay
MB Akor-Dewu, N El Yamani, O Bilyk, L Holtung… - Cell Biochemistry and …, 2013
.... with agitation, for 30 min at 4°C, in the dark. They were then washed twice with water and left to dry. Comets were scored with Comet Assay IV software (Perceptive Instruments) and results expressed as % tail DNA.
Lobarstin Enhances Chemosensitivity in Human Glioblastoma T98G Cells
S KIM, S JO, H LEE, TAEUE KIM, ILC KIM, JHAN YIM… - Anticancer Research, 2013
... Images obtained by fluorescence microscopy (Olympus IX71 from Olympus; Tokyo, Japan) were subjected to analysis with Comet Assay IV v4.3 (Perceptive Instruments; Suffolk, United Kingdom). Statistical analysis of the results was performed as recommended by Bright et al. ...
TDP1 deficiency sensitizes human cells to base damage via distinct topoisomerase I and PARP mechanisms with potential applications for cancer therapy
M Alagoz, OS Wells, SF El-Khamisy - Nucleic Acids Research, 2013
... Average tail moments from 50 cells/sample were measured using Comet Assay IV software (Perceptive Instruments, UK). Data are the average ± sem from three independent experiments. Statistical analyses were conducted using student t-test. ...
Genotoxic evaluation of chloroacetonitrile in murine marrow cells and effects on DNA damage repair gene expressions
SM Attia, SF Ahmad, KM Zoheir, SA Bakheet, GK Helal… - Mutagenesis, 2013
... Images from 100 cells (50 from each replicate slide) were randomly selected and subjected to image analysis using software Comet Assay IV (Perceptive Instruments, Suffolk, UK). The extent of primary DNA strand breaking can be quantified by the use of tail intensity (%). ...
Daily Nutritional Dose Supplementation with Antioxidant Nutrients and Phytochemicals Improves DNA and LDL Stability: A Double-Blind, Randomized, and Placebo-Controlled Trial
YJ Kim, YH Ahn, Y Lim, JY Kim, J Kim, O Kwon - Nutrients, 2013
... The slides were evaluated under a fluorescence microscope (Nikon, Tokyo, Japan). Images from at least 50 comets on each slide were analyzed with COMET 4.2 image analysis software (Perceptive Instruments, Suffolk, UK). The ...
Investigations on potential co-mutagenic effects of formaldehyde
G Speit, R Linsenmeyer, G Duong, J Bausinger - Mutation Research/Fundamental …, 2013
... Measurements were made by image analysis (Comet Assay IV, Perceptive Instruments, Haverhill, UK) and DNA migration was determined by measuring the tail moment to enable a direct comparison with previously published results [13] and [14]. ...
Topoisomerase-II-targeting properties of a grapevine-shoot extract and respective resveratrol oligomers
SA Bächler, A Schroeter, M Dicker, P Steinberg… - Journal of Agricultural and … 2013
... the comet tail calculated as percentage of overall DNA intensity) was analyzed scoring 50 images per slide with the CometIV software (Perceptive Instruments, Suffolk, Great Britain). Statistical analysis 163 Data were expressed as mean ± SD. ...
Senescence of Primary Amniotic Cells via Oxidative DNA Damage
R Menon, I Boldogh, R Urrabaz-Garza, J Polettini… - PLOS ONE, 2013
... at 37°C. Electrophoresis was conducted for 30 min at 21 V. Fifty cells per culture were counted under an Olympus microscope (40× objectives) and scoring of the comet tail DNA content was performed using the Comet Assay IV v4.2 system (Perceptive Instruments, Suffolk, UK). ...
Gap-filling and bypass at the replication fork are both active mechanisms for tolerance of low-dose ultraviolet-induced DNA damage in the human genome
A Quinet, AT Vessoni, CRR Rocha, V Gottifredi, D Biard… - DNA Repair, 2013
... Finally, the slides were stained with ethidium bromide and imaged with a fluorescence microscope (Axiovert 200, Zeiss) at a magnification of 400×. At least 100 comets per slide were scored with the Comet Assay IV software (Perceptive Instruments). 2.9. Statistical analysis. ...
Pyruvate remediation of cell stress and genotoxicity induced by haloacetic acid drinking water disinfection by-products
A Dad, CH Jeong, JA Pals, ED Wagner… - Environmental and …, 2013
... 25 randomly selected nuclei per microgel were analyzed under a Zeiss fluorescence microscope with the Comet IV imaging system (Perspective Instruments, Suffolk ... image ... GAPDH inhibition kinetics were highly correlated with a host of toxicity metrics (Table 4) [Pals et al., 2011]. ...
Cell cycle alterations induced by urban PM2.5 in bronchial epithelial cells: characterization of the process and possible mechanisms involved.
Longhin E, Holme JA, Gutzkow KB, Arlt VM, Kucab JE, Camatini M, Gualtieri M. Part Fibre Toxicol. 2013
Rehydrated samples were stained with SybrGold (0.08 µl/ml in TE-buffer pH 7.4, 20 min) and scored with Perceptives Comet IV software. The level of DNA damage was expressed as tail intensity, i.e. percent fluorescence in the comet tail, relative to the comet total fluorescence.
Intracellular deoxyribonucleotide pool imbalance and DNA damage in cells treated with hydroxyurea, an inhibitor of ribonucleotide reductase
AOsterman Golkar S1, Czene S, Gokarakonda A, Haghdoost S. Mutagenesis. 2013
migration parameters at single cell level (Comet Assay IV; Perceptive Instruments Ltd). The observations of DNA migration were made at 25× magnification using a fluorescence microscope equipped with a 515–560-nm excitation filter. Fifty cells per comet sample were randomly